Production of Enantioselective Lipase from Acinetobacter sp. SY-01

Lipase from Acinetobacter sp. SY-01 plays an important role enzyme that products chiral drug. We investigated optimum condition for mass production of Acinetobacter sp. SY-01 lipase. Addition of among the different oils to medium. olive oil was optimal for enzyme production. When 0.2% olive oil was added as a carbon source, the production of lipase was increased to a maximum. The optimum pH and temperature were pH 7 and $30^{\circ}C$. In the presence of $Fe^{2＋}$ and $Ca^{2＋}$, the lipase activity was dramatically enhanced by 280% and 160%, respectively. SY-01 lipase was stable in the most of the DMSO among organic solvents. The addition of triton-X 100 increased the SY-01 lipase by 100-fold. The optimum composition of medium for production of the enzyme was 0.8% yeast extract, 0.2% olive oil, 0.4% triton X-100＋40% DMSO. 0.1% $NH_4Cl$, 0.4% $K_2HPO_4$ 3.9% $NaH_2PO_4$, 0.03% $CaCl_22H_2O$, 0.01% $FeSO_4$$7H_2O$(pH 7.0).

Acinetobacter sp. SY-01로부터 Enantioselective Lipase의 생산

Acinetobacter sp. SY-01가 생산하는 lipase는 소염진통제로 쓰이는 azolerP 의약품 중간체인 두 enantiomer를 가진 rcemate에 대해 enantioselectivity를 가지는 효소로써 chiral drug를 생산하는데 있어 중요한 역할을 수행한다. Acinetobacter sp. SY-01 lipase 효소의 다량 생산을 위한 여러 가지 최적 조건을 조사하였다. 탄소원으로는 여러 가지 천연 오일을 이용하여 실험한 결과 0.2% olive oil이 가장 좋았으며, 최적 온도와 pH는 30도와 pH 7이였다. EH한 금속이온과 유기용매 안정성에 있어서는 $Fe^{2＋}$ 과 $Ca^{2＋}$ DMSO가 좋은 효과를 나타냇었다. 특히 DMSO는 유기용매가 첨가되지 않았을 때보다 약 2.5배 높은 효소 활성을 나타내었다. 계면활성제 이용에 있어서는 tritom X-100을 이용했을 때 좋은 효과를 나타냈다. Acinetobacter sp. SY-01가 생산하는 lipase 효소의 최적 배지 조성은 0.8% yeast extract, 0.2% olive oil 0.4% triton X-100, 40% DMSO, 0.1 % $NH_4$Cl% 0.4%$K_2HPO_4$ 3.9% $NaH_2PO_4$ 0.03% $CaCl_22H_2O$ 0.01% $FeSO_4$$7H_2O$(pH 7.0)이다.