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Action Pattern of Anti-Yeast Substance Originated from Rahnella aquatilis Strain AY2000  

Park, Hae-Ji (Department of Life Science and Biotechnology, Dong-Eui University)
Kang, Min-Jung (Department of Life Science and Biotechnology, Dong-Eui University)
Lee, Jong-Hwan (Department of Biotechnology and Bioengineering, Dong-Eui University)
Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University)
Publication Information
Korean Journal of Microbiology / v.47, no.2, 2011 , pp. 163-166 More about this Journal
Abstract
For reduction of side effects by anti-fungal agents, a less toxic anti-fungal substance or a synergistic substance with a new mechanism is needed. The anti-yeast substance (AYS) originated from Rahnella aquatilis strain AY2000 is like to be a heterogeneous protein. The AYS inhibited the growth of Candida albicans in culture broth, and AYS-treated cells were arrested in each phase during cell cycle. Among AYS-treated cells, the population of the cells belonging to sub-G1 phase was not increased during cell cycle. Therefore, AYS has rather yeaststatic than yeastcidal effect to C. albicans. Moreover, with combination of itraconazole or fluconazole, AYS had a synergistic anti-yeast activity against Saccharomyces cerevisiae based on the analysis of fractional inhibitory concentration index.
Keywords
Rahnella aquatilis; anti-fungal substance; anti-yeast; cell cycle; synergistic activity;
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