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http://dx.doi.org/10.7845/kjm.2013.016

Biodegradation of Chlorpyrifos (CP) by a Newly Isolated Naxibacter sp. Strain CY6 and Its Ability to Degrade CP in Soil  

Kim, Chul Ho (Institute of Fusion Biotechnology, Gyeongnam National University of Science and Technology)
Choi, Jin Sang (Institute of Fusion Biotechnology, Gyeongnam National University of Science and Technology)
Jang, In Surk (Institute of Fusion Biotechnology, Gyeongnam National University of Science and Technology)
Cho, Kye Man (Institute of Fusion Biotechnology, Gyeongnam National University of Science and Technology)
Publication Information
Korean Journal of Microbiology / v.49, no.1, 2013 , pp. 83-89 More about this Journal
Abstract
A bacterium, isolated from a vegetable field in a plastic film house and named strain CY6 was capable of biodegrading chlorpyrifos (CP). Based on the phenotypic features and the phylogenetic similarity of 16S rRNA gene sequences, strain CY6 was identified as a Naxibacter sp.. CP was utilized as the sole source of carbon and phosphorus by Naxibacter sp. CY6. We examined the role of this Naxibacter sp. in the degradation of other OP insecticides under liquid cultures. Parathion, methyl parathion, diazinon, cadusafos, and ethoprop could also be degraded by Naxibacter sp. CY6 when they are provided as the sole sources of carbon and phosphorus. Additionally, Naxibacter sp. CY6 ($10^8$ CFU/g) added to soil with CP (100 mg/kg) resulted in a higher degradation rate of approximately 90% than the rate obtained from uninoculated soils. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide soil.
Keywords
${\beta}$-proteobacteria; Naxibacter sp. CY6; biodegradation; chlorpyrifos; organophosphours pesticides;
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1 Singh, B.K., Walker, A., Morgan, J.A.W., and Wright. D.J. 2004. Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appl. Environ. Microbiol. 70, 4855-4863.   DOI   ScienceOn
2 Wang, S., Zhang, C., and Yan, Y. 2012. Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12. Biodegradation 23, 107-116.   DOI
3 Xu, P., Li, W.J., Tang, S.K., Zhang, Y.Q., Chen, G.Z., Chen, H.H., Xu, L.H., and Jiang, C.L. 2005. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int. J. Syst. Evol. Microbiol. 55, 1149-1153.   DOI   ScienceOn
4 Xu, G., Zheng, W., Li, Y., Wang, S., Zhang, J., and Yan, Y. 2008. Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by a newly isolated Paracoccus sp. strain TRP. Int. Biodeterior. Biodegrad. 62, 51-56.   DOI   ScienceOn
5 Yang, C., Liu, N., Guo, X., and Qiao, C. 2006. Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil. FEMS Microbiol. Lett. 265, 118-125.   DOI   ScienceOn
6 Yang, J., Yang, C., Jiang, H., and Qiao, C. 2008. Overexpression of methyl parathion hydrolase and its application in detoxification of organophosphates. Biodegradation 19, 831-839.   DOI   ScienceOn
7 Yang, L., Zhao, Y.H., Zhang, B.X., Yang, C.H., and Zhang, X. 2005. Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium. FEMS Microbiol. Lett. 251, 67-73.   DOI   ScienceOn
8 Zhang, R., Cui, Z., Jiang, J., He, J., Gu, X., and Li, S. 2005. Diversity of organophophorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes. Can. J. Micrboiol. 51, 337-343.   DOI   ScienceOn
9 Zhang, Z., Hong, Q., Xu, J., Zhang, X., and Li, S. 2006. Isolation of fenitrothion-degrading strain Burkholderia sp. FDS-1 and cloning of mpd gene. Biodegradation 17, 275-283.   DOI
10 Fu, G., Cui, Z., Huang, T., and Li, S. 2004. Expression, purification, and characterization of novel methyl parathion hydrolase. Protein Expr. Purif. 36, 170-176.   DOI   ScienceOn
11 Islam, S.M.D., Math, R.K., Cho, K.M., Lim, W.J., Hong, S.Y., Kim, J.M., Yun, M.G., Cho, J.J., and Yun, H.D. 2010. Organophosphorus hydrolase (OpdB) of Lactobacillus brevis WCP902 from kimchi is able to degrade organophosphorus pesticides. J. Agric. Food Chem. 58, 5380-5386.   DOI   ScienceOn
12 Kertesz, M.A., Cook, A.M., and Leisinger, T. 1994. Microbial metabolism of sulfur and phosphorus-containing xenobiotics. FEMS Microbiol. Rev. 15, 195-215.   DOI   ScienceOn
13 Li, X., He, J., and Li, S. 2007. Isolation of a chlorpyrifos-degrading bacterium, Sphingomonas sp. strain Dsp-2, and cloning of the mpd gene. Res. Microbiol. 158, 143-149.   DOI   ScienceOn
14 Liu, H., Zhang, J.J., Wang, S.J., Zhang, Z.E., and Zhou, N.Y. 2005. Plasmid-borne catabolism of methyl parathion and p-nitrophenol in Pseudomonas sp. strain WBC-3. Biochem. Biophys. Res. Commun. 334, 1107-114.   DOI   ScienceOn
15 Maya, K., Shigh, R.S., Upadhyay, S.N., and Dubey, S.K. 2011. Kinetic analysis reveals bacterial efficacy for biodegradation of chlorpyrifos and its hydrolyzing metabolite TCP. Proc. Biochem. 46, 2130-2136.   DOI   ScienceOn
16 Molina, L., Ramos, C., Duque, E., Ronchel, M.C., Garcia, J.M., Wyke, L., and Ramos, J.L. 2000. Survival of Pseudomonas putida KT2440 in soil and in the rhizophere of plants under greenhouse and environmental conditions. Soil Biol. Biochem. 32, 316-321.
17 Anwar, S., Liaquat, F., Khan, Q.M., Khalid, Z.M., and Iqbal, S. 2009. Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol by Bacillus pumilus strain C2A1. J. Hazard Mat. 168, 400-405.   DOI   ScienceOn
18 Racke, K.D., Laskowski, D.A., and Schultzon, M.R. 1990. Resistance of chlorpyrifos to enhanced biodegradation in soil. J. Agric. Food Chem. 38, 1430-1436.   DOI
19 Cho, K.M., Math, R.K., Islam, S.M.A., Lim, W.J., Hong, S.Y., Kim, J.M., Yun, M.G., Cho, J.J., and Yun, H.D. 2009. Biodegradation of chlorpyrifos by lactic acid bacteria during kimchi fermentation. J. Agric. Food Chem. 57, 1882-1889.   DOI   ScienceOn
20 Mulbry, W.W., Karns, J.S., Kearney, P.C., Nelson, J.O., McDaniel, C.S., and Wild, J.R. 1986. Identification of a plasmid-borne parathion hydrolase gene from Flavobacterium sp. by Southern hybridization with opd from Pseudomonas diminuta. Appl. Environ. Microbiol. 51, 926-930.
21 Rani, N.L. and Lalitha-Kumari, D. 1994. Degradation of methyl parathion by Pseudomonas putida. Can. J. Microbiol. 4, 1000-1004.
22 Rosenberg, A. and Alexander, M. 1979. Microbial cleavage of various organophosphorus insecticides. Appl. Environ. Microbiol. 37, 886-891.
23 Sardar, D. and Kole, R.K. 2005. Metabolism of chlorpyrifos in relation to its effect on the availability of some plant nutrients in soil. Chemosphere 61, 1273-1280.   DOI   ScienceOn
24 Sethunathan, N.N. and Yoshida, T. 1973. A Flavobacterium that degrades diazinon and parathion. Can. J. Microbiol. 19, 873-875.   DOI   ScienceOn
25 Shelton, D.R. 1988. Mineralization of diethylthiophosphoric acid by an enriched consortium form cattle dip. Appl. Environ. Microbiol. 54, 2572-2573.