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http://dx.doi.org/10.7845/kjm.2015.5007

Isolation and characterization of Bacillus amyloliquefaciens TK3 inhibiting causative bacteria of atrophic rhinitis and fowl typhoid  

Jung, Taeck-Kyung (Department of Biological Sciences, Kangwon National University)
Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.51, no.2, 2015 , pp. 177-180 More about this Journal
Abstract
For prevention of atrophic rhinitis of swine by Bordetella bronchiseptica and fowl typhoid by Salmonella gallinarum, bacterial strains showing antimicrobial activity against those pathogenic bacteria were isolated from various samples collected at animal farms. Among 372 bacterial isolates strain TK3 showed the highest antibacterial activity against both pathogens, and was identified as Bacillus amyloliquefaciens by 16S rRNA gene sequence analysis. B. amyloliquefaciens TK3 could inhibit growth of both pathogens by secretion of antibacterial compounds such as siderophore, rhamnolipid and antimicrobial peptide. Production radius of siderophore on Chrome azurol S agar plate by strain TK3 was 0.53 cm after 14 days of incubation, and concentration of siderophore in King's B medium was 1.06 mmol/ml. It also secreted 82.4 mg/L of rhamnolipid, and antimicrobial peptide that completely inhibited growth of both pathogens at concentration of $30{\mu}l/ml$ in LB medium.
Keywords
Bacillus amyloliquefaciens; Bordetella bronchiseptica; Salmonella gallinarum; antibacterial activity;
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