[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.7845/kjm.2016.6026

Inhibition of cell wall synthesis in Cryptococcus neoformans and decrease of skin allergy induced with Alternaria alternata in mouse model by a chitinase from an inky cap

Kang, Yuri (Department of Biochemistry, Kangwon National University)
Choi, Hyoung T. (Department of Biochemistry, Kangwon National University)

Publication Information

Korean Journal of Microbiology / v.52, no.2, 2016 , pp. 226-229 More about this Journal

Abstract

The growth of two different fungal species, human pathogenic Cryptococcus neoformans and plant pathogenic Alternaria alternata were inhibited by a chitinase (Chi2) expressed in the autolysing tissue of Coprinellus congregatus. The cell wall thickness was reduced (up to 32%) in C. neoformans compared with that of normal cell, and polysaccharide fibers located outside of the cell wall were also severely removed. The hyphal growth of A. alternata on agar plate was stopped by the enzyme. The allergic inflammation induced by A. alternata was reduced by the enzyme reaction when compared with untreated control in a mouse model.

Keywords

Alternaria alternata; Cryptococcus neoformans; allergy; chitinase;

Citations & Related Records

Times Cited By KSCI : 3 (Citation Analysis)

Reference
Cited By KSCI

1	Amian, A.A., Papenbrock, J., Jacobsen, H.J., and Hassan, F. 2011. Enhancing transgenic pea (Pisum sativum L.) resistance against fungal diseases through stacking of two antifungal genes (chitinase and glucanase). GM Crops 2, 104-109. DOI
2	Arora, S., Hernandez, Y., Erb-Downward, J.R., McDonald, R.A., Toews, G.B., and Huffnagle, G.B. 2005. Role of IFN- ${\gamma}$ in regulating T2 immunity and the development of alternatively activated macrophages during allergic bronchopulmonary mycosis. J. Immunol. 174, 6346-6356. DOI
3	Baker, L.G., Specht, C.A., and Lodge, J.K. 2011. Cell wall chitosan is necessary for virulence in the opportunistic pathogen Cryptococcus neoformans. Eukaryot. Cell 10, 1364-1368.
4	Brito, F.F., Alonso, A.M., Carnes, J., Martin-Martin, R., Fernandez-Caldas, E., Galindo, P.A., Alfaya, T., and Amo-Salas, M. 2012. Correlation between Alt a1 levels and clinical symptoms in Alternaria alternata-Monosensitized patients. J. Investig. Allergol. Clin. Immunol. 22, 154-159.
5	Choi, H.T. and Cho, C.W. 2005. Ultrastructural studies on the autolysis of Coprinellus con-gregatus. Korean J. Microbiol. 41, 312-315.
6	Distefano, G., Malfa, S.L., Vitale, A., Lorito, M., Deng, Z., and Gentile, A. 2008. Defence-related gene expression in transgenic lemon plants producing an antimicrobial Trichoderma harzianum endochitinase during fungal infection. Transgenic Res. 17, 873-879. DOI
7	Kang, K. and Choi, H.T. 2012. Growth inhibition of plant pathogenic fungi by a chitinase of Coprinellus congregates. Korean J. Microbiol. 48, 325-327. DOI
8	Kang, Y., Kim, H., and Choi, H.T. 2013. Biochemical characterization of chitinase 2 expressed during the autolytic phase of the inky cap, Coprinellus congregatus. J. Microbiol. 51, 189-193. DOI
9	Lee, K.K., MacCallum, D.M., Jacobsen, M.D., Walker, L.A., Odds, F.C., Gow, N.A.R., and Munro, C.A. 2012. Elevated cell wall chitin in Candida albicans confers echinocandin resistance in vivo. Antimicrob. Agents Chemother. 56, 208-217. DOI
10	Lopez-Matas, M.A., Aslava, A.P., and Diaz-Minguez, J.M. 2000. Mcchs1, a member of a chitin synthase gene family in Mucor circinelloides, is differentially expressed during dimorphism. Microbiology 40, 169-175.
11	Lyons, T.W., Wakefield, D.B., and Cloutier, M.M. 2011. Mold and Alternaria skin test reactivity and asthma in children in Connecticut. Ann. Allergy Asthma Immunol. 106, 301-307. DOI
12	Sharpe, R.A., Bearman, N., Thornton, C.R., Husk, K., and Osborne, N.J. 2015a. Indoor fungal diversity and asthma: a meta-analysis and systemic review of risk. J. Allergy Clin. Immunol. 135, 110-122. DOI
13	Sharpe, R.A., Thornton, C.R., Tyrrell, J., Nikolaou, V., and Osborne, N.J. 2015b. Variable risk of atopic disease due to indoor fungal exposure in NHANES 2005-2006. Clin. Exp. Allergy 45, 1478-1482. DOI
14	Yoo, Y. and Choi, H.T. 2014. Antifungal chitinase against human pathogenic yeasts from Coprinellus congregates. Korean J. Microbiol. 52, 441-443.
15	Zhao, J., Li, S., Jiang, T., Liu, Z., Zhang, W., Jian, G., and Qi, F. 2012. Chilling stress-the key predisposing factor for causing Alternaria alternata infection and leading to cotton (Gossypium hirsutum L.) leaf senescence. PLoS One 7, 1-11.