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Isolation and Enzyme Production of a Xylanase-producing Strain, Bacillus sp. AMX-4.  

윤기홍 (우송대학교 식품생명과학부)
설숙자 (우송대학교 식품생명과학부)
조효찬 (우송대학교 식품생명과학부)
이미성 (씨티씨바이오 중앙연구소)
최준호 (씨티씨바이오 중앙연구소)
조기행 (씨티씨바이오 중앙연구소)
Publication Information
Microbiology and Biotechnology Letters / v.30, no.2, 2002 , pp. 123-128 More about this Journal
Abstract
A bacterium producing the extracellular xylanase was isolated from soil and has been identified as a Bacillus sp. strain. The isolate, named Bacillus sp. AMX-4, was shown to be similar to B. subtilis strain on the basis of its chemical compositions. The xylanase of culture supernatant was most active at 50℃ and pH 6.0. The additional carbon sources including monosaccharides, disaccharides, wheat bran, and rice straw increased the enzyme productivity. Especially, the maximum xylanase productivity was reached 29.2 units/ml in LB medium supplemented with 1.5% (w/v) xylose, which was 16-folds more than that in LB medium. As the results of investigating the effects of xylose on cell growth and xylanase productivity of Bacillus sp. AMX-4, increase of xylanase production was owing to the induction of xylanase biosynthesis. It was also found that the enzyme production was in association with the growth of Bacillus sp. AMX-4.
Keywords
Bacillus; identification; xylose; xylanase production;
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Times Cited By KSCI : 3  (Citation Analysis)
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