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http://dx.doi.org/10.7845/kjm.2014.4020

The Interaction between HCV-Infected huh7.5 Cells and HCV-Specific T Cells  

Kang, Hyojeung (Department of Pharmacy, College of Pharmacy, Kyungpook National University)
Cho, Hyosun (Department of Pharmacy, College of Pharmacy, Duksung Women's University)
Publication Information
Korean Journal of Microbiology / v.50, no.2, 2014 , pp. 169-172 More about this Journal
Abstract
Recently, Hepatitis C virus (HCV) replication system has been established using human hepatoma cells (huh cell) and a variety of HCV clones. In this study, we established an infectious HCV replication system using huh7.5 cells and J6/JFH1 clone (genotype 2a). In addition, we investigated the antigen presentation capability of HCV-infected huh7.5 cells to HCV-specific T cells. Interestingly, HCV-infected huh7.5 cells were not capable of activating HCV-specific T cells. However, huh7.5 cells stimulated by exogenous HCV peptide were able to activate HCV-specific T cells, which was shown to produce TNF-${\alpha}$ and IFN-${\gamma}$. We further examined if HCV infection has an inhibitory effect on the expression of MHC class I molecule of huh7.5 cells. We found that HCV infection did not change the expression level of MHC class I molecule on huh7.5 cells.
Keywords
HCV-specific T cells; hepatoma cell 7.5 (huh7.5); IFN-${\gamma}$; J6/JFH1 clone; TNF-${\alpha}$;
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