Browse > Article

Purification, Characterization, and Gene Cloning of Chitosanase from Bacillus cereus H-l  

Jang, Hong-Ki (School of Biotechnology and Bioengineering, Kangwon National University)
Yi, Jae-Hyoung (School of Biotechnology and Bioengineering, Kangwon National University)
Kim, Jung-Tae (School of Biotechnology and Bioengineering, Kangwon National University)
Lee, Keun-Eok (School of Biotechnology and Bioengineering, Kangwon National University)
Park, Shin-Geon (School of Biotechnology and Bioengineering, Kangwon National University)
Publication Information
Microbiology and Biotechnology Letters / v.31, no.3, 2003 , pp. 216-223 More about this Journal
Abstract
A 1.3-kb of chitosanase gene (choA) encoding 45-kDa polypeptide was cloned, expressed, and characterized from a newly isolated Bacillus cereus H-1. The chitosanase protein (ChoA) of B. cereus H-l was purified to homogeneity by ammonium sulfate precipitation and CM-sephadex column chromatography. Optimum pH was around 7, and stable pH range in the incubation at 50 C was 4-11. Optimum temperature was around 50 C, and enzyme activity was relatively stable below 45 C. ChoA showed the activities toward carboxymethyl cellulose (CMC) in addition to soluble or glycol chitosan. Based on MALDI-TOF MS analysis of purified ChoA, the entire amino acid sequence of ChoA was interpreted by database searching of previously known Bacillus chitosanases. A 1.6 kb of PCR product of corresponding chitosanase gene was obtained and its DNA sequence was determined. The deduced amino acid of choA revealed that ChoA have a 98% homology with those of Bacillus sp. No.7-M strain and Bacillus sp. KCTC0377BP. The recombinant ChoA protein was expressed in E. coli DH5$\alpha$. Deduced amino acid comparison of choA with other chitosanases suggested that it belongs to family 8 microbial endo-chitosanase with chitosanase-cellulase activity.
Keywords
Bacillus cereus; chitosanase; cellulase; gene cloning;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Thermostable chitosanase from Bacillus sp. Strain CK4: cloning and expression of the gene and characterization of the enzyme /
[ Yoon,H.G.;H.Y.Kim;Y.H.Lim;H.K.Kim;D.H.Shin;B.S.Hong;H.Y.Cho ] / Appl. Environ. Microbiol.   DOI   ScienceOn
2 Identification and production of constitutive chitosanase from Bacillus sp. HW-002 /
[ Lee, H.W.;J.W.Choi;D.P.Han;N.W.Lee;S.L.Park;D.H.Yi ] / J. Microbiol. Biotechnol.   과학기술학회마을
3 Chitosanase activity of the enzyme previously reported as β-1,3 glucanase from Bacillus circulans WL-12 /
[ Mitsutomi,M.;M.Isono;A.Uchiyama;N.Nikaidou;T.Ikegami;T.Watanabe ] / Biosci. Biotechnol. Biochem.   DOI   ScienceOn
4 Purification and some properties of novel chitosanase from Bacillus subtilis KH1 /
[ Omumasaba,C.A.;N.Yoshida;Y.Seiguchi;K.Kariva;K.Ogawa ] / J. Gen. Appl. Microbiol.   DOI   ScienceOn
5 Purification and properties of a chitosanase from Pseudomonas sp. H-14 /
[ Kazutoshi,Y.;J.Hosokawa;T.Kubo;M.Nishiyama;Y.Koba ] / Biosci. Biotech. Biochem.   DOI
6 Purification and properties o chitosanase from Pseudomonas sp. H-14 /
[ Yoshihara,K.;K.J.Hosokawa;T.Kubo;M.Nishiyama;Y.Koba ] / Biosci. Biotech. Biochem.   DOI
7 Molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium Burholderia gladioli strain CHB101 /
[ Shimosaka,M.;Y.Fukumor;X.Y.Zhang;N.J.He;R.Kodaira;M.Okazaki ] / Appl. Microbiol. Biotechnol.   DOI   ScienceOn
8 Purification and mode of action of a chitosanase from Penicillium islandicum /
[ Fenton,D.M.;E.M.Eveleigh ] / J. Gen. Microbiol.
9 Chitosanase-properties and applications: a review /
[ Somashekar,D.;R.Joseph ] / Bioresource Technol.   DOI   ScienceOn
10 Purification and characterization of chitosanase from Streptomyces griseus /
[ Ohtakara,A.;H.Ogata;Y.Taketomi;M.Mitsutomi;J.P.Zikakis(ed.) ] / Chitin, Chitosan, and Related enzymes
11 Purification and mode of action of chitosanolytic enzyme from Enterobacter sp. G-1 /
[ Yamasaki,Y.;I.Hayashi;Y.Ohta;T.Nakagawa;M.Kawamukai;H.Matsuda ] / Biosci. Biotech. Biochem.   DOI
12 Purification, characterization, and gene anlaysis of a chitosanase(choA) from Matsuebacter chitsanotbidus 3001 /
[ Park,J.K.;K.Shimono;N.Ochiai;K.Shigeru;M.Kurita;Y.Ohta;K.Tanaka;H.Matsuda;M.Kawamukai ] / J. Bacteriol.
13 Characterization, commercial production, and application of a chitosanase from Bacillus sp. KCTO03770BP /
[ Choi,Y.J.;M.J.Ryu;E.J.Kim;Y.S.Kim;Y.C.Shin ] / Proceeding of International Symposium on Utilization of Chitin and Chitosan
14 Extracellular enzyme from Myxobacter AL-1 that exhibits both β-glucanase and chitosanase activities /
[ Allan,H.A.;R.S.Wolfe ] / J. Bacteriol.
15 16S ribosomal DNA amplification for phylogenetic study /
[ Weisburg,W.G.;S.M.Barns;D.A.Pelletier;D.J.Lane ] / J. Bacteriol.
16 Biomass. Part B. Lignin,pectin and chitin /
[ Wood,W.A.;S.T.Kellogg ] / Methods Enzymol.   DOI
17 Purification and characterization of three chitosanase activities from Bacillus megaterium P1 /
[ Pellertier,A.;J.Sygush ] / Appl. Environ. Microbiol.
18 Thermostable chitosanase form Bacillus sp. Strain CK4: cloning and expression of the gene and characterization of the enzyme /
[ Yoon,H.G.;H.Y.Kim;Y.H.Lim;H.K.Kim;D.H.Shin;B.S.Hong;H.Y.Cho ] / Appl. Environ. Microbiol.   DOI   ScienceOn
19 Properties and action pattern of a chitosanase from Bacillus sp. PI-7S /
[ Seino,H.;K.Tsukuda;Y.Shimasue ] / Agric. Biol. Chem.   DOI
20 Purification and characterization of a chitosanase from Streptomyces N174 /
[ Isabelle,B.;A.Dupuy;P.Vidal. Meugebauer;R.Brzezinski ] / Appl. Microbiol. Biotechnol.
21 Chitosanase from Bacillus species /
[ Yasushi,U.;A.Ohtakara ] / Methods Enzymol.   DOI
22 Properties of chitosanase from Bacillus cereus S1 /
[ Kurakake,M.;S.Yo-u;K.Nakagawa;M.Sugihara;T. Komaki T. ] / Cur. Microbiol.   DOI   ScienceOn
23 Novel chitosanase from Streptomyces griseus HUT 6037 with transglycosylation activity /
[ Tanabe,T.;K.Morinaga;T.Fukamizo;M.Mitsutomi ] / Biosci. Biotechnol. Biochem.   DOI   ScienceOn
24 Fermentation conditions and properties of a chitosanase from Acinetobacter sp. C-17 /
[ Zhu,X.F.;X.Y.Wu;Y.Dai ] / Biosci. Biotech. Biochem.   DOI   ScienceOn
25 /
[ Japanase Society for Chitin and Chitosan ] / Application of chitin and chitosan
26 Gene cloning and biochemical analysis of thermostable chitosanase(TCH-2)from Bacillus coagulans CK108 /
[ Yoon,H.G.;K.H.Lee;H.Y.Kim;H.K.Kim;D.H.Shin;B.S.Hong;H.Y.Cho ] / Biosci. Biotechnol. Biochem.   DOI   ScienceOn
27 Action pattern of Bacillus sp. No. 7-M chitosanase on partially N-acetylated chitosan /
[ Masato,I;S.Nagae;H.Kawagishi;M.Mitsuromi;A.Ohtakara A. ] / Biosci. Biotech. Biochem.   DOI   ScienceOn
28 Purification and characterisics of chitosanase from Bacillus sp. HW-002 /
[ Lee,H.W.;J.W.Choi;D.P.Han;N.W.Lee;S.L.Park;D.H.Yi ] / J. Microbiol. Biotechnol.   과학기술학회마을