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Isolation and Characteristics of Polyhydroxyalkanoates Producing Pseudomonas sp. MBEL21  

최종일 (한국과학기술원 생명화학공학과, 생물공정연구센터 및 대사공학 국가지정연구실)
이승환 (한국과학기술원 생명화학공학과, 생물공정연구센터 및 대사공학 국가지정연구)
이상엽 (한국과학기술원 생명화학공학과, 생물공정연구센터 및 대사공학 국가지정연구실, 한국과학기술원 바이오시스템학과)
Publication Information
Microbiology and Biotechnology Letters / v.32, no.2, 2004 , pp. 123-127 More about this Journal
Abstract
Pseudomonas sp. MBEL21 was newly isolated from soil samples and found to accumulate medium-chain-length Polyhydroxyalkanoates(MCL-PHAs) using oleic acid as a sole carbon source. Among the various nutrient limiting conditions examined, including nitrogen, sulfur and phosphorus, only phosphorus limitation supported the accumulation of MCL-PHAs up to 15 wt％ of dry cell weight in flask cultures. MCL-PHAs produced by Pseudomonas sp. MBEL21 was mainly composed of 3-hydroxy-5-cis-tetradecenoate. Fed-batch culture of Pseudomonas sp. MBEL21 by novel feeding strategies based on cell growth charcteristics was carried out under phosphorus limitation using oleic acid as a sole carbon source. The final cell concentration and PHA content of 82 g/L and 28 wt％, respectively, were obtained. Furthermore, PHA consisted of MCL-hydroxyalkanoates and 3-hydroxybutyrate could be produced using olive oil as a sole carbon source.
Keywords
Pseudomonas sp. MBEL21; PHA; Oleic acid; olive oil;
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