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Detection of cry-type Genes of Bacillus thuringiensis Isolates from Korea  

Park Sooil (Department of Biological Sciences, Konkuk University)
Lee Kwang Yong (Department of Biological Sciences, Konkuk University)
Kang Eun Young (Department of Biological Sciences, Konkuk University)
Kim Eui Na (Department of Biological Sciences, Konkuk University)
Kwon Hyuk Han (Department of Biological Sciences, Konkuk University)
Ahn Seong Kyu (Department of Biological Sciences, Konkuk University)
Lee Hyung Hoan (Department of Biological Sciences, Konkuk University)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.2, 2005 , pp. 154-158 More about this Journal
Abstract
Twenty-three Bacillus thuringiensis strains isolated from Korea were screened to detect the cry-type genes using PCR with 21 specific oligonucleotide primers. Eight strains contained distinct multiple crystal genes; cry1Aa2, cry1Ab1, cry1Ac1 and cry2Aa1. These results indicate that the strains coincided with the B. thuringiensis subsp. kurstaki strain. The other 15 strains were not recognised to the 21 specific primers.
Keywords
Bacillus thuringiensis; crystal; crystal gene; PCR;
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