Browse > Article

Isolation and Culture Conditions of a Pseudomonas Strain Capable of Removing $NH_4^+\;and\; NO_3^-$ Simultaneously in Anaerobic Conditions  

Kim Young-Ju (Department of Civil and Environmental Engineering, University of Ulsan)
Song Young Chae (Division of Civil and Environmental System Engineering, Korea Maritime University)
Kim Jong Oh (Division of Construction Engineering Kyeongsang Nation University)
Park Hung-Suck (Department of Civil and Environmental Engineering, University of Ulsan)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.1, 2005 , pp. 65-69 More about this Journal
Abstract
A bacterial strain AE-1-3, isolated from soil and wastewater identified as Pseudomonas strain, removed $NH_4^+\;and\; NO_3^-$ simultaneously in anaerobic cultivation in a medium containing $0.1\%\;NH_4NO_3\;and\;3.0\%$ glucose. The strain removed $NH_4^+\;,\;NO_3^-\;and\;NO_2^-$ completely in 15 days of anaerobic cultivation. Though N03- removed completely, $33\%\;of\;NH_4^+$ remained in 15 day of incubation in $1\%$ glucose and $0.1\%\;NH_4NO_3$ medium. The bacterium could remove $0.1\%\;NH_4NO_3$ completely in a short time by addition of $Cu^{2+},\;Zn^{2+},\;Sn^{2+}\;in\;0.5\%$ glucose medium. By chaning the metal concentration, $0.3\%\;NH_4NO_3$ could be removed completely.
Keywords
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Jetten, M. S. M., M. Sttous, K. T. van de Pas-schoonen, J. Schalk, U.G J. M. van Dongen, M. M. C. van Loosdrecht, and J. G Kuenen. 1999. The anaerobic oxidation of ammonium. FEMS. Microbiol. Rev. 22: 421-437   DOI   ScienceOn
2 Kim, Y-J., M. Yosizawa, S. Takenaka, S. Murakami, and K. Aoki. 2002. Ammonia assimilation in Klebsiella pneumoniae F-5-2 that can utilize ammonium and nitrate ions simultaneously: Purification and characterization of glutamate dehydrogenase and glutamine systhetase. J. Biosei. Bioeng, 193: 584-588
3 Kizai, H. M. 1997. Bukino tiltuso to ryoujou no ntaisya. Tokyokagakutouinn, pp. 258-260. In Saiboukinou totaisya Map, Japan
4 Kim, Y-J. 2002. Metabolism of ammonium and nitrate ions by Klebsiella pneumoniae F-5-2 under anaerobic conditions. Kobe University, 43-52
5 Kim, Y-J., M. Yosizawa, S. Takenaka, S. Murakami, and K. Aoki. 2002. Isolation and cultural conditions of a Klebsiella pneumoniae strain that can utilize ammonium and nitrate ions simultaneously under controlled iron and molybdate ions. Biosci. Biotechnol. Biochem. 66: 996-1001
6 Maartje, D., T. Vasiliky, GInes, L. Rosa, V. Carlos, and V. Javier. 2001. Effect of heavy metals on nitrate assimilation in the eukaryotic microalga Chlamydomonas reinhardtii. Plant Physiol. Biochem. 39: 443-448   DOI   ScienceOn
7 Strous, M., J. A Fuerst, F. H. M K. Ramer, S. logemann, G Muyzer, van de Pas-Schoonen, K. T., Kuenen, J. G, and M. S. M. Jetten, 1999. Missing lithotroph identified as new planctomycete. Nature 400: 446-449   DOI   ScienceOn
8 Koenig, A. and L. H. Liu. 2001. Kinetic model of autotrophic denitrification in sulphur packed-bed reactors. Wat. Res. 35: 1969-1978   DOI   ScienceOn
9 Ingo, S., S. Olav, M. Schmid, E. Bock, J. Fuerst, J. G Kuenen, M. S. M. Jetten, and M. Strous. 2003. New concepts of microbial treatment processes for the nitrogen removal in wastewater. FEMS Microbiol. Rev. 27: 481-492   DOI   ScienceOn
10 Anzai, Y, H. Kim, J. Y Park, H. Wakabayashi, and H. Oyaizu. 2000. Phylogenetic affiliation of the Pseudomonas based on 16S rRNA sequence. Int. J. Syst. Evol. Microbiol. 50: 1563-1589   DOI   PUBMED   ScienceOn
11 van Dongen, L.G J. M., M. S. M. Jetten, and M.C.M. van Loosdrecht. 2001. The combined Sharon/Anamox process. Found. Appl. Wat. Res. 23-29
12 APHA, 1998. Standard Methods for the examination of water and wastewater 20th ed., New York
13 Francisco, J. G, D. A. De la Rosa, and J. Gomez. 2001. nitrogen removal from wastewater at low C/N ratios with ammonium and acetate as electron donors. Biores. Technol. 79: 165-170   DOI   ScienceOn