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http://dx.doi.org/10.7845/kjm.2012.48.1.048

Production Properties on Extracellular Protease from Chryseobacterium Novel Strain JK1  

Lee, Yu-Kyong (Department of Microbiology, Chungbuk National University)
Oh, Yong-Sik (Department of Microbiology, Chungbuk National University)
Roh, Dong-Hyun (Department of Microbiology, Chungbuk National University)
Publication Information
Korean Journal of Microbiology / v.48, no.1, 2012 , pp. 48-51 More about this Journal
Abstract
A novel Chryseobacterium sp. JK1 strain producing extracellular protease had been isolated from soil. The largest clear zones were observed on nutrient agar plates supplemented with 1% skim milk at $30-35^{\circ}C$ along with the growth of Chryseobacterium sp. JK1. The cell growth of JK1 strain was maximal at 24 h and maximum protease activity was reached up to 560 unit/ml at the stationary phase in liquid culture. In the presence of maltose, glucose or mannitol in Nutrient broth, cells grew well, but protease were produced poorly with lower production yields of 64-77% than in NB broth only. Similarly, the addition of skim milk, beef extract, yeast extract, malt extract or tryptone showed good growth and poor enzyme production. On the contrary, the addition of $(NH_4)_2HPO_4$ or $(NH_4)_2SO_4$ gave poor growth and good enzyme production of 121-146%.
Keywords
Chryseobacterium sp. JK1; production condition; protease;
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