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http://dx.doi.org/10.7845/kjm.2014.4039

Phenotypic Characteristics of Pseudomonas syringae pv. actinidiae Strains from Different Geographic Origins  

Choi, Eun Jin (Department of Biology, Sunchon National University)
Lee, Young Sun (Department of Biology, Sunchon National University)
Kim, Gyoung Hee (Department of Plant Medicine, Sunchon National University)
Koh, Young Jin (Department of Plant Medicine, Sunchon National University)
Jung, Jae Sung (Department of Biology, Sunchon National University)
Publication Information
Korean Journal of Microbiology / v.50, no.3, 2014 , pp. 245-248 More about this Journal
Abstract
Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker in kiwifruit (genus Actinidia). Multilocus sequence analysis of seven housekeeping and 11 type III effector genes differentiated the virulent P. syringae pv. actinidiae isolates worldwide into three groups designated as Psa1-Psa3. In this work, a total of 12 P. syringae pv. Actinidiae strains, including three Psa1, three Psa2, three Psa3 strains isolated from Korea and three Psa3 strains from Italy, were compared based on their phenotypic properties. Strains with different geographic origins had unique growth patterns as demonstrated by growth rate at several temperatures; all tested strains exhibited maximum growth at temperatures below $22^{\circ}C$, while the growth of Psa3 strains was completely inhibited above $30^{\circ}C$. Psa3 strains isolated from Korea had longer lag phases than the Psa3 strains from Italy. The Psa2 strains were different from Psa1 and Psa3 strains in the API 20NE test, in which the Psa2 strains could not utilize potassium gluconate, capric acid and trisodium citrate. Psa3 strains isolated from Korea could hydrolyze esculin. The API ZYM test showed that ${\beta}$-glucosidase activity was detected only from Psa3 strains. The strains belonging to the three Psa groups differed with regard to their susceptibility to ampicillin, novobiocin, and oleandomycin.
Keywords
Pseudomonas syringae pv. actinidiae; bacterial canker; kiwifruit; phenotype;
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1 Koh, Y.J., Cha, B.J., Chung, H.J., and Lee, D.H. 1994. Outbreak and spread of bacterial canker in kiwifruit. Kor. J. Plant Pathol. 10, 68-72.   과학기술학회마을
2 Koh, Y.J., Kim, G.H., Koh, H.S., Lee, Y.S., Kim, S.C., and Jung, J.S. 2012. Occurrence of a new type of Pseudomonas syringae pv. actinidiae strain of bacterial canker on kiwifruit in Korea. Plant Pathol. J. 28, 423-427.   DOI
3 Mazzaglia, A., Studholme, D.J., Taratufolo, M.C., Cai, R., Almeida, N.F., Goodman, T., Guttman, D.S., Vinatzer, B.A., and Balestra, G.M. 2012. Pseudomonas syringae pv. actinidiae (PSA) isolates from recent bacterial canker of kiwifruit outbreaks belong to the same genetic lineage. PLoS ONE 7, e36518.   DOI
4 Scotichini, M. 1994. Occurrence of Pseudomonas syringae pv. actinidiae on kiwifruit in Italy. Plant Pathol. 43, 1035-1038.   DOI   ScienceOn
5 Takikawa, Y., Serizawa, S., Ichikawa, T., Tsuyumu, S., and Goto, M. 1989. Pseudomonas syringae pv. actinidiae sp. nov., the causal bacterium of canker in kiwifruit in Japan. Ann. Phytopathol. Soc. Japan 55, 437-444.   DOI
6 Vanneste, J.L. 2012. P. syringae pv. actinidiae (Psa): a threat to the New Zealand and global kiwifruit industry. N. Z. J. Crop Horticul. Sci. 40, 265-267.   DOI
7 Vanneste, J.L., Poliakoff, F., Audusseau, C., Cornish, D.A., Pailard, S., Rivoal, C., and Yu, J. 2011. First report of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit in France. Plant Dis. 95, 1311.
8 Bastas, K. and Karakaya, A. 2012. First report of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae in Turkey. Plant Dis. 96, 452.
9 Butler, M.I., Jung, J.S., Kim, G.H., Lamont, I., Stockwell, P., Koh, Y.J., and Poulter, R.T.M. 2013a. Genomic features of Pseudomonas syringae pv. actinidiae recently isolated in Korea. p. 84. Abstr. 1st International Symposium on Bacterial Canker of Kiwifruit (Psa).
10 Butler, M.I., Stockwell, P.A., Black, M.A., Day, R.C., Lamont, I.L., and Poulter, R.T.M. 2013b. Pseudomonas syringae pv. actinidiae from recent outbreaks of kiwifruit bacterial canker belong to different clones that originated in China. PLoS ONE 8, e57464.   DOI
11 Everett, K.R., Taylor, R.K., Romberg, M.K., Rees-George, J., Fullerton, R.A., Vanneste, J.L., and Manning, M.A. 2011. First report of Pseudomonas syringae pv. actinidiae causing kiwifruit bacterial canker in New Zealand. Aust. Plant Dis. Notes 6, 67-71.   DOI
12 Koh, H.S., Kim, G.H., Lee, Y.S., Koh, Y.J., and Jung, J.S. 2014. Molecular characteristics of Pseudomonas syringae pv. actinidiae strains isolated in Korea and a multiplex PCR assay for haplotype differentiation. Plant Pathol. J. 30, 96-101.   DOI
13 Ferrante, P. and Scortichini, M. 2009. Identification of Pseudomonas syringae pv. actinidiae as causal agent of bacterial canker of yellow kiwifruit (Actinidia chinensis Planchon) in central Italy. J. Phytopathol. 157, 768-770.   DOI
14 Ferrante, P. and Scortichini, M. 2010. Molecular and phenotypic features of Pseudomonas syringae pv. actinidiae isolated during recent epidemics of bacterial canker on kiwifruit (Actinidia chinensis) in central Italy. Plant Pathol. 59, 954-962.   DOI
15 Han, H.S., Koh, Y.J., Hur, J.S., and Jung, J.S. 2003. Identification and characterization of coronatine-producing Pseudomonas syringae pv. actinidiae. J. Microbiol. Biotechnol. 13, 110-118.
16 Balestra, G.M., Taratufolo, M.C., Vinatzer, B.A., and Mazzaglia, A. 2013. A multiplex PCR assay for detection of Pseudomonas syringae pv. actinidiae and differentiation of populations with different geographic origin. Plant Dis. 97, 472-478.   DOI
17 Anonymous. 2011. Bacterial canker, kiwifruit-Chile: First report (O'Higgins, Maule). ProMed mail: International Society for Infectious Disease.
18 Balestra, G.M., Renzi, M., and Mazzaglia, A. 2010. First report of bacterial canker of Actinidia deliciosa caused by Pseudomonas syringae pv. actinidiae in Portugal. New Dis. Rep. 22, 10.   DOI
19 Balestra, G.M., Renzi, M., and Mazzaglia, A. 2011. First report of Pseudomonas syringae pv. actinidiae on kiwifruit plants in Spain. New Dis. Rep. 24, 10.   DOI
20 Chapman, J.R., Taylor, R.K., Weir, B.S., Romberg, M.K., Vanneste, J.L., Luck, J., and Alexander, B.J.R. 2012. Phylogenetic relationships among global populations of Pseudomonas syringae pv. actinidiae. Phytopathology 102, 1034-1044.   DOI