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Characteristics of Hemolysin in Mosquitocidal Bacillus thuringiensis strain 21-2  

김광현 (동의대학교 미생물학과)
김위종 ((주)고려식료)
김영희 (동의대학교 미생물학과)
김병우 (동의대학교 미생물학과)
Publication Information
Microbiology and Biotechnology Letters / v.30, no.3, 2002 , pp. 230-234 More about this Journal
Abstract
To describe characteristics of a hemolysin in mosquitocidal Bacillus thuringiensis subsp. gyangiensis strain 21-2, Escherichia coli HB101 was transformed with a gene encoding hemolysin in the strain 21-2. Transformant 47 con-tained 2.5 kb DNA was selected by ELISA, immunoblot and DNA electrophoresis. Transformant 47-5 was recon-structed after digestion of the 2.5 kb DNA with Hind m. Transformant 47-5 contained 1.8 kb DNA and expressed 23 kDa Protein which had mosquitocidal activity to Aedes aegypti. The 23 kDa Protein itself in vitro didn't show hemolytic activity on human erythrocytes, but the protein had the activity after proteinase K treatment.
Keywords
Bacillus thuringiensis; cyt gene; hemolytic; mosquitocidal activity; delta-endotoxin;
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