Browse > Article
http://dx.doi.org/10.7845/kjm.2014.4032

bla Genotype and Molecular Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Chungcheong Regional Hospitals  

Yook, Keun Dol (Department of Clinical Laboratory Science, Daejeon Health Science College)
Yang, Byoung Seon (Department of Medical Laboratory Science, Jinju Health College)
Park, Jin Sook (Department of Biological Sciences and Biotechnology, Hannam University)
Publication Information
Korean Journal of Microbiology / v.50, no.2, 2014 , pp. 114-118 More about this Journal
Abstract
A total of 122 ESBL-producing intestinal bacteria were collected from regional hospitals in the Chungcheong area. Combination disk test (CDT) was performed for antimaicrobial susceptability using cefotaxime and cefotaxime/clavulanate according to Clinical Laboratory Standard Institute (CLSI). Mutiplex PCR using specific primers was performed for a detection of ESBL-genotypes and enterobacterial repetitive intergenic consensus (ERIC)-PCR was carried out for the tracking of molecular epidemiology. In the confirmation test using CDT, 73 out of 76 (96.1%) ESBL-producing Escherichia coli and 43 out of 46 (93.4%) ESBL-producing Klebsiella pnemoniae were positive. In the multiplex PCR, 60.5% of E. coil were positive for CTX-M-2 type gene and 56.5% of K. pneumoniae were positive for VEB -1 type gene. In the ERIC-PCR, E. coil isolates formed 5 clusters and K. pneumoniae isolates were grouped into 4 clusters depending on region. Genotypes of clinical isolates are useful for detection and differentiation of ESBL producing intestinal bacteria. The ERIC-PCR method is thought to be helpful for establishing a regional surveillance system for infection due to its formation of different clusters depending on region.
Keywords
Escherichia coli; Klebsiella pnemoniae; CDT; ERIC-PCR; ESBL; multiplex PCR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Korea Centers for Disease Prevention and Control. 2013. Monitoring of antimicrobial resistance on non-tertiary hospitals in Korea, 2007-2011, Annual report. 12-21.
2 Ruppe, E., Bidet, P., Verdet, C., Arlet, G., and Bingen, E. 2006. First detection of the Ambler class C1 AmpC $\beta$-lactamases in Citrobacter frunddii by a new, simple double-disk synergy test. J. Clin. Microbiol. 44, 4204-4207.   DOI   ScienceOn
3 Shin, K.S. and Son, B.R. 2002. Comparison of Vitek ESBL test and other methods for detecting extended-spectrum $\beta$-lactamase producing Escherichia coli and Klebsiella species. Korean J. Clin. Pathol. 22, 21-26.
4 Silva, J., Gatica, R., Aguilar, C., Becerra, Z., Garza-Ramos, U., Velazquez, M., Miranda, G., Leanos, B., Solorzano, F., and Echaniz, G. 2001. Outbreak of infection with extended-spectrum beta-lactamase producing Klebsiella pneumoniae in a Mexican hospital. J. Clin. Microbiol. 39, 3193-3196.   DOI   ScienceOn
5 Steward, C.D., Rasheed, K., Hubert, S.K., Biddle, J.W., Raney, P.M., Anderson, G.J., Williams, P.P., Brittain, K.L., Oliver, A., McGowan, J.E. Jr., Tenover, F.C., and et al. 2001. Characterization of clinical isolates of Klebsiella pneumoniae from 19 laboratories using the National Committee for Clinical Laboratory Standards extended-spectrum $\beta$ -lactamase detection methods. J. Clin. Microbiol. 39, 2864-2872.   DOI   ScienceOn
6 Lee, B.Y., Jeong, S.H., Jeong, T.S., Nam, H.J., Ji, J.H., and Hong, Y.R. 2001. Detection of extended-spectrum $\beta$-lactamase producing Escherichia coli and Klebsiella spp. with the Vitek GNS 121 card. Korean J. Clin. Pathol. 21, 350-354.
7 Lee, J.H., Bae, I.K., Kwon, S.B., Jeong, S.H., Woo, G.J., Lee, J.W., Lee, W.G., Kang, J.O., Ahn, J.Y., Hong, S.G., and et al. 2004. Prevalence of CTX-M-type extended-spectrum $\beta$-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates in Korea, 2003. Korean J. Clin. Microbiol. 7, 111-118.
8 Lee, K.W., Jo, S.R., Lee, C.S., Jung, Y.S., and Gwon, O.H. 1996. Extended spectrum $\beta$-lactamase producing Escherichia coli and Klebsiella pneumoniae. Infection 26, 341-348.
9 Monica, C., Sonia, M.T., Alejandro, P., Angeles, B.N., David, D., Francisca, V., Rosa, M., and German, V. 2004. Risk factors for colonization and infection in a hospital outbreak caused by a strain of Klebsiella pneumoniae with reduced susceptibility to expanded-spectrum cephalosporins. J. Clin. Microbiol. 42, 42-49.
10 Pai, H.J. 1998. The characteristics of extended-spectrum $\beta$-lactamases in Korean isolates of Enterobacteriaceae. Yonsei Med. J. 39, 514-519.   DOI   ScienceOn
11 Edelstein, M., Pimkin, M., Palagin, I., Edelstein, I., and Stratchounski, L. 2003. Prevalence and molecular epidemiology of CTX-M extended-spectrum $\beta$-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Russian hospitals. Antimicrob. Agents Chemother. 47, 3724-3732.   DOI   ScienceOn
12 Park, J.H., Lee, S.H., Jeong, S.H., Kim, B.N., Kim, K.B., Yoon, J.D., and Jeon, B.C. 2003. Characterization and prevalence of Escherichia coli and Klebsiella pneumoniae isolates producing an extendedspectrum $\beta$-lactamase from Korean hospitals. Korean J. Lab. Med. 23, 18-24.
13 Poirel, L., Naas, T., Guibert, M., Chaibi, E.B., Labia, R., and Nordmann, P. 1999. Molecular and biochemical characterization of VEB, a novel class an extended-spectrum $\beta$-lactamase encoded by an Escherichia coli integron gene. Antimicrob. Agents Chemother. 43, 573-581.
14 Clinical and Laboratory Standards Institute. 2011. Performance standards for antimicrobial susceptibility testing, Document M100-S21. CLSI. Wayne, Pensylvania, USA.
15 Hong, S.G., Kang, M.S., Choi, J.R., Lee, K.W., Chong, Y.S., and Kwon, O.H. 2001. Molecular characteristics of extended-spectrum $\beta$ -lactamases in clinical isolates of Enterobacteriaceae. Korean J. Clin. Pathol. 21, 495-504.
16 Jacoby, G.A. and Medeiros, A.A. 1991. More extended-spectrum $\beta$ -latamases. Antimicrob. Agents Chemother. 35, 1697-1704.   DOI   ScienceOn
17 Jarlier, V., Nicolas, M.H., Fournier, G., and Philippon, A. 1988. Extended broadspectrum $\beta$-lactamases conferring transferable resistance to newer $\beta$-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev. Infect. Dis. 10, 867-878.   DOI
18 Bradford, P.A. 2001. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14, 933-951.   DOI   ScienceOn