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http://dx.doi.org/10.7845/kjm.2013.3037

Construction of Amylolytic Industrial Strains of Saccharomyces cerevisiae for Improved Ethanol Production from Raw Starch  

Im, Young-Kum (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Park, Jin-Yeong (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Lee, Ja-Yeon (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Choi, Seung-Hyun (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Chin, Jong-Eon (Department of Cosmetology, Dongkang College)
Ko, Hyun-Mi (Department of Microbiology, College of Medicine, Seonam University)
Kim, Il-Chul (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Bai, Suk (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Publication Information
Korean Journal of Microbiology / v.49, no.2, 2013 , pp. 200-204 More about this Journal
Abstract
To contruct amylolytic industrial strains of Saccharomyces cerevisiae which produce ethanol efficiently from raw starch, the Bacillus amyloliquefaciens ${\alpha}$-amylase genes (Amy) or Aspergillus awamori glucoamylase genes (GA1) was separately introduced into the ribosomal DNA loci in the chromosomes of the raw starch fermenting-parental strain (ATCC 9763/$YIp{\delta}AGSA{\delta}$), using double 18S rDNA-integration system. Ethanol production after 3 days of fermentation by the strain that produced ethanol most efficiently from raw starch (ATCC 9763/$YIp{\delta}AGSA{\delta}$/YIpAG2rD) among the transformant strains was 1.5-times higher than that by the parental strain. This new strain generated 9.2% (v/v) ethanol (72 g/L) from 20% (w/v) raw corn starch and consumed 75% of the raw starch content during the same period.
Keywords
ethanol production; industrial Saccharomyces cerevisiae; raw starch;
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