Browse > Article

Structural Analysis of the Antifungal Antibiotic from Bacillus sp. YJ-63.  

정영기 (동의대학교 미생물학과)
신영준 (동의대학교 미생물학과)
정명주 (경성대학교 생물학과)
주우홍 (창원대학교 생물학과)
최재수 (부경대학교 식품생명공학부)
Publication Information
Microbiology and Biotechnology Letters / v.30, no.1, 2002 , pp. 21-25 More about this Journal
Abstract
Structural analysis was performed by the $^1$H-NMR, $^{13}$ C-NMR, amino acid composition analysis and FAB-mass. The instrumental analysis represented that the potential antifungal antibiotic belonged to the iturin E group antibiotic, consisting of 7 $\alpha$-amino acid residues and a collection of $\beta$-amino acid with aliphatic side chain. Compared to the Iturin E group, notably, the potent antifungal antibiotic from Bacillus sp. YJ-63 carried longer $\beta$-amino acid side chain. In conclusion, these findings identified a potential antibiotic, which contained a stable cyclopeptide structure with long $\beta$-amino acid side chain.
Keywords
Antifungal agent; Antibiotic; Thermostable antibiotic;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Besson, F., M. L. Hourdou, and G. Michel. 1990. Studieson the biosynthesis of iturin, an antibiotic of Bacillus subtilis, and a lipopeptide containing beta-hydroxy fatty acids. Biochim. Biophys. Acta. 1032: 101-106.
2 Cook, R. J. 1985.Biological control of plant pathogens: Theory to application. Phytopathol. 75: 25-28.   DOI
3 Davison, J. 1988. Plant beneficial bacteria. Biotechnol. 6: 282-286.
4 Emmer, G., N. S. Ryder, and M. A. Grassberger. 1985. Synthesis of new polyoxin derivatives and their activity against chitin synthase from Candida albicans. J. Med. Chem. 28: 278-281.
5 Leong,J. 1986. Siderophores : Their biochemistry and possible role in biocontrol of plant pathogens. Ann. Rev. Phtopathol. 24: 187-209.
6 Shin, Y. J., M. J. Jung, and Y. K. Jeong. 2000. Optimization of the productionof a thermostable antifungal antibiotic. Kor. J. Biotechnol. Bioeng. 15: 584-588.
7 Zhang,D. and M. J. Miller. 1999. Polyoxins and nikkomycins: progress in synthetic and biological studies. Curro Pharm. Des. 5: 73-99.
8 Yonehara, H., H. Seto, S. Aizawa, T. Hidaka, and A Shimazu. 1968. The detoxin complex, selective antagonists of blasticidin S. J. Antibiot. 21: 369-370.
9 Corbell, N. and J. E. Loper. 1995.A global regulatorof secondary metabolite production in Pseudomonas fluorescens Pf-5. J. Bacteriol. 177: 6230-6236.
10 Peypoux,F., M. T. Pommier, D. Marion,M. Ptak, B. C. Das, and G. Michel. 1986. Revised structure of mycosubtilin, a peptidolipid antibiotic from Bacillus subtilis. J. Antibiot. 39: 636-641.
11 Maget-Dana, R. and F. Peypoux. 1994.Iturins, a special class of pore-forming lipopeptides: biological and physicochemical properties. Toxicology, 87: 151-174.
12 Eshita, S. M., N. H. Roberto, J. M. Beale, B. M. Mamiya, and R. F. Workman. 1995.Bacillomycin Lc, a new antibiotic of the iturin group: isolations, structures, and antifungal activities of the congeners. J. Antibiot. 48: 1240-1247.
13 Shin, Y. J., M. J. Jung, J. U. Park, W. H. Joo, and Y. K. Jeong. 2000. Isolation and identification of a bacterium producing antifungal antibiotic. J. Kor. Soc. Food Sci. Nutr. 29: 832-836.
14 Hotta, K. 1996. Mechanism of multiple aminoglycoside resistance of kasugamycin -producing Streptomyces kasugaensis MB273 : involvement of two types of acetyltransferases in resistance to astromicin groupantibiotics. J. Antibiot. 49: 682-688.
15 Tenoux, I., F. Besson, and G. Michel. 1991. Studies on the antifungal antibiotics: bacillomycin D and bacillomycin D methylester. Microbios. 67: 187-193.
16 Duffy, B. K. and G. Defago. 1999. Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Appl. Environ. Microbiol. 65: 2429-2438.
17 Uramoto,M, C. J. Kim, K. Shin-Ya, H. Kusakabe, K. Isono, D. R. Phillips, and J. A McCloskey. 1991. Isolation and characterization of phosrnidosine. A new antifungal nucleotide antibiotic. J. Antibiot. 44: 375-381.
18 Miyamoto, Y. and S. Ogawa. 1992. Total synthesis of (+)validamycin H. Carbohydn Res. 223: 299-301.
19 Besson, F. and G. Michel. 1990. Mycosubtilins B and C: minor antibiotics from mycosubtilin-producer Bacillus subtilis. Microbios. 62: 93-99.   PUBMED
20 Kirner, S., P. E. Hammer, D. S. Hill, A Altmann, I. Fischer, L. J. Weislo, M. Lanahan, K. H. van Pee, and J. M. Ligon. 1998. Functions encoded by pyrrolnitrin biosynthetic genes from Pseudomonas fluorescens. J. Bacteriol. 180: 19391943.
21 Larsen, S. H., D. M. Berry,1. W. Paschal, and J. M. Gilliam. 1989. 5-Hydroxymethyl blasticidinS and blasticidinS from Streptomyces setonii culture A 83094.J. Antibiot. 42: 470471.
22 Asano, N., Y. Kameda, K. Matsui, S. Horii, H. Fukase, and H. Validamycin. 1990.A new pseudo-tetrasaccharide antibiotic. J. Antibiot. 43: 1039-1041.
23 Bidlingmeyer, B. A, S. A Cohen, and T. L. Tarvin. 1984. Rapid analysis of amino acids using pre-column derivation. J. Chromagraphy. 366: 93-104.
24 Kuhnt, M., F. Bitseh,M. Ponelle, J. J. Sanglier, Y. Wang, and B. Wolff. 1998. Microbial conversion products of leptomycin B. Appl. Environ. Microbiol. 64: 714-720.