Browse > Article

Purification and Characterization of Phospholipase D from Actionmycetes KF923  

곽보연 (한국식품개발연구원 식품기능연구본부)
윤석후 (한국식품개발연구원 식품기능연구본부)
김창진 (한국생명공학연구원)
손동화 (한국식품개발연구원 식품기능연구본부)
Publication Information
Microbiology and Biotechnology Letters / v.32, no.1, 2004 , pp. 78-83 More about this Journal
Abstract
In order to screen microorganisms producing phopholipase D (PLD) had high transphosphatidylation activity, about 1,000 Actinomycetes strains were isolated from the 63 soil samples, collected over 6 local area in Korea. When the hydrolytic activity in the supernatant was determined, 131 strains produced PLD more than 0.3U/$m\ell$. Among 131 culture broths tested, 23 ones had transphosphatidylation activity higher than 20% and finally one strain (Actinomycetes KF923), which had highest hydrolytic and transphophadylation activity, was selected. Actinomycetes KF923 showed the highest hydrolytic activity (13U/$m\ell$) and phosphatidylation activity (95%) after 48 h fermentation using the P medium (yeast extract 1%, peptone 1%, glucose 1.5%, glycerol 1%, $CaCO_3$ 0.4%, pH 7.2). PLD was purified from the culture broth of Actinomycetes KF923 and the specific activity of purified PLD was 567U/mg. The molecular weight of PLD was about 55kD and the optimum pH and temperature were pH 6.0 and $60^{\circ}C$, respectively. The stability of PLD toward pH and temperature were high around pH 8.0 and below $40^{\circ}C$ Special metal ions were not necessary to the PLD activity.
Keywords
Purification; characteristics; phospholipase D; Actinomycetes;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Phospholipase D Production using immobilized cells of Streptoverticillium cinnamoneum /
[ Fukuda,H.;Y.Turugida;T.Nakjima;E.Nomura;A.Kondo ] / Bitechnol. Lett.   ScienceOn
2 Purification and applications of a phospholipase D from a new strain of Streptomyces /
[ Carrea,G.;P.D'Arrigo;F.Secundo;S.Servi ] / Biotechnol. Lett.   ScienceOn
3 Reactions catalysed by lipid metabolizing enzymes /
[ Yamane,T. ] / Yukagaku
4 Cleavage of structural proteins during the assembly of head of bacteriophage T4 /
[ Laemmli,U.K. ] / Nature   ScienceOn
5 New material of oil. /
[ Anonym ] / Food Processing (Jpn)
6 Genomic structure, cloning and expression of two phospholipase D isoenzymes from white cabbage /
[ Schaffner I.;K.P.Rucknageil;J.Mansfeld;R.UlbrichHofmann ] / Eur. J. Lipid Sci. Technol.   ScienceOn
7 Continuous production of phospholipase D by Streptomyces lydicus D-121 immobilized with cross-linked chitosan beads /
[ Shinonaga.M.;Y.Kawamura;K.Shimbo;T.Yamane ] / J. Ferm. Bioeng.   ScienceOn
8 Enzymatic modification of soybean lecithin /
[ Aoi,N. ] / Bio Industry(Jpn)
9 Modification and application of lecithin by transfer enzyme /
[ Kudo,S.;A.Kuroda ] / Bio Industry(Jpn)
10 Purification and characterization of phospholipase D (PLD) from rice (Oryza sativa L.) and cloning of cDNA for PLD from rice and maize (Zea Mays L.) /
[ Ueki,J.;S.Morioka;T.Komari;T.Kumashiro ] / Plant Cell Physiol.
11 Screening of phospholipae D producing Actinomycetes /
[ Shon,D.H.;J.Y.Shim;S.H.Yoon ] / Kor. J. Appl. Microbiol. Biotechnol.
12 Transphosphatidalation by immobilized phospholipase D in Aqueous media /
[ Dittrich,N.;R.Ulbrich-Hofmann ] / biotechnol. Appl. Biochem   ScienceOn
13 Purification and properties of phospholipase D from Steptomyces antibioticus /
[ Shimbo,K.;Y.Iwasaki;T.Yamane;K.Ina ] / Biosci. Biotech. Biochem.
14 Preparative transformation of natural phospolipids catalyzed by phospholipase D from Streptomyces /
[ D'Arrigo,D.;L.de Ferra;V.Piergianni;A.Selva;S.Servi;A.Strini ] / J. Chem. Perkin Trans I
15 Nucleosides and nucleotides. 150. Enzymic synthesis of 5'=phosphatidyl derivatives of 1-(2-C-cyano-2-deoxy-beta-D-arabino-pentofuranosyl cytosine (CNDAC) and their notable antitumor effects in mice /
[ Shuto,S.;H.Awano;N.Shimazaki;K.Hanaoka;a.Matsuda ] / Bioorg. Med. Chem. Lett.   ScienceOn
16 Bioconversion of phosphatidylcholine to phosphatidylserine using immobilized enzyme mini-columns /
[ Yaqoob,M.;A.Nabi;M.Masoon Yasinzai ] / Process Biochem
17 Transphosphatidylation reaction of phosphatidylcholine to 4-methoxyphenol in water-immiscible organic solvents with immobilized phospholipase D /
[ Takami,M.;Y.Suzuki ] / J. Ferment. Bioeng