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http://dx.doi.org/10.7845/kjm.2016.6046

Effects of Cdc31, a component of TREX-2 complex, on growth and mRNA export in fission yeast  

Koh, Eun-Jin (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University)
Yoon, Jin Ho (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University)
Publication Information
Korean Journal of Microbiology / v.52, no.3, 2016 , pp. 383-387 More about this Journal
Abstract
In fission yeast, Schizosaccharomyces pombe, the cdc31 gene encodes a member of the conserved $Ca^{2+}$-binding centrin/CDC31 family, which is a component of spindle pole body. Here, we demonstrate that the S. pombe cdc31p is also a component of TREX-2 complex, which influences mRNA export from the nucleus to the cytoplasm. Repression of the cdc31 gene expression caused growth defect with accumulation of $poly(A)^+$ RNA in the nucleus. On the other hand, over-expression of cdc31 exhibited no defects of both growth and bulk mRNA export, but showed somewhat longer cell morphology. Yeast two-hybrid analysis showed that Cdc31 interacted with Sac3 and Pci2, the subunits of TREX-2 complex. These results suggest that S. pombe Cdc31 is also involved in mRNA export as a component of TREX-2 complex.
Keywords
cdc31; fission yeast S. pombe; mRNA export; TREX-2 complex;
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