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Isolation of Bacillus sp. Producing Poly-$\gamma$-glutamic Acid with High Efficiency and Its Characterization  

You Kyung-Ok (Department of Life Science and Biotechnology, Dong-Eui University)
Oh You-Na (Department of Life Science and Biotechnology, Dong-Eui University)
Kim Byung-Woo (Department of Life Science and Biotechnology, Dong-Eui University)
Nam Soo-Wan (Department of Biotechnology and Bioengineering, Dong-Eui University)
Jeon Sung-Jong (Department of Biotechnology and Bioengineering, Dong-Eui University)
Kim Dong-Eun (Department of Biotechnology and Bioengineering, Dong-Eui University)
Kim Young-Man (Department of Food and Nutrition/Oriental Biotech. Co., Dong-Eui University)
Kwon Hyun-Ju (Department of Life Science and Biotechnology, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.3, 2005 , pp. 200-206 More about this Journal
Abstract
A bacterium with high productivity of poly-$\gamma$-glutamic acid (PGA) was isolated from the traditional Korean seasoning, ChungKookJang. The 16s ribosomal RNA sequence of isolated strain showed 97.6, 98.9 and $90.3{\%}$ of similarity to those of Bacillus sp. WL-3, Bacillus subtilis; ENV1 and B amy-loliquefaciens (T), respectively. Accordingly, this bacterium was identified as a Bacillus sp. However, some biochemical characteristics of this strain were different from those of B. subtilis: D-xylose fermentation and glycogen utility were negative. Maximum production of PGA was achieved when it was grown aerobically in a culture medium containing glutamic acid ($3{\%}$) and fructose ($4{\%}$) as carbon sources. The volumetric yield of PGA reached up to 27 g/l in the optimum culture medium. These results suggest that the present strain can be applicable for industrial purposes such as a prototype strain for food or cosmetics industry.
Keywords
Bacillus sp.; fructose; 16s rRNA; ChungKookJang;
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