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Synergistic Antimicrobial Effect of Patrinia scabiosaefolia and Forsythiae fructus Extracts on Food-borne Pathogens  

Bae Ji-Hyun (Department of Food Science and Nutrition, Keimyung University)
Son Kug-Hee (Department of Food Science and Nutrition, Keimyung University)
Lee Eun-Joo (Department of Food Science and Nutrition, Keimyung University)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.2, 2005 , pp. 130-135 More about this Journal
Abstract
To investigate the antimicrobial effect of the Patrinia scabiosaefolia extracts against food-borne pathogens, we extracted the P. scabiosaefolia with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, ethyl acetate, and methanol, respectively. The antimicrobial activity of the P. scabiosaefolia extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of P. scabiosaefolia showed the highest antimicrobial activity against Escherichia coli and Shigella sonnei. Synergistic effect in inhibition was observed when P. scabiosaefolia extract was mixed Forsythiae fructus extract as compared to each extracts alone. Finally, the growth inhibition curves were determined by using ethyl acetate extracts of P. scabiosaefolia against Staphylococcus epidermidis and Shigella sonnei. The ethyl acetate extract of P. scabiosaefolia had strong antimicrobial activity against S. sonnei at the concentration of 4,000 ppm. At this concentration, the growth of S. Sonnei was retarded more than 72 hours and up to 48 hours for S. epidermidis. These results suggest that the ethyl acetate extracts of P. scabiosaefolia can be used for the efficient material against the growth of S. epidermidis and S. sonnei.
Keywords
Patrinia scabiosaefolia; antimicrobial activity; food-borne pathogens;
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