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http://dx.doi.org/10.7845/kjm.2013.3063

Multiplex Real-Time PCR for Simultaneous Detection of 6 Periodontopathic Bacteria  

Cho, Hong-Bum (Department of Chemical and Biological Engineering, Seokyeong University)
Publication Information
Korean Journal of Microbiology / v.49, no.3, 2013 , pp. 292-296 More about this Journal
Abstract
This study utilized an analysis method for detecting six microorganisms, such as Actinobacillus actinomycetemcomitans, Campylobacter rectus, Porphyromonas gingivalis, Tannerella forsythus, Treponema denticola, and Prevotella intermedia, triggering periodontal disease, using multiplex real-time polymerase chain reaction (PCR). The analysis including internal control was made by dividing the six species into two groups using four fluorescence dyes, and it was verified that there was no interference or cross-reaction between the target species and different kinds of oral microbial species. Qualitative and quantitative analyses were conducted on each microorganism in various samples, such as saliva and the plaque, using the multiplex real-time PCR and comparative analysis between periodontitis patients and healthy people, revealing obvious differences between them.
Keywords
multiplex real-time PCR; periodontopathic bacteria; plaque; saliva;
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