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Isolation and Characteristics of Endolichenic Fungi Producing Antifungal Compound  

Hwang, Hyun-Gook (Department of Biological Sciences, Sunchon National University)
Kim, Yi-Na (Department of Biological Sciences, Sunchon National University)
Baik, Keun-Sik (Department of Biological Sciences, Sunchon National University)
Choi, Sang-Ki (Department of Biological Sciences, Sunchon National University)
Publication Information
Korean Journal of Microbiology / v.47, no.1, 2011 , pp. 97-101 More about this Journal
Abstract
To isolate a novel antifungal compound, we obtained 107 kinds of endolichenic fungi from Lichen Bioresources Center and examined their antifungal capability. Two fungi EL123 and EL156 showed high antifungal activity against Candida albicans in both MYA and EMM media. Nucleotide sequence analysis and NCBI Blast analysis in ITS region including 5.8S rRNA revealed that EL123 has 95% homology with Thielavia microspora and EL156, 99% with Cryptosporiopsis diversispora which belong to Ascomycetes. It observed that EL156 formed a branched mycelium whereas EL123 formed a straight one. EL156 also produced the antifungal substance faster than EL123 when they grew on MY liquid medium.
Keywords
5.8S rRNA; Cryptosporiopsis diversispora; Thielavia microspora; antifungal; endolichenic fungi;
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1 Arnold, A.E. 2007. Understanding the diversity of foliar fungal endophytes: progress, challenges, and frontiers. Fungal Biol. Rev. 21, 51-66.   DOI   ScienceOn
2 Dams, E., L. Hendriks, Y. Van de Peer, J.M. Neefs, G. Smits, I. Vandenbempt, and R. De Wachter. 1988. Compilation of small rinbosomal subunit RNA sequences. Nucleic Acids Res. 16(Sup.), r87-r173.   DOI
3 Ding, G., Y. Li., S. Fu, S. Liu, J. Wei, and Y.J. Che. 2009. Ambuic acid and torreyanic acid derivatives from the endolichenic fungus Pestalotiopsis sp. Nat. Prod. 72, 182-186.   DOI   ScienceOn
4 Dorn, R.I. and T.M. Oberlander. 1981. Microbial origin of desert varnish. Science 213, 1245-1247.   DOI
5 Gardes, M. and T.D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts. Mol. Ecol. 2, 113-118.   DOI   ScienceOn
6 Hale, M.E. 1983. The Biology of Lichens. 3rd ed. Contemporary Biology Series, Baltimore, USA.
7 Huneck, S. and I. Yoshimura. 1996. Identification of Lichen Products. Springer Verlag, Berlin, Germany.
8 Paranagama, P.A., E.M.K. Wijeratne, A.M. Burns, M.T. Marron, M.K. Gunatilaka, A.E. Arnold, and A.A.L. Gunatilaka. 2007. Heptaketides from Corynespora sp. inhabiting the Cavern Beard lichen, Usnea cavernosa: first report of metabolites of an endolichenic fungus. J. Nat. Prod. 70, 1700-1705.   DOI   ScienceOn
9 Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.