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http://dx.doi.org/10.7845/kjm.2017.7015

Decolorization of dyes by a purified laccase from Coprinus comatus  

Kim, Su Yeon (Department of Biochemistry, Kangwon National University)
Choi, Ji Young (Department of Biochemistry, Kangwon National University)
Choi, Hyoung T. (Department of Biochemistry, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.53, no.2, 2017 , pp. 79-82 More about this Journal
Abstract
An inky cap, Coprinus comatus synthesizes and secretes a laccase in the liquid yeast extract peptone dextrose medium. We have successfully purified the enzyme through the ion-exchange chromatography and the preparative gel electrophoresis. The estimated molecular weight was 67 kDa by the SDS-PAGE analysis. Optimum pH was pH 4.3 and optimum temperature was $25^{\circ}C$. The Km value was 0.45 mM and the Vmax was 0.0132 OD/min/unit for o-tolidine. Purified laccase showed 49.3% decolorizing activity against remazol brilliant blue R (RBBR) and 41.6% decolorizing activity against Poly R-478 after 12 h incubation.
Keywords
Coprinus comatus; dye decolorization; laccase;
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Times Cited By KSCI : 1  (Citation Analysis)
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