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http://dx.doi.org/10.7845/kjm.2013.3067

Phylogenetic Analysis of Bacterial Populations in a Tomato Rhizosphere Soil Treated with Chicken Feather Protein Hydrolysate  

Kim, Se-Jong (Department of Microbial & Nano Materials, Mokwon University)
Han, Song-Ih (Department of Microbial & Nano Materials, Mokwon University)
Whang, Kyung-Sook (Department of Microbial & Nano Materials, Mokwon University)
Publication Information
Korean Journal of Microbiology / v.49, no.4, 2013 , pp. 328-335 More about this Journal
Abstract
As a result of conducting a cultural experiment of tomato using chicken feather protein hydrolysate (CPH) which was mass produced by keratin protein degrading bacterium Chryseobacterium sp. FBF-7 (KACC 91463P), we found that the stem and the root of tomato showed significant improvement in growth. For the purpose of phylogenic interpretation, a comparison was drawn between the effect of CPH, a treated CPH and untreated, on the changes of bacterial populations by 454 pyrosequencing based on 16S rRNA gene sequences. Tomato rhizosphere soil untreated with CPH (NCPH) showed 6.54 Shannon index from 3,281 sequence reads, and the rhizosphere soil treated with CPH (TCPH) showed 6.33 Shannon index from 2,167 sequence reads, displaying that it does not affect the diversity. Bacterial populations were composed of 19 phyla in the rhizosphere soil, and the phylum Proteobacteria occupied 40% of total bacterial populations. Bradyrhizobium, Agromonas, Nitrobacter, and Afipia (BANA group) which belong to Bradyrhizobiaceae were abundant and commonly detected in both the treated and untreated soils, suggesting the dominance of bacterial group in rhizosphere soil. The results obtained showed that CPH treatment does not affect the indigenous bacterial populations present in the rhizosphere soil.
Keywords
Bradyrhizobiaceae; chicken feather protein hydrolysate; pyrosequencing; tomato rhizosphere soil;
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1 Bertsch, A. and Coello, N. 2005. A biotechnological process for treatment and recycling poultry feathers as a feed ingredient. Bioresour. Technol. 96, 1703-1708.   DOI   ScienceOn
2 Bottomley, P.J. 1992. Ecology of Bradyrhizobium and Rhizobium. In Stacey, G., Burris, R.H., and Evans, H.J. (eds.), Biological nitrogen fixation, pp. 293-348. Chapman & Hall, New York, USA.
3 Brockwell, J. 2004. Abundant, cheap nitrogen for Australian farmers:a history of Australian nodulation and nitrogen fixation conferences. Soil Biol. Biochem. 36, 1195-1204.   DOI   ScienceOn
4 Brockwell, J., Holliday, R.A., and Pilka, A. 1988. Evaluation of the symbiotic nitrogen-fixing potential of soils by direct microbiological means. Plant Soil 108, 163-170.   DOI
5 Cole, J.R., Chai, B., Farris, R.J., Wang, Q., Kulam, S.A., McGarrell, D.M., Garrity, G.M., and Tiedje, J.M. 2005. The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res. 33, D294-D296.
6 Gupta, R. and Ramnani, P. 2006. Microbial keratinase and their prospective application: An overview. Appl. Microbiol. Biotechnol. 70, 21-33.   DOI   ScienceOn
7 Hattori, R. and Hattori, T. 1980. Sensitivity to salts and organic compounds of soil bacteria isolated on diluted media. J. Gen. Appl. Microbiol. 26, 1-14.   DOI
8 Hong, S.J., Namkung, H., Kim, W.Y., and Paik, I.K. 2002. Effects of supplemental feather digests on the growth of broiler chicks and taurine content in the broiler meat. Kor. J. Poult. Sci. 29, 141-147.   과학기술학회마을
9 Hood, C.M. and Healy, M.G. 1994. Bioconversion of waste keratins:wool and feathers. Resour. Conserv. Recycl. 11, 179-188.   DOI   ScienceOn
10 Kasahara, Y. and Hattori, T. 1991. Analysis of bacterial populations in a grassland soil according to rates of development on solid media. FEMS Microbiol. Ecol. 86, 95-102.   DOI
11 Kim, S.J., Cho, C.H., and Whang, K.S. 2010. Isolation and characterization of keratinolytic protein chicken feather-degrading bacteria. Kor. J. Microbiol. 46, 86-92.   과학기술학회마을
12 Kim, S.J., Cho, C.H., and Whang, K.S. 2011. Characterization of protease produced by Elizabethkingia meningoseptica CS2-1 and optimization of cultural conditions for amino acid production. J. Appl. Biol. Chem. 46, 86-92.
13 Kim, J.H. and Ko, Y.D. 2005. Effect of dietary protease (bromelain) treated feather meal on the performance and nutrient utilization in broilers. J. Anim. Sci. Technol. 47, 221-232.   과학기술학회마을   DOI   ScienceOn
14 Minamisawa, K., Seki, T., Onodera, S., Kubota, M., and Asami, T. 1992. Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics. Appl. Environ. Microbiol. 58, 2832-2839.
15 Minamisawa, K. and Mitsui, H. 2000. Genetic ecology of soybean bradyrhizobia. In Soil Biology (Bollag J. and Stotzky G., eds.), Vol 10, pp. 349-377. Marcel Dekker, New York, USA.
16 Onifade, A.A., Al-Sane, N.A., Al-Musallam, A.A., and Al-Zarban, S. 1998. A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour. Technol. 66, 1-11.   DOI   ScienceOn
17 Minamisawa, K., Nakatsuka, Y., and Isawa, T. 1999. Diversity and field site variation of indigenous populations of soybean bradyrhizobia in Japan by fingerprints with repeated sequences $RS{\alpha}$ and $RS{\beta}$. FEMS Microbiol. Ecol. 29, 171-178.
18 Ohta, H. and Hattori, T. 1980. Bacteria sensitive to nutrient broth medium in terrestrial environments. Soil Sci. Plant Nutr. 26, 99-107.   DOI
19 Ohta, H. and Hattori, T. 1983. Agromonas oligotrophica gen. nov. sp. nov. a nitrogen-fixing oligotrophic bacterium. Antonie van Leewenhoek 49, 429-446.
20 SAS Institute. 1988. SAS/STAT user's guide, release 6.03. SAS Institute, Cary, North Carolina, USA.
21 Schloss, P.D., Westcott, S.L., Ryabin, T., Hall, J.R., Hartmann, M., Hollister, E.B., Lesniewski, R.A., Oakley, B.B., Parks, D.H., Robinson, C.J., and et al. 2009. Introducing mothur:Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol. 75, 7537-7541.   DOI   ScienceOn
22 Takada-Hoshino, Y. and Matsumoto, N. 2004. An improved DNA extraction method using skim milk from soils that strongly adsorb DNA. Microbes Environ. 19, 13-19.   DOI   ScienceOn
23 Vignardet, C., Guillaume, Y.C., Friedrich, J., and Millet, J. 1999. A first order experimental design to assess soluble proteins released by a new keratinase from Doratomyce smicrosporus on human substrate. Int. J. Pharm. 191, 95-102.   DOI   ScienceOn
24 Whang, K.S. and Hattori, T. 1988. Oligotrophic bacteria from rendzina forest soil. Antonie van Leeuwenhoek 54, 19-36.   DOI   ScienceOn
25 Woo, E.O., Kim, M.J., Ryu, E.Y., Park, G.T., Lee, C.Y., Son, H.J., and Lee, S.J. 2007a. Isolation and application of feather-degrading bacteria for development of environment-friendly biofertilizer. J. Environ. Sci. 16, 1103-1109.   과학기술학회마을   DOI   ScienceOn
26 Yamamura, S., Morita, Y., Hasan, Q., Rao, S.R., Murakami, Y., Yokoyama, K., and Tamiya, E. 2002. Characterization of a new keratin-degrading bacterium isolated from deer fur. J. Biosci. Bioeng. 93, 595-600.   DOI   ScienceOn
27 Woo, E.O., Kim, M.J., Son, H.S., Ryu, E.Y., Jeong, S.Y., Son, H.J., Lee, S.J., and Park, G.T. 2007b. Production of protease by Bacillus pumilus RS7 and feather hydrolysate as a source of amino acids. J. Environ. Sci. 16, 1203-1208.   과학기술학회마을   DOI   ScienceOn