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http://dx.doi.org/10.7845/kjm.2016.6020

Characterization of heterotrophic nitrification and aerobic denitrification by Alcaligenes faecalis NS13  

Jung, Taeck-Kyung (Department of Biological Sciences, Kangwon National University)
Ra, Chang-Six (Division of Animal Resource Science, Kangwon National University)
Joh, Ki-Seong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.52, no.2, 2016 , pp. 166-174 More about this Journal
Abstract
In order to find an efficient bacterial strain that can carry out nitrification and denitrification simultaneously, we isolated many heterotrophic nitrifying bacteria from wastewater treatment plant. One of isolates NS13 showed high removal rate of ammonium and was identified as Alcaligenes faecalis by analysis of its 16S rDNA sequence, carbon source utilization and fatty acids composition. This bacterium could remove over 99% of ammonium in a heterotrophic medium containing 140 mg/L of ammonium at pH 6-9, $25-37^{\circ}C$ and 0-4% of salt concentrations within 2 days. It showed even higher ammonium removal at higher initial ammonium concentration in the medium. A. faecalis NS13 could also reduce nitrate and nitrous oxide by nitrate reductase and nitrous oxide reductase, respectively, which was confirmed by detection of nitrate reductase gene, napA, and nitrous oxide reducase gene, nosZ, by PCR. One of metabolic intermediate of denitrification, $N_2O$ was detected from headspace of bacterial culture. Based on analysis of all nitrogen compounds in the bacterial culture, 42.8% of initial nitrogen seemed to be lost as nitrogen gas, and 46.4% of nitrogen was assimilated into bacterial biomass which can be removed as sludge in treatment processes. This bacterium was speculated to perform heterotrophic nitrification and aerobic denitrification simultaneously, and may be utilized for N removal in wastewater treatment processes.
Keywords
Alcaligenes faecalis; aerobic denitrification; heterotrophic nitrification; nitrogen removal;
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1 APHA, AWWA, and WPCF. 1995. Standard methods for the examination of water and wastewater, 19th ed. pp. 483-484. American Public Health Association, Washington, DC, USA.
2 Castignetti, D. and Hollocher, T. 1981. Vigorous denitrification by a heterotrophic nitrifier of the genus Alcaligenes. Curr. Microbiol. 6, 229-231.   DOI
3 Chang, C.H. and Hao, O.J. 1996. Sequencing batch reactor system for nutrient removal: ORP and pH profiles. J. Chem. Technol. Biotechnol. 67, 27-38.   DOI
4 Chen, P., Li, J., Li, Q.X., Wang, Y., Li, S., Ren, T., and Wang, L. 2012. Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24. Bioresour. Technol. 116, 266-270.   DOI
5 Enwall, K., Philippot, L., and Hallin, S. 2005. Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization. Appl. Environ. Microbiol. 71, 8335-8343.   DOI
6 Fdz-Polanco, F., Villaverde, S., and Garcia, P. 1994. Temperature effect on nitrifying bacteria activity in biofilters: activation and free ammonia inhibition. Water Sci. Technol. 30, 121-130.
7 Ferree, M. and Shannon, R. 2001. Evaluation of a second derivative UV/visible spectroscopy technique for nitrate and total nitrogen analysis of wastewater samples. Water Res. 35, 327-332.   DOI
8 Foss, S., Heyen, U., and Harder, J. 1998. Alcaligenes defragrans sp. nov., description of four strains isolated on alkenoic monoterpenes (+)-menthene, ${\alpha}$-pinene, 2-carene, and ${\alpha}$-phellandrene and nitrate. Syst. Appl. Microbiol. 21, 237-244.   DOI
9 Frear, D. and Burrell, R. 1955. Spectrophotometric method for determining hydroxylamine reductase activity in higher plants. Anal. Chem. 27, 1664-1665.   DOI
10 Garland, J. and Mills, A. 1991. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl. Environ. Microbiol. 57, 2351-2359.
11 Klatte, T., Evans, L., Whitehead, R.N., and Cole, J. 2011. Four PCR primers necessary for the detection of periplasmic nitrate reductase genes in all groups of proteobacteria and in environmental DNA. Biochem. Soc. Trans. 39, 321-326.   DOI
12 Ludzack, F. and Noran, D. 1965. Tolerance of high salinities by conventional wastewater treatment processes. J. Water Poll. Control Fed. 37, 1404-1416.
13 Nishio, T., Yoshikura, T., Mishima, H., Inouye, Z., and Itoh, H. 1998. Conditions for nitrification and denitrification by an immobilized heterotrophic nitrifying bacterium Alcaligenes faecalis OKK17. J. Ferment. Bioeng. 86, 351-356.   DOI
14 Padhi, S., Tripathy, S., Sen, R., Mahapatra, A., Mohanty, S., and Maiti, N. 2013. Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater. Int. Biodet. Biodeg. 78, 67-73.   DOI
15 Rehfuss, M. and Urban, J. 2005. Alcaligenes faecalis subsp. phenolicus subsp. nov. a phenol-degrading, denitrifying bacterium isolated from a graywater bioprocessor. Syst. Appl. Microbiol. 28, 421-429.   DOI
16 Sarioglu, O., Suluyayla, R., and Tekinay, T. 2012. Heterotrophic ammonium removal by a novel hatchery isolate Acinetobacter calcoaceticus STB1. Int. Biodet. Biodeg. 71, 67-71.   DOI
17 Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. MIDI., Newark, Del, USA.
18 Van Loosdrecht, M. and Jetten, M. 1998. Microbiological conversions in nitrogen removal. Water Sci. Technol. 38, 1-7.
19 Schroll, G., Busse, H., Busse, H., Parrer, G., Rolleke, S., Lubitz, W., and Denner, E. 2001. Alcaligenes faecalis subsp. parafaecalis subsp. nov., a bacterium accumulating poly-${\beta}$-hydroxybutyrate from acetone-butanol bioprocess residues. Syst. Appl. Microbiol. 24, 37-43.   DOI
20 Shi, Z., Zhang, Y., Zhou, J., Chen, M., and Wang, X. 2013. Biological removal of nitrate and ammonium under aerobic atmosphere by Paracoccus versutus LYM. Bioresour. Technol. 148, 144-148.   DOI
21 Van Trappen, S., Tan, T., Samyn, E., and Vandamme, P. 2005. Alcaligenes aquatilis sp. nov., a novel bacterium from sediments of the Weser Estuary, Germany, and a salt marsh on Shem Creek in Charleston Harbor, USA. Int. J. Syst. Evol. Microbiol. 55, 2571-2575.   DOI
22 Won, S., Cho, W., Lee, J., Park, K., and Ra, C. 2014. Data build-up for the construction of Korean specific greenhouse gas emission inventory in livestock categories. Asian-Australas. J. Anim. Sci. 27, 439-446.
23 Yang, X., Wang, S., Zhang, D., and Zhou, L. 2011. Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Bacillus subtilis A1. Bioresour. Technol. 102, 854-862.   DOI
24 Yokoyama, S., Adachi, Y., Asakura, S., and Kohyama, E. 2012. Characterization of Alcaligenes faecalis strain AD15 indicating biocontrol activity against plant pathogens. J. Gen. Appl. Microbiol. 59, 89-95.
25 Yoshida, T. and Alexander, M. 1970. Nitrous oxide formation by Nitrosomonas europea and heterotrophic micro-organisms. Proc. Soil Sci. Soc. Am. 34, 880-882.   DOI
26 Zhao, B., An, Q., He, Y., and Guo, J. 2012. $N_2O$ and $N_2$ production during heterotrophic nitrification by Alcaligenes faecalis strain NR. Bioresour. Technol. 116, 379-385.   DOI
27 Zhang, Q., Liu, Y., Ai, G., Miao, L., Zheng, H., and Liu, Z. 2012. The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresour. Technol. 108, 35-44.   DOI
28 Zhang, J., Wu, P., Hao, B., and Yu, Z. 2011. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresour. Technol. 102, 9866-9869.   DOI