Browse > Article
http://dx.doi.org/10.7845/kjm.2018.8020

Chlorella virus-mediated disruption of microalgal cell wall for biodiesel production  

Kim, Soojin (Department of New Drug Discovery & Development, Graduate School of New Drug Discovery & Development, Chungnam National University)
Kim, Yeon-Soo (Department of New Drug Discovery & Development, Graduate School of New Drug Discovery & Development, Chungnam National University)
Publication Information
Korean Journal of Microbiology / v.54, no.2, 2018 , pp. 140-145 More about this Journal
Abstract
The most energy-intensive processes in lipids extraction were the disruption of the cell wall of microalgae. Here, we tried to extract lipids through lysis using virus-infecting microalgae, to compare with those by the other two methods using microwave or ultrasonication. The lipids yield using viral infection was not significantly different from those using ultrasonication and microwave oven. This suggests that the same amount of lipids can be obtained with low energy and costs, as well as that microalgal lipids extraction by chlorella virus infection might provide the price competitiveness in biodiesel production even if it will be applied to mass production facilities.
Keywords
biodiesel; Chlorella; Chlorella virus PBCV-1; lipids extraction; virus-mediated lysis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Gunerken, E., D'Hondt, E., Eppink, M.H., Garcia-Gonzalez, L., Elst, K., and Wijffels, R.H. 2015. Cell disruption for microalgae biorefineries. Biotechnol. Adv. 33, 243-260.   DOI
2 Kim, D.Y., Vijayan, D., Praveenkumar, R., Han, J.I., Lee, K., Park, J.Y., Chang, W.S., Lee, J.S., and Oh, Y.K. 2016. Cell-wall disruption and lipid/astaxanthin extraction from microalgae: Chlorella and Haematococcus. Bioresour. Technol. 199, 300-310.   DOI
3 Chisti, Y. 2013. Constraints to commercialization of algal fuels. J. Biotechnol. 167, 201-214.   DOI
4 Lee, J.Y., Yoo, C., Jun, S.Y., Ahn, C.Y., and Oh, H.M. 2010. Comparison of several methods for effective lipid extraction from microalgae. Bioresour. Technol. 101 Suppl 1, S75-S77.   DOI
5 Michalak, I. and Chojnacka, K. 2014. Algal extracts: Technology and advances. Eng. Life Sci. 14, 581-591.   DOI
6 Piasecka, A., Krzeminska, I., and Tys, J. 2014. Physical methods of microalgal biomass pretreatment. Int. Agrophys. 28, 341-348.   DOI
7 Rowe, J.M., Jeanniard, A., Gurnon, J.R., Xia, Y., Dunigan, D.D., Van Etten, J.L., and Blanc, G. 2014. Global analysis of Chlorella variabilis NC64A mRNA profiles during the early phase of Paramecium bursaria chlorella virus-1 infection. PLoS One 9, e90988.   DOI
8 Safi, C., Zebib, B., Merah, O., Pontalier, P.Y., and Vaca-Garcia, C. 2014. Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renew. Sustain. Energ. Rev. 35, 265-278.   DOI
9 Skrdla, M., Burbank, D., Xia, Y., Meints, R., and Van Etten, J.L. 1984. Structural proteins and lipids in a virus PBCV-1 which replicates in a Chlorella-like alga. Virology 135, 308-315.   DOI
10 Sun, L., Adams, B., Gurnon, J.R., Ye, Y., and Van Etten, J.L. 1999. Characterization of two chitinase genes and one chitosanase gene encoded by Chlorella virus PBCV-1. Virology 263, 376-387.   DOI
11 Yamada, T., Onimatsu, H., and Van Etten, J.L. 2006. Chlorella viruses. Adv. Virus Res. 66, 293-336.
12 Van Etten, J.L., Burbank, D., Kuczmarski, D., and Meints, R. 1983a. Virus infection of culturable Chlorella-like algae and development of a plaque assay. Science 25, 994-996.
13 Van Etten, J.L., Burbank, D., Xia, Y., and Meints, R. 1983b. Growth cycle of a virus PBCV-1 that infects Chlorella-like algae. Virology 126, 117-125.   DOI
14 Wang, B., Li, Y., Wu, N., and Lan, C.Q. 2008. $CO_2$ bio-mitigation using microalgae. Appl. Microbiol. Biotechnol. 79, 707-718.   DOI
15 Bligh, E.G. and Dyer, W.J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917.   DOI
16 Brennan, L. and Owende, P. 2010. Biofuels from microalgae-A review of technologies for production, processing, and extractions of biofuels and co-products. Renew. Sustsain. Energ. Rev. 14, 557-577.   DOI
17 Bubeck, J.A. and Pfitzner, A.J. 2005. Isolation and characterization of a new type of chlorovirus that infects an endosymbiotic Chlorella strain of the heliozoon Acanthocystis turfacea. J. Gen. Virol. 86, 2871-2877.   DOI
18 Chisti, Y. 2007. Biodiesel from microalgae. Biotechnol. Adv. 25, 294-306.   DOI
19 Chisti, Y. and Yan, J. 2011. Energy from algae: Current status and future trends. Appl. Energ. 88, 3277-3279.   DOI
20 Cho, H., Park, H., Kim, J., and Choi, T. 2002. Isolation and characterization of Chlorella viruses from freshwater sources in Korea. Mol. Cells 14, 168-176.