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http://dx.doi.org/10.7845/kjm.2012.030

Characterization of a Chitinase Gene and Screening of Cold Active Chitinase from Polar Microorganisms  

Park, Yu Kyung (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Kim, Jung Eun (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Lee, Hyoungseok (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Kim, Ji Hyun (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Park, Ha Ju (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Kim, Dockyu (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Park, Mira (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Yim, Joung Han (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Kim, Il-Chan (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
Publication Information
Korean Journal of Microbiology / v.48, no.4, 2012 , pp. 293-297 More about this Journal
Abstract
Of the 169 strains of microorganisms stored in Polar and Alpine Microbial Collection of Korea Polar Research Institute, 27 strains were selected for their chitinase activity on ZoBell plates supplemented with 0.4% colloidal chitin. Among them, PAMC 21693 strain have shown the highest chitinolytic enzyme activity toward pNP-$(GlcNAc)_1$ at low temperature and the highest growth rate at $4^{\circ}C$. We cloned a full-length chitinase gene of 2,857 bp which contains an open reading frame of 2,169 bp encoding 872-amino acid polypeptide. Recombinant chitinase protein was expressed in E. coli and its molecular weight was confirmed 96 kDa. In this paper, we suggest the potential use of cold-active chitinase from polar microorganisms in the field of biotechnology.
Keywords
chitinase; cold-active chitinase; iPCR; polar microorganisms; screening;
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