Browse > Article

Optimization of Extraction Conditions of Antioxidants from Sporophyll of Undaria pinnatifida by Response Surface Methodology  

Yu, Mi-Yeong (Division of Food science & Biotechnolology, Pukyong National University)
Kim, Gyeong-Hwan (Division of Food science & Biotechnolology, Pukyong National University)
Lee, Jae-U (Department of Food Preparation, Changwon College)
Kim, Sang-Gwon (Chung-Ho Sea Food Co., Ltd)
Yang, Ji-Yeong (Division of Food science & Biotechnolology, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.32, no.4, 2004 , pp. 317-321 More about this Journal
Abstract
We selected a solvent for producing antioxidants from sporophyll of Undaria pinnatOptimum extraction condition for antioxidant ability(Y) of sporophyll of U. pinnatifida was investigated using response surface methodology(RSM). A compound central design was used with variables ($X_1$)temperature($^{\circ}C$) (3.4; 20; 60; 100; 116.6); ($X_2$) pH(1.8; 3; 6; 9; 10.2) and ($X_3$) treatment time(min) (1.7; 10; 30; 50; 58.3). Antioxidant activities were increased with decreasing pH and increasing temperature. The optimal extraction conditions for antioxidant ability(Y) of sporophyll of U. pinnatifida were found to be temperature $51.55^{\circ}C$, pH 4.2 and treatment time 28.2 min. Among the variables tested, pH showed greater significant facin extraction of antioxidants from sporophyll of U. pinnatifida.
Keywords
Sporophyll; Undaria pinnatifida; antioxidant activity; response surface methodology;
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Hong, J. S., Y. J. Kwon, Y. H. Kim, M. K. Kim, I. W. Park, and K. H. Kang. 1991. Fatty acid composition of miyeok (Undaria pannatifida) and pare(Enteromorpha compressa). J. Kor. Soc. Food Nutr. 20: 376-380
2 Koo, J. G. 2001. Biosorption of lead and cadmium by fucoidan from Undaria pannatifida. J. Kor. Fish. Soc. 34: 521-525
3 Lim S. N., P. C. K. Cheung, V. E. C. Ooi, and P. O. Ang. 2002. Evaluation of antioxidant of extract from a brown seaweed, Sargassum siliauastrum. J. Agric. Food Chem. 50: 3862-3866
4 Sopheak, S. and L. Betty. 2002. Free radical scavenging activity of caffeic acid amide and ester analogues: structureactivity relationship. J. Agric. Food Chem. 50: 468-472
5 Nam, S. H., S. M.. Chang, and M. Y. Kang. 2003. Variated difference in antioxidative activity of ethanol extracts from colored rice bran. J. Kor. Soc. Chem. Biotechnol. 46: 10-22
6 Koo, J. G., Y. S. Choi, and J. K. Kwak. 2001. Blood-anticoagulant activity of fucoidan from sporophylls of Undaria pinnatifida. J. Kor. Fish. Soc. 34: 515-520
7 Smith, K. L. and J. L. Harwood. 1984. Lipid and lipid metabolism in the brown alga. Phytochemistry 23: 2469
8 Zamora, R., M. Alaiz, and F. J. Hidalgo. 2001. Influence of cultivar and fruit ripening on olive (Olea europaea) fruit protein content, composition, and antioxidant activity. J. Agric. Food Chem. 49: 4267-4270
9 Etsuo, N., S. Hiroyuki, and M. Makato. 1994. Antioxidants : free-radicals and biological defense, pp. 6-8, Japan Scientific Societies Press, Tokyo, Japan
10 Han, J., S. Kang, R. Choue, H. Kim, K. Leem, S. Chung, C. Kim, and J. Chung. 2002. Free radical scavenging effect of Diospyros kaki, Laminaria japonica and Undaria pinnatifida. Fitoterapia 73: 710-712
11 Kwon, E. J., Y. C. Kim, M. S. Kwon, C. S. Kim, W. W. Kang, J. B. Lee, and S. K. Chung. 2001. Antioxidative activity of solvent fraction and isolation of antioxidative compounds from chestnut husk. J. Kor. Soc. Food Sci. Nutr. 30: 726-731
12 Box, G. E. P. and J. S. Hounter. 1957. Multifactor experimental design for exploring response surface. Ann. Math. Stat. 28: 195-242
13 Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 26: 1199-1200
14 Ooishi, K. 1993. Science of Seaweed. pp. 61-62. Chochang Press, Tokyo, Japan
15 Osborn-Barnes H. T. and C. C. Akoh. 2003. Effects of $\alpha$- tocopherol, $\beta$-carotene, and isoflavones on lipid oxidation of strictured lipid-based emulsions. J. Agric. Food Chem. 51: 6856-6860
16 Cochran, W. G. and G. M. Cox. 1957. Experimental design, pp. 335-375. John Wiley & Sons Inc., New York, U.S.A