Browse > Article

Isolation of Bacillus alcalophilus AX2000 Producing Alkaling Xylanase and Its Enzyme Production  

박영서 (경원대학교 공과대학 생명공학부)
김태영 (경원대학교 공과대학 생명공학부)
Publication Information
Microbiology and Biotechnology Letters / v.31, no.2, 2003 , pp. 157-164 More about this Journal
Abstract
An alkali-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus alcaiophilus. This strain, named B. alcalophilus AX2000, was able to grow and produce xylanase optimally at pH 10.5 and $37^{\circ}C$. The maximum xylanase production was obtained when 0.5%(w/v) birchwood xylan and 0.5%(w/v) polypeptone and yeast extract were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression by glucose in the culture medium, and inhibited in the presence of high concentration of xylose. The maximum activity of xylanase was observed at pH 10.0 and $50^{\circ}C$ and the enzyme activity remained was over 80% at $60^{\circ}C$ and from pH 5.0 to 11.0.
Keywords
Bacillus alcalophilus; xylanase; alkali-tolerant; enzyme production;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Glucose effects: inducer exclusion and repression /
[ Magasanik,B.;J.R.Beckwith(ed.)D.Zipser(ed.) ] / The lactose operon
2 Molecular cloning and expression of a xylanase gene of alkalophilic Aeromonas sp. No. 212 in Escherichia coli /
[ Kudo,J.;K.Ohkoshi ] / J. Gen. Microbiol.
3 Cyclic nucleotides in procaryotes /
[ Botsford,J.L. ] / Microbiol. Rev.
4 Purification and properties of thermostable xylanase from Talaromyces byssochlamydoides YH-50 /
[ Yoshioka,H.;N.Nagato;S.Chavanich;N.Nilubol;S.Hayashida ] / Agric. Biol. Chem.
5 Isolation and enzyme production of a xylanase-producing strain, Bacillus sp. AMX-4 /
[ Yoon,K.H.;S.J.Seol;H.C.Cho;M.S.Lee;J.H.Choi;K.H.Cho ] / Kor. J. Microbiol. Biotechonol.   과학기술학회마을
6 xylanase produced by alkalophilic Bacillus no. C-59-2 /
[ Hor koshi,K.;Y.Atsukawa ] / Agric. Biol. Chem.
7 Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in Gram-positive bacteria /
[ Deuscher,J.;E.Kuster;U.Bergstedt;V.Charrier;W.Hillen ] / Mol. Microbiol.   ScienceOn
8 Phosphoenolpyruvate: carbohydrate phosphotransferase system of bacteria /
[ Postma,P.W.;J.W.Lengeler;G.R.Jacobson ] / Microbiol. Rev.
9 Note on sugar determination /
[ Somogyi,M. ] / J. Biol. Chem.
10 Wood hemicelluloses: Part /
[ Timell,T.E. ] / Carbohydr. Chem.
11 Xylanolytic enzymes from fungi and bacteria /
[ Sunna,A.;G.Antranikian ] / Crit. Rev. Biotechnol.   ScienceOn
12 Signal transduction by the bacterial phosphotransferase system /
[ Roseman,S.;N.D.Meadow ] / J. Microbiol.
13 Molecular and biotechnological aspects of xylanases /
[ Kulkarni,N.;A.Shendye;M.Rao ] / FEMS Microbiol. Rev.   ScienceOn
14 Selection and characterization of catabolite repression resistant mutant of Bacillus firmus var. alkalophilus producing cyclo-dextrin glucanotransferase /
[ Do,E.J.;H.D.Shin;C.Kim;Y.H.Lee ] / J. Microbiol. Biotechnol.   과학기술학회마을
15 Recent progress in the chemistry of wood hemicelluloses /
[ Timell,T.E. ] / Wood Sci. Technol.
16 Structural chemistry of the hemicellulose /
[ Aspinall,G.O. ] / Adv. Carbohydr. Chem.   ScienceOn
17 Xylanases: functions, properties and applications /
[ Woodward,J. ] / Top. Enzyme Ferment. Biotechnol.
18 The role of phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system. in the regulation of carbon metabolism in Gram-positive bacteria /
[ Reizer,J.;A.H.Romano;J.Deutscher ] / J. Cell. Biochem.
19 Bleaching of Kraft pulp with commercial xylanase /
[ Jose,H.B.;F. Fava-De-Moraes;G.M.Zanin ] / Appl. Biochem. Biotechnol.
20 Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6 /
[ Khasin,A.;I.Alchanati;Y.Shoham ] / Appl. Environ. Microbiol.
21 Purification and properties of endoxylanase produced by Bacillus pumilus /
[ Panbangred,W.;A.Shinmyo;S.Kinoshita;H.Okada ] / Agric. Biol. Chem.
22 Production of xylanase by Aspergillus awamori on synthetic medium in shake flask cultures /
[ Siedenberg,D.;S.R.Gerlach;K.Schugerl;M.L.F.Giuseppin;J.Hunik ] / Process Biochem.
23 Genes controlling xylan utilization by Bacillus subtilis /
[ Roncero,M.I. ] / J. Bacteriol.
24 Thermophilic alkaline xylanase from newly isolated alkalophilic and thermophilc Bacillus sp. strain TAR-1 /
[ Nakamura,S.;R.Nakai;K.Wajabatacgu;Y.Ishiguro;R.Ano;K.Horikoshi ] / Biosci. Biotechnol. Biochem.
25 Purification and characterization of a thermophilic xylanase from the brown-rot fungus Gleophyllum trabeum /
[ Ritschkoff,A.C.;J.Buchert;L.Vikari ] / J. Biotechnol.   ScienceOn
26 Identification of a co-repressor binding site in catabolite control protein CcpA. /
[ Kraus,A.;E. Kuster;A.Wagner;K.Hoffmann;W.Hillen ] / Mol. Microbiol.   ScienceOn
27 Xylanase induction by L-sorbose in a fungus Trichoderma reesei PC-3-7 /
[ Xu,J.;Nogawa, H. Okada;Y.Morikawa ] / Biosci. Biotechnol. Biochem.
28 Microbial xylanolytic systems /
[ Biely,P. ] / Trends Biotechnol.   ScienceOn
29 CcpA and HPr(ser-P): Mediators of catabolite repression in Bacillus subtilis /
[ Chauvaux,S. ] / Res. Microbiol.   ScienceOn
30 A multiplicity of potential carbon catabolite repression mechanisms in prokaryotic and eukaryotic microorganisms /
[ Saier,M.H.Jr. ] / The New Biologist
31 Studies on xylan degrading enzymes. I. Purification and characterization of endo-1,4-β-xylanase from Aspergillus niger str. 14. /
[ Gorbacheba,I.V.;N.A.Rodionova ] / Biochim. Biophys. Acta   ScienceOn
32 Induction of mannanase, xylanase and endoglucanase activities in Sclerotium rolfsii /
[ Sachslehner,A.;B.Nidetzky;K.D.Kulbe;D.Haltrich ] / Appl. Environ. Microbiol
33 Cis sequences involved in modulating expression of Bacillus licheniformis amyL in Bacillus subtilis: Effect of sporulation mutations and catabolite repression resistance matations on expression /
[ Laoide B.M.;D.J.J.McConnell ] / J. Bacteriol.
34 Multiplicity of β-1,4-xylanase in microorganism: functions and applications /
[ Wong,K.K.Y.;L.U.L.Tan;J.N.Saddler ] / Microbiol. Rev.
35 Production and characterization of thermostable xylanase and pectinase from a Streptomyces sp. QG-11-3 /
[ Beg,O.K.;B.Bhushan;M.Kapoor;G.S.Hoondal ] / J. Ind. Microbiol. Biotechnol.   ScienceOn
36 The bacterial phosphoenolpyruvate: glycose phosphotransferase system /
[ Meadow,N.D.;D.K.Fox;S.Roseman ] / Annu. Rev. Biochem.   ScienceOn
37 Multiple mechanisms controlling carbon metabolism in bacteria /
[ Saier,M.H.Jr. ] / Biotechnol. Bioeng.   ScienceOn
38 Applications of hemicellulases in the food, feed, and pulp and paper industries /
[ Wong,K.K.Y.;J.N.Saddler;Coughlen,P.P.(ed.);G.P.Hazlewood(ed.) ] / Hemicellulose and Hemicellulases