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http://dx.doi.org/10.7845/kjm.2018.8035

Studies of cold resistant glycine betaine effect on cold sensitive Bacillus subtilis mutant strains  

Kim, Do Hyung (Department of Life Science and Technology, Pai Chai University)
Lee, Sang Soo (Department of Life Science and Technology, Pai Chai University)
Publication Information
Korean Journal of Microbiology / v.54, no.3, 2018 , pp. 200-207 More about this Journal
Abstract
At high salt concentration, glycine betaine is transported into Bacillus subtilis and growing rate of the cell is not suppressed. Also according to recent studies, cell growth is maintained normal growth rate at low temperature. Low temperature results in a stress response of Bacillus subtilis that is characterized by strong repression of major metabolic activities such as translation machinery and membrane transport. In this regards, genes showing cold sensitive phenotype are cold-induced DEAD box RNA helicases (ydbR, yqfR) and fatty acid desaturases (bkdR, des). Therefore to understand the effect of glycine betaine on cold growth of Bacillus subtilis, we investigated the effect of glycine betaine on growth rate of these deletion mutants showing cold sensitive phenotype. Glycine betaine strongly stimulated growth of wild type Bacillus subtilis JH642 and deletion mutants of ydbR and yqfR at $20^{\circ}C$ (190~686 min $T_d$ difference). On the other hands, glycine betaine does not show growth promoting effects on deletion mutants of bkdR, and des at cold conditions. Same cold protectant growth results were shown with the precursor choline instead of glycine betaine. We investigated the effects of detergents on the cell membrane in bkdR and des deficient strains associated with cell membrane. It was identified that bkdR deficient strain shows retarded growth with detergent such as Triton X-100 or N-lauryl sarcosine compared with wild type cell. Thus, it is possible that deletion mutation of bkdR modifies membrane structure and effects on transport of glycine betaine.
Keywords
Bacillus subtilis; choline; DEAD box RNA helicase; glycine betaine; $T_d$;
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1 Strocchi M, Ferrer M, Timmis KN, and Golyshin PN. 2006. Low temperature-induced systems failure in Escherichia coli: insights from rescue by cold-adapted chaperones. Proteomics 6, 193-206.   DOI
2 Kaan T, Homuth G, Mader U, Bandow J, and Schweder T. 2002. Genome-wide transcriptional profiling of the Bacillus subtilis cold-shock response. Microbiology 148, 3441-3455.   DOI
3 Kappes RM, Kempf B, Kneip S, Boch J, Gade J, Meier-Wagner J, and Bremer E. 1999. Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis. Mol. Microbiol. 32, 203-216.   DOI
4 Whatmore AM, Chudek JA, and Reed RH. 1990. The effects of osmotic up shock on the intracellular solute pools of Bacillus subtilis. J. Gen. Microbiol. 136, 2527-2535.   DOI
5 Ziegler C, Bremer E, and Kramer R. 2010. The BCCT family of carriers: from physiology to crystal structure. Mol. Microbiol. 78, 13-34.
6 Budde I, Steil L, Scharf C, Volker U, and Bremer E. 2006. Adaptation of Bacillus subtilis to growth at low temperature: a combined transcriptomic and proteomic appraisal. Microbiology 152, 831-853.   DOI
7 Chen TH and Murata N. 2011. Glycinebetaine protects plants against abiotic stress: mechanisms and biotechnological applications. Plant Cell Environ. 34, 1-20.   DOI
8 Cybulski LE, Albanesi D, Mansilla MC, Altabe S, Aguilar PS, and de Mendoza D. 2002. Mechanism of membrane fluidity optimization: isothermal control of the Bacillus subtilis acyl-lipid desaturase. Mol. Microbiol. 45, 379-388.
9 Debarbouille M, Gardan R, Arnaud M, and Rapoport G. 1999. Role of BkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis. J. Bacteriol. 181, 2059-2066.
10 Altabe SG, Aguilar P, Caballero GM, and de Mendoza D. 2003. The Bacillus subtilis acyl lipid desaturase is a ${\Delta}5$ desaturase. J. Bacteriol. 185, 3228-3231.   DOI
11 Beckering CL, Steil L, Weber MHW, Volker U, and Marahiel MA. 2002. Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis. J. Bacteriol. 184, 6395-6402.   DOI
12 Boch J, Kempf B, and Bremer E. 1994. Osmoregulation in Bacillus subtilis: synthesis of the osmoprotectant glycine betaine from exogenously provided choline. J. Bacteriol. 176, 5364-5371.   DOI
13 Boch J, Kempf B, Schmid R, and Bremer E. 1996. Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes. J. Bacteriol. 178, 5121-5129.   DOI
14 Bremer E. 2002. Adaptation to changing osmolarity, pp. 385-391. In Sonenshein AL, Hoch JA, and Losick R. (eds.), Bacillus subtilis and its closest relatives. ASM Press, Washington, DC, USA.
15 Kim DH and Lee SS. 2018. Cold shock sensitive growth of Bacillus subtilis mutants deleted for genes involved in fatty acid synthesis. Korean J. Microbiol. 54, 9-17.
16 Bremer E and Kramer R. 2000. Coping with osmotic challenges: osmoregulation through accumulation and release of compatible solutes in bacteria, pp. 79-97. In Storz G and Hengge-Aronis R. (eds.), Bacterial stress responses. ASM Press, Washington, DC, USA.
17 Brigulla M, Hoffmann T, Krisp A, Volker A, Bremer E, and Volker U. 2003. Chill induction of the SigB-dependent general stress response in Bacillus subtilis and its contribution to low-temperature adaptation. J. Bacteriol. 185, 4305-4314.   DOI
18 Brill J, Hoffmann T, Bleisteiner M, and Bremer E. 2011a. Osmotically controlled synthesis of the compatible solute proline is critical for cellular defense of Bacillus subtilis against high osmolarity. J. Bacteriol. 193, 5335-5346.   DOI
19 Ahyayauch H, Larijani B, Alonso A, and Goni FM. 2006. Detergent solubilization of phosphatidylcholine bilayers in the fluid state: influence of the acyl chain structure. Biochim. Biophys. Acta 1758, 190-196.   DOI
20 Kempf B and Bremer E. 1998. Uptake and synthesis of compatible solutes as microbial stress responses to high osmolality environments. Arch. Microbiol. 170, 319-330.   DOI
21 Shivaji S and Prakash JS. 2010. How do bacteria sense and respond to low temperature? Arch. Microbiol. 192, 85-95.   DOI
22 Nau-Wagner G, Opper D, Rolbetzki A, Boch J, Kempf B, Hoffmann T, and Bremer E. 2012. Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaineresponsive GbsR repressor. J. Bacteriol. 194, 2703-2714.   DOI
23 Oh EH and Lee SS. 2010. Cold sensitive growth of deletion mutants of DEAD-box RNA helicase genes in Bacillus subtilis. Korean J. Microbiol. 46, 233-239.
24 Rodrigues DF and Tiedje JM. 2008. Coping with our cold planet. Appl. Environ. Microbiol. 74, 1677-1686.   DOI
25 Brill J, Hoffmann T, Putzer H, and Bremer E. 2011b. T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis. Microbiology 157, 977-987.   DOI
26 Feller G and Gerday C. 2003. Psychrophilic enzymes: hot topics in cold adaptation. Nat. Rev. Microbiol. 1, 200-208.   DOI
27 Hoffmann T and Bremer E. 2011. Protection of Bacillus subtilis against cold stress via compatible-solute acquisition. J. Bacteriol. 193, 1552-1562.   DOI
28 Holtmann G, Bakker EP, Uozumi N, and Bremer E. 2003. KtrAB and KtrCD: two $K^+$ uptake systems in Bacillus subtilis and their role in adaptation to hypertonicity. J. Bacteriol. 185, 1289-1298.   DOI
29 Holtmann G and Bremer E. 2004. Thermoprotection of Bacillus subtilis by exogenously provided glycine betaine and structurally related compatible solutes: involvement of Opu transporters. J. Bacteriol. 186, 1683-1693.   DOI
30 Hunger K, Beckering CL, Wiegeshoff F, Graumann PL, and Marahiel MA. 2006. Cold-induced putative DEAD-box RNA helicase CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus sutilis. J. Bacteriol. 188, 240-248.   DOI