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Effects of spTho1 Deletion and Over-Expression on mRNA Export in Fission Yeast  

Cho, Ye-Seul (Basic Science Research Institute, School of Biological Science and Chemistry, College of Natural Sciences, Sungshin Women's University)
Yoon, Jin-Ho (Basic Science Research Institute, School of Biological Science and Chemistry, College of Natural Sciences, Sungshin Women's University)
Publication Information
Korean Journal of Microbiology / v.46, no.4, 2010 , pp. 401-404 More about this Journal
Abstract
Tho1 is a RNA-binding protein that assembles co-transcriptionally onto the nascent mRNA and is thought to be involved in mRNP biogenesis and mature mRNA export to cytoplasm in budding yeast. In fission yeast Schizosaccharomyces pombe, a homologue of THO1 (spTho1) was identified based on sequence alignment. A deletion mutant in a diploid strain was constructed by replacing one of spTho1-coding region with an ura4+ gene using one-step gene disruption method. Tetrad analysis showed that the spTho1 was not essential for growth. The spTho1 mutant did not show any defects of bulk mRNA export. However, over-expression of spTho1 from strong nmt1 promoter caused the growth defects and accumulation of poly(A)$^+$ RNA in the nucleus. These results suggest that spTho1 is involved in mRNA export from the nucleus to cytoplasm though it is not essential.
Keywords
knock-out mutant; mRNA export; over-expression; S. pombe; spThp1 gene;
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