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http://dx.doi.org/10.7845/kjm.2012.48.2.141

Cloning and Characterization of Xylanase Gene from Paenibacillus woosongensis  

Yoon, Ki-Hong (Department of Food Science and Biotechnology, Woosong University)
Publication Information
Korean Journal of Microbiology / v.48, no.2, 2012 , pp. 141-146 More about this Journal
Abstract
A gene encoding the xylanase (XynA) predicted from partial genomic sequence of Paenibacillus woosongensis was cloned into Escherichia coli by PCR. This xynA gene consisted of 633 nucleotides, encoding a polypeptide of 211 amino acid residues. The deduced amino acid sequence exhibited 85-89% identity with those of several Paenibacillus xylanases, belonging to the glycosyl hydrolase family 11. As a results of expression of the structural gene by T7 promoter of a pET23a(+) expression vector, xylanase activity was higher in cell-free extract than culture filtrate of a recombinant Escherichia coli BL21(DE3) CodonPlus. However, the expression level of xylanase was not sufficient be detected by SDS-PAGE. The cell-free extract showed maximal xylanase activity at $60^{\circ}C$ and pH 5.5. The predominant products resulting from xylan and xylooligosaccharide hydrolysis were xylose and xylotriose. The enzyme could hydrolyze xylooligosaccharides larger than xylbiose.
Keywords
Paenibacillus woosongensis; cloning; reaction property; xylanase;
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