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http://dx.doi.org/10.7845/kjm.2013.3035

Isolation of Bacillus atrophaeus MPL-01 from A Wild Boar and Characterization of Its Antifungal Activity  

Yun, Sung-Jo (MPL)
Rho, Jae-Young (School of Bio-science, Dankook University)
Publication Information
Korean Journal of Microbiology / v.49, no.2, 2013 , pp. 195-199 More about this Journal
Abstract
A bacterial strain MPL-01 was isolated from the large intestine of a wild boar. The strain was shown to have morphological, physiological and biochemical characteristics, fatty acids composition typical of Bacillus. The 16S rRNA gene sequence showed that the isolate formed distinct phyletic line that was most closely related to this of Bacillus atrophaeus (99.99%). It was proposed that the strain is classified as B. atrophaeus MPL-01. The strain MPL-01 exhibited the strongest antifungal activity against Colletotrichum acutatum, the pathogen of anthracnose of chili peppers. The ethyl acetate extract of culture filtrate possessed not only the antifungal activity but also the bio-surfactant activity. Therefore, the strain MPL-01 could be a useful bacterium in the development of bio-control process against the pathogenic fungi.
Keywords
Bacillus atrophaeus; 16S rRNA; antifungal activity; biocontrol; wild boar;
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