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http://dx.doi.org/10.7845/kjm.2016.5048

Isolation of marine algicidal bacteria from surface seawater and sediment samples associated with harmful algal blooms in Korea  

Kristyanto, Sylvia (Department of Life Science, Kyonggi University)
Kim, Jaisoo (Department of Life Science, Kyonggi University)
Publication Information
Korean Journal of Microbiology / v.52, no.1, 2016 , pp. 40-48 More about this Journal
Abstract
This study mainly focused on isolation of marine algicidal bacteria associated with phytoplankton blooms and characterization of algicidal activity against harmful algae. Harmful algal blooms (HABs) found naturally in surface waters have caused many environmental problems worldwide. In this study, forty bacterial strains that have capability of inhibiting harmful algal growth were isolated from Masan Bay, Jinhae Bay, Dol Island, Jangmok Bay, and the Tongyeong Sea, Republic of Korea. The bacteria were screened furthermore for the characteristics on algicidal activities against Cochlodinium polykrikoides, Chattonella marina, Skeletonema costatum, Heterosigma akashiwo, Heterocapsa triquetra, Prorocentrum minimum, and Scrippsiella trochoidea. As a result, the algicidal bacteria that were screened from double over layer agar and microscopic counts tests belonged to genera Pseudomonas, Vibrio, Bacillus, Pseudoalteromonas, Ruegeria, Joostella, Marinomonas, Stakelama, Porphyrobacter, and Albirhodobacter. One of the most important HAB species is Co. polykrikoides and the strongest algicidal activity against the dinoflagellate was 94.00% after 6 h treatment with 10% bacterial culture filtrate. In this study, Marinomonas sp. M Jin 1-8, Stakelama sp. ZB Yeonmyeong 1-11 & 1-13, Porphyrobacter sp. M Yeonmyeong 2-22, and Albirhodobacter sp. 6-R Jin 6-1 were found to be as new genera of bacteria having anti-algal activity. These results suggest that these bacteria might play an important role in controlling phytoplankton blooms.
Keywords
Albirhodobacter; Cochlodinium polykrikoides; Marinomonas; Porphyrobacter; Stakelama; algicidal bacteria; harmful algal blooms;
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1 Amaro, A.M., Fuentes, M.S., Ogalde, S.R., Venegas, J.A., and Suarez-Isla, B.A. 2005. Identification and characterization of potentially algal-lytic marine bacteria strongly associated with the toxic dinoflagellate Alexandrium catenella. J. Eukaryot. Microbiol. 52, 191-200.   DOI
2 Anderson, D.M. 2009. Approaches to monitoring, control and management of harmful algal blooms (HABs). Ocean Coast. Manage. 52, 342-347.   DOI
3 Anderson, D.M., Glibert, P.M., and Burkholder, J.M. 2002. Harmful algal blooms and eutrophication: nutrient sources, composition, and consequences. Estuaries 25, 704-726.   DOI
4 Byun, H.G., Jeong, S.Y., Park, Y.T., Lee, W.J., and Kim, S.K. 2002. Algicidal activity of substance purified from marine bacteria metabolites against Cochlodinium polykrikoides. J. Fish. Sci. Technol. 5, 150-155.
5 Grossart, H.P., Levold, F., Allgaier, M., Simon, M., and Brinkhoff, T. 2005. Marine diatom species harbour distinct bacterial communities. Environ. Microbiol. 7, 860-873.   DOI
6 Guillard, R.L. and Ryther, J.H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana (Hustedt), and Detonula confervacea (Cleve) Gran. Can. J. Microbiol. 8, 229-239.   DOI
7 Hallegraeff, G.M. 1993. A review of harmful algal blooms and their apparent global increase. Phycologia 32, 79-99.   DOI
8 Jung, S.W., Kim, B.H., Katano, T., Kong, D.S., and Han, M.S. 2008. Pseudomonas fluorescens HYK0210-SK09 offers species-specific biological control of winter algal blooms caused by freshwater diatom Stephanodiscus hantzschii. J. Appl. Microbiol. 105, 186-195.   DOI
9 Kang, Y.K., Cho, S.Y., and Kang, Y.H. 2008. Isolation, identification and characterization of algicidal bacteria against Stephanodiscus hantzschii and Peridinium bipes for the control of freshwater winter algal blooms. J. Appl. Phycol. 20, 375-386.   DOI
10 Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716-721.   DOI
11 Kim, M.J., Jeong, S.Y., and Lee, S.J. 2008. Isolation, identification, and algicidal activity of marine bacteria against Cochlodinium polykrikoides. J. Appl. Phycol. 20, 1069-1078.   DOI
12 Kim, J.D., Kim, B., and Lee, C.G. 2007. Alga-lytic activity of Pseudomonas fluorescens against the red tide causing marine alga Heterosigma akashiwo (Raphidophyceae). Biol. Control 41, 296-303.   DOI
13 Kim, J.D., Kim, J.Y., Park, J.K., and Lee, C.G. 2009a. Selective control of the Prorocentrum minimum harmful algal blooms by a novel algal-lytic bacterium Pseudoalteromonas haloplanktis AFMB-008041. Mar. Biotechnol. 11, 463-472.   DOI
14 Lee, S.O., Kato, J., Takiguchi, N., Kuroda, A., Ikeda, T., Mitsutani, A., and Ohtake, H. 2000. Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28. Appl. Environ. Microbiol. 66, 4334-4339.   DOI
15 Kim, Y.S., Lee, D.S., Jeong, S.Y., Lee, W.J., and Lee, M.S. 2009b. Isolation and characterization of a marine algicidal bacterium against the harmful Raphidophyceae Chattonella marina. J. Microbiol. 47, 9-18.   DOI
16 Kim, C.H., Park, T.G., and Lee, C.K. 2010. Harmful dinoflagellates and mitigation strategies in Korea. Philipp. J. Sci. 139, 139-147.
17 Kim, M.C., Yoshinaga, I., Imai, I., Nagasaki, K., Itakura, S., and Ishida, Y. 1998. A close relationship between algicidal bacteria and termination of Heterosigma akashiwo (Raphidophyceae) blooms in Hiroshima Bay, Japan. Mar. Ecol. Prog. Ser. 170, 25-32.   DOI
18 Lee, C.K., Park, T.G., Park, Y.T., and Lim, W.A. 2013. Monitoring and trends in harmful algal blooms and red tides in Korean coastal waters, with emphasis on Cochlodinium polykrikoides. Harmful Algae 30S, S3-S14.
19 Lei, X., Li, Y., Chen, Z., Zheng, W., Lai, Q., Zhang, H., Guan, C., Cai, G., Yang, X., Tian, Y., et al. 2014. Altererythrobacter xiamenensis sp. nov., an algicidal bacterium isolated from red tide seawater. Int. J. Syst. Evol. Microbiol. 64, 631-637.   DOI
20 Mayali, X. and Azam, F. 2004. Algicidal bacteria in the sea and their impact on algal blooms. J. Eukaryot. Microbiol. 51, 139-144.   DOI
21 Nagasaki, K., Tomaru, Y., Katanozaka, N., Shirai, Y., Nishida, K., Itakura, S., and Yamaguchi, M. 2004. Isolation and characterization of a novel single-stranded RNA virus infecting the bloom-forming diatom Rhizosolenia setigera. Appl. Environ. Microbiol. 70, 704-711.   DOI
22 Roth, P.B., Twiner, M.J., Mikulski, C.M., Barnhorst, A.B., and Doucette, G.J. 2008. Comparative analysis of two algicidal bacteria active against the red tide dinoflagellate Karenia brevis. Harmful Algae 7, 682-691.   DOI
23 Oh, J.I., Kim, M.J., Lee, J.Y., Ko, I.J., Kim, W., and Kim, S.W. 2011. Isolation and characterization of algicidal bacteria from Cochlodinium polykrikoides culture. Biotechnol. Bioproc. Eng. 16, 1124-1133.   DOI
24 Park, J., Jeong, H.J., Yoo, Y.D., and Yoon, E.Y. 2013. Mixotrophic dinoflagellate red tides in Korean waters: Distribution and ecophysiology. Harmful Algae 30S, S28-S40.
25 Park, S.H., Kwon, K.K., Lee, D.S., and Lee, H.K. 2002. Morphological diversity of marine microorganisms on different isolation media. J. Microbiol. 40, 161-165.
26 Sengco, M.R. and Anderson, D.M. 2004. Controlling harmful algal blooms through clay flocculation. J. Eukaryot. Microbiol. 51, 169-172.   DOI
27 Seong, K.A. and Jeong, H.J. 2013. Interactions between marine bacteria and red tide organisms in Korean waters. Algae 28, 297-305.   DOI
28 Shi, R., Huang, H., Qi, Z., Hu, W., Tian, Z., and Dai, M. 2013. Algicidal activity against Skeletonema costatum by marine bacteria isolated from a high frequency harmful algal blooms area in southern Chinese coast. World J. Microbiol. Biotechnol. 29, 153-162.   DOI
29 Yang, X., Li, X., Zhou, Y., Zheng, W., Yu, C., and Zheng, T. 2014. Novel insights into the algicidal bacterium DH77-1 killing the toxic dinoflagellate Alexandrium tamarense. Sci. Total Environ. 482-483, 116-124.   DOI
30 Yang, L., Maeda, H., Yoshikawa, T., and Zhou, G.Q. 2012. Algicidal effect of bacterial isolates of Pedobacter sp. against cyanobacterium Microcystis aeruginosa. Water Sci. Eng. 5, 375-382.
31 Zingone, A. and Enevoldsen, H.O. 2000. The diversity of harmful algal blooms: a challenge for science and management. Ocean Coast. Manage. 43, 725-748.   DOI
32 Yang, F., Wei, H.Y., Li, X.Q., Li, Y.H., Li, X.B., Yin, L.H., and Pu, Y.P. 2013. Isolation and characterization of an algicidal bacterium indigenous to lake Taihu with a red pigment able to lyse Microcystis aeruginosa. Biomed. Environ. Sci. 26, 148-154.
33 Zheng, X., Zhang, B., Zhang, J., Huang, L., Lin, J., Li, X., Zhou, Y., Wang, H., Yang, X., Su, J., et al. 2013. A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa. Appl. Microbiol. Biotechnol. 97, 9207-9215.   DOI