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http://dx.doi.org/10.7845/kjm.2014.4068

Isolation and Identification of Pseudomonas aeruginosa in Natural Environments by International Organization for Standardization ISO/NP 16266  

Lee, Siwon (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Kim, Ji Hye (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Lee, Bo-Ram (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Joo, Youn-Lee (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Choe, Byeol (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Park, Su Jeong (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Chung, Hyen-Mi (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Jheong, Weon Hwa (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Publication Information
Korean Journal of Microbiology / v.50, no.4, 2014 , pp. 384-386 More about this Journal
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that inhabits various natural and artificial environments, such as pathogenesis, water, soil and air. They can cause serious problems, such as pathogenic infection. In this study, 220 colonies were isolated from water and soil environment that assumed to be P. aeruginosa using a membrane filter method based on International Organization for Standardization (ISO/NP 16266). Identification of the isolates was determined by physiobiochemical characteristics using newly modified ISO method which includes the resistance to 1,10 phenanthroline test. Only one of 220 presumed P. aeruginosa strains isolated from effluence water using a drain swab was determined as P. aeruginosa-positive by the ISO/NP 16266 method. Subsequently, the resistance to 1,10 phenanthroline test, which was newly proposed by ISO in 2014 and applied in this study, was considered as more precise and improvable method for identification of P. aeruginosa.
Keywords
Pseudomonas aeruginosa; International Organization for Standardization; ISO/NP 16266;
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