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http://dx.doi.org/10.7845/kjm.2018.8079

Cellulose degrading basidiomycetes yeast isolated from the gut of grasshopper in Korea  

Kim, Ju-Young (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
Jang, Jun Hwee (College of Agricultural and Life Sciences, Kyungpook National University)
Park, Ji-Hyun (Institute for Healthcare and Life Science, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Jung, Hee-Young (College of Agricultural and Life Sciences, Kyungpook National University)
Park, Jong-Seok (School of Biological Sciences, College of Natural Sciences, Chungbuk National University)
Cho, Sung-Jin (School of Biological Sciences, College of Natural Sciences, Chungbuk National University)
Lee, Hoon Bok (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
Limtong, Savitree (Department of Microbiology, Faculty of Science, Kasetsart University)
Subramani, Gayathri (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
Sung, Gi-Ho (Institute for Healthcare and Life Science, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
Publication Information
Korean Journal of Microbiology / v.54, no.4, 2018 , pp. 362-368 More about this Journal
Abstract
Grasshoppers play vital role in the digestion of photosynthetically fixed carbons. With the aid of intestinal microflora, the grasshopper can degrade leaves constituents such as cellulose and hemicellulose. The purpose of this study was to examine cellulolytic yeast isolates from the gut of grasshoppers collected in Gyeonggi Province, South Korea. Among the yeast isolates, ON2, ON17 (two strains), and ON6 (one strain) showed positive cellulolytic activity in the CMC-plate assay. The sequence analyses of D1/D2 domains of the large subunit rDNA gene and the internal transcribed spacer (ITS) regions revealed that the strains ON2 and ON17 were most closely related to Papiliotrema aspenensis CBS $13867^T$ (100%, sequence similarity in D1/D2 domains; 99.4% sequence similarity in ITS) and strain ON6 related to Saitozyma flava (100% in D1/D2 domains; 99.0% in ITS). All these three yeast strains are capable of degrading cellulose; therefore, the members of endosymbiotic yeasts may produce their own enzymes for carbohydrate degradation and convert mobilized sugar monomers to volatile fatty acids. Thus, the endosymbiotic yeast strains ON2, ON17 (represents the genus Papilioterma) and ON6 (Saitozyma) belonging to the family Tremellomycetes, are unreported strains in Korea.
Keywords
Papiliotrema sp.; Seitozyma sp.; rRNA; grasshopper; yeast;
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