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http://dx.doi.org/10.7845/kjm.2013.011

In Vitro Construction and Characterization of the Bacteriophage P4 Derivative, P4 sid71 cosP2, Containing the Bacteriophage P2 cos Region  

Kim, Kyoung-Jin (Department of Biomedical Sciences, College of Health Sciences, Sun Moon University)
Publication Information
Korean Journal of Microbiology / v.49, no.1, 2013 , pp. 99-104 More about this Journal
Abstract
Bacteriophage P2 sir mutants are inefficient helpers for their satellite bacteriophage P4. The term, "P2 sir-associated helper inefficiency" has been used to define this phenomenon and it has been suggested that the DNA sequence difference between the cos region of P2 and that of P4 is responsible. To test this hypothesis, P4 derivative phage, P4 sid71 cosP2, containing the cos region of P2 and sid71 allele was constructed through several in vitro DNA manipulation steps. Its burst size was determined using a one-step growth experiment. The results showed that the substitution of the cos region of P2 for the cos region of P4 in P4 sid71 cosP2 overcame "P2 sir-associated helper inefficiency". P4 sid71 cosP2 stock phages prepared with P2 wild type helper and P2 sir helper were analyzed using a CsCl buoyant equilibrium density gradient experiment. The results revealed that the phage particles containing three copies of the P4 genome were the predominant particles in both cases.
Keywords
Escherichia coli; bacteriophage P2-P4; cos region; packaging; P2 sir;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Kim, K-J. 2003. Isolation and identification of the bacteriophage P4 mutant, P4 ost2, suppressing sir mutations of bacteriophage P2. Kor. J. Microbiol. 39, 277-282.   과학기술학회마을
2 Kim, K.J. and Song, J. 2006. Isolation and chracterization of the smallest bacteriophage P4 derives packaged into P4-size head in bacteriophage P2-P4 system. J. Microbiol. 44, 530-536.
3 Kim, K-J., Sunshine, M.G., and Six, E.W. 1998. Characterization and identification of the bacteriophage P4 mutant suppressing sir mutations of bacteriophage P2. J. Microbiol. 36, 262-265.
4 Lin, C-S. 1984. Nucleotide sequence of the essential region of bacteriophage P4. Nucleic Acids Res. 12, 8667-8684.   DOI
5 Lindqvist, B.H. 1971. Vegetative DNA of temperate coliphage P2. Mol. Gen. Genet. 110, 178-196.
6 Marrvik, O.J., Dokland, T., Hanoa, R.N., Jacobse, E., Larson, T., and Lindqvist, B.H. 1995. The capsid size determining protein Sid forms an external scaffold on phage P4 procapsids. J. Mol. Biol. 251, 59-75.   DOI   ScienceOn
7 Nilssen, O., Six, E.W., Sunshine, M.G., and Lindqvist, B.H. 1996. Mutational analysis of the bacteriophage P4 capsid-size-determining gene. Virology 219, 432-442.   DOI   ScienceOn
8 Pruss, G.J., Wang, J.C., and Calendar, R. 1975. In vitro packaging of covalently-closed circular monomers of bacteriophage DNA. J. Mol. Biol. 98, 465-478.   DOI
9 Sasaki, I. and Bertani, G. 1965. Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J. Gen. Microbiol. 40, 365-376.   DOI   ScienceOn
10 Shore, D., Deho, G., Tsipis, J., and Goldstein, R. 1978. Determination of capsid size by satellite bacteriophage P4. Proc. Natl. Acad. Sci. USA 75, 400-404.   DOI   ScienceOn
11 Six, E.W., Sunshine, M.G., Williams, J., Haggard-Ljungquist, E., and Lindqvist, B.H. 1991. Morphoietic switch mutations of bacteriophage P2. Virology 182, 34-46.   DOI   ScienceOn
12 Song, J. and Kim, K-J. 2006. Analysis of DNA conformation in the particles of bacteriophage P4 mutant, P4 ash8 sid71. Kor. J. Microbiol. 42, 62-66.
13 Ziermann, R. and Calendar, R. 1990. Characterization of the cos sites of bacteriophages P2 and P4. Gene 96, 9-15.   DOI   ScienceOn
14 Bertani, L.E. and Six, E.W. 1988. The P2-like phages and their parasite, P4, pp. 73-143. In Calendar, R. (eds.), The bacteriophages, vol. 2. Plenumm Press, New York, USA.
15 Dearborn, A.D., Larurinmaki, P., Chandramouli, P., Rodenburg, C.M., Wang, S., Butcher, S.J., and Dokland, T. 2012. Structure and size determination of bacteriophage P2 and P4 procapsids: Function of size responsiveness mutations. J. Struct. Biol. 178, 215-224.   DOI   ScienceOn