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http://dx.doi.org/10.7845/kjm.2018.8021

Resistance genes in high-level streptomycin resistant Escherichia coli isolated from shellfish  

Lim, Chan Seok (Department of Biology, Sunchon National University)
Lee, Young Sun (Department of Biology, Sunchon National University)
Kahng, Hyung-Yeel (Department of Environmental Education, Sunchon National University)
Ahn, Samyoung (Department of Environmental Education, Sunchon National University)
Jung, Jae Sung (Department of Biology, Sunchon National University)
Publication Information
Korean Journal of Microbiology / v.54, no.3, 2018 , pp. 228-236 More about this Journal
Abstract
The aim of this study was to investigate the distribution of resistance genes in high-level streptomycin resistant Escherichia coli isolated from shellfish collected between April 2015 and March 2016 in Korea. From the 269 E. coli isolates obtained from shellfish samples, a total of 40 streptomycin-resistant isolates with MICs of > $1,024{\mu}g/ml$ were screened and the prevalence of streptomycin resistance determinants was analyzed by PCR. Among the isolates, strA-strB gene structure (77.5%) was the most frequent streptomycin resistance determinant, followed by aadA (30.0%). Six isolates (15.0%) simultaneously contained aadA and strA-strB determinants, whereas three of the isolates (7.5%) did not contain both resistance determinants examined in this work. The difference of MICs between the isolates having the same resistance gene was elucidated by real-time PCR results. The copy number of resistance genes differed considerably among the isolates, which solely harbored an aadA or strA-strB and showed different MICs.
Keywords
Escherichia coli; aadA; resistance gene; strA-strB; streptomycin;
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