Browse > Article

A Comparison of Bacterial Diversity Associated with the Sponge Spirastrella abata Depending on RFLP and DGGE  

Jeong, Eun-Ji (Department of Biotechnology, Hannam University)
Im, Choon-Soo (Department of Biotechnology, Hannam University)
Park, Jin-Sook (Department of Biotechnology, Hannam University)
Publication Information
Korean Journal of Microbiology / v.46, no.4, 2010 , pp. 366-374 More about this Journal
Abstract
Culture-dependent RFLP and culture-independent DGGE were employed to investigate the bacterial community associated with the marine sponge Spirastrella abata. A total of 164 bacterial strains associated with the sponge were cultivated using Zobell and Natural sea salt media. PCR amplicons of the 16S rDNA from the bacterial strains were digested with the restriction enzymes HaeIII and MspI, and then assigned into different groups according to their restriction patterns. The 16S rDNA sequences derived from RFLP patterns showed more than 95% similarities compared with known bacterial species, and the isolates belonged to four phyla, Proteobacteria (Alphaproteobacteria, Gammaproteobacteria), Actinobacteria, Firmicutes, and Bacteriodetes, of which Alphaproteobacteria was dominant. DGGE fingerprinting of 16S rDNAs amplified from the sponge- derived total gDNA showed five major DGGE bands, and their sequences showed more than 96% similarities compared with available sequences. The sequences derived from DGGE bands revealed high similarity with the uncultured bacterial clones. DGGE revealed that bacterial community consisted of four phyla, including Proteobacteria (Alphaproteobacteria, Gammaproteobacteria), Actinobacteria, Spirochetes, and Chloroflexi. Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria were commonly found in bacteria associated with S. abata by both RFLP and DGGE methods; however, overall bacterial community in the sponge differed depending on the analysis methods.
Keywords
DGGE fingerprinting; RFLP; Spirastrella abata; sponge-associated bacteria;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Li, Z.Y., L.M. He, J. Wu, and Q. Jiang. 2006. Bacterial community diversity associated with four marine sponges from the South China Sea based on 16S rDNA-DGGE fingerprinting. J. Exp. Mar. Biol. Ecol. 329, 75-85.   DOI   ScienceOn
2 Mohamed, N.M., V. Rao, M.T. Hamann, M. Kelly, and R.T. Hill. 2008. Monitoring bacterial diversity of the marine sponge Ircinia strobilina upon transfer into aquaculture. Appl. Environ. Microbiol. 74, 4133-4143.   DOI   ScienceOn
3 Enticknap, J.J., M. Kelly, O. Peraud, and R.T. Hill. 2006. Characterization of a culturable alphaproteobacterial symbiont common to many marine sponges and devidence for vertical transmission via sponge Larvae. Appl. Environ. Microbiol. 72, 3724-3732.   DOI   ScienceOn
4 Salmoun, M., C. Devijver, D. Daloze, J.C. Breakman, R. Gomez, M. de Kluijver, and R.W.M. Van Soest. 2000. New sesquiterpene/ Quinones from two sponges of the genus Hyrtios. J. Nat. Prod. 63, 452-456.   DOI   ScienceOn
5 Zhang, H., Y.K. Lee, W. Zhang, and H.K. Lee. 2006. Culturable actinobacteria from the marine sponge Hymeniacidon perleve: isolation and phylogenetic diversity by 16S rRNA gene-RFLP analysis. Antonie van Leeuwenhoek 90, 159-169.   DOI   ScienceOn
6 Thiel, V., S. Leininger, R. Schamljohann, F. Brümer, and J. Imhoff. 2007. Sponge-specific bacterial associations of the Mediterranean sponge Chondrilla nucula (Demospongiae, tetractinomorpha). Microbial. Ecol. 54, 101-111.   DOI   ScienceOn
7 Thiel, V., S.C. Neulinger, T. Saufenberger, R. Schmaljohann, and J.F. Imhoff. 2007. Spatial distribution of sponge-associated bacteria in the Medittanean sponge Tethya aurantium. FEMS Microbiol. Ecol. 59, 47-63.   DOI   ScienceOn
8 Thomson, C., M. Horn, W. Wagner, U. Hentschel, and P. Proksch. 2003. Monitoring microbial diversity and natural products profiles of the sponge Aplysina cavericola following transplantation. Mar. Biol. 142, 685-692.   DOI
9 Webster, N.S., A.P. Negri, M.M. Munro, and C.N. Battershill. 2004. Diverse microbial communities inhabit Antarctic sponges. Environ. Microbiol. 6, 288-300.   DOI   ScienceOn
10 Ridley, C.P., D.J. Faulkner, and M.G. Haygood. 2005. Investigation of Oscillatoria spongeliae-dominated bacterial communities in four dictyoceratid sponges. Appl. Environ. Microbiol. 71, 7366-7375.   DOI   ScienceOn
11 Park, S.H., K.K. Kwon, D.S. Lee, and H.K. Lee. 2002. Morphological diversity of marine microorganisms on different isolation media. J. Micorobiol. 40, 161-165.
12 Shin, B.A., Y.R. Kim, I.S. Lee, C.K. Sung, J.K. Hong, C.J. Sim, K.S. Im, and J.H. Jung. 1999. Lyso-PAF analogues and lysophosphatidylcholines from the marine sponge Spirastrella abata as inhibitors of cholesterol biosyntehsis. J. Nat. Prod. 62, 1554-1557.   DOI   ScienceOn
13 Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA 4. Mol. Biol. Evol. 24, 1596-1599.   DOI   ScienceOn
14 Muscholl-Silberhorn, A., V. Thiel, and J.F. Ihoff. 2008. Abundance and bioactivity of cultured sponge-associated bacteria from the Mediterranean Sea. Microbial. Ecol. 55, 94-106.   DOI   ScienceOn
15 Park, J.S. 2010. Bacterial community diversity associated with two marine sponges from the South Pacific Ocean based on 16S rDNA-DGGE analysis. Kor. J. Microbiol. 46, 177-182.
16 Park, J.S., J.J. Sim, and K.D. An. 2009. Community structure of bacteria associated with two marine sponges from Jeju Island based on 16S rDNA-DGGE profile. Kor. J. Microbiol. 45, 170-176.   과학기술학회마을
17 Radwan, M., A. Hanora, J. Zan, N.M. Mohamed, D.M. Abo-Elamatty, S.H. Abou-El-Ela, and R.T. Hill. 2009. Bacterial community analyses of two Red Sea sponges. Mar. Biotechnol. 12, 350-360.
18 Levina, E.V., A.I. Kalinovsky, P.V. Andriyashenko, P.S. Dmitrenok, D.L. Aminin, and V.A. Stonik. 2005. Phrygiasterol, a cytotoxic cyclopropane-containing polyhydroxysteroid, and related compounds from the pacific starfish Hippasteria phrygiana. J. Nat. Prod. 68, 1541-1544.   DOI   ScienceOn
19 Li, Z., L. He, and X. Miao. 2007. Cultivable bacterial community from South China Sea sponge as revealed by DGGE fingerprinting and 16S rDNA phylogenetic analysis. Curr. Microbiol. 55, 465-472.   DOI   ScienceOn
20 Li, Z., Y. Hu, Y. liu, Y. Huang, L. He, and X. Miao. 2007. 16S rDNA clone library based bacterial phylogenetic diversity associated with three South China Sea sponges. World J. Microbiol. Biotechnol. 23, 1265-1272.   DOI   ScienceOn
21 Li, Z.Y. and Y. Liu. 2006. Marine sponge Craniella austrialiensis- associated bacterial diversity revelation based on 16S rDNA library and biologically active Actinomycetes screening, phylogenetic analysis. Lett. Appl. Microbiol. 43, 410-416.   DOI   ScienceOn
22 Cho, H.H. and J.S. Park. 2009. Comparative analysis of the community of culturable bacteria associated with sponges, Spirastrella abata and Spirastrella panis by 16S rDNA-RFLP. Kor. J. Microbiol. 45, 155-162.   과학기술학회마을
23 Hooper, N.J.A. and R.W.M. van Soest. 2002. Systema Porifera: A guide to the classification of sponges. In N. BouryEsnault and C. Donadey (eds.). Kluwer Academic/Plenum Publisher, New York, USA.
24 Cho, H.H., E.J. Sim, and J.S. Park. 2010. Phylogenetic diversity of bacteria associated with the marine sponge, Spirastrella abata and Cinachyrella sp. Kor. J. Microbiol. 46, 177-182.
25 Hardoim, C.C., R. Costa, F.V. Araujo, E. Hajdu, R. Peixoto, U. Lins, A.S. Rosado, and J.D. van Elsas. 2009. Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters. Appl. Environ. Microbiol. 75, 3331-3343.   DOI   ScienceOn
26 Hentschel, U., J. Hopke, M. Horn, A.B. Friedrich, M. Wagner, J. Hacker, and B.S. Moore. 2002. Molecular evidence for a uniform microbial community in sponge from different oceans. Appl. Environ. Microbiol. 68, 4431-4440.   DOI   ScienceOn
27 Ko, S.R., S.J. Park, C.Y. Ahn, A. Choi, J.S. Lee, H.S. Kim, B.D. Yoon, and H.M. Oh. 2004. Analysis of microbial communities during cyanobacterial bloom in Daechung Reservoir by DGGE. Kor. J. Microbiol. 40, 205-210.   과학기술학회마을
28 Alam, N., W. Wang, J.K. Hong, C.O. Lee, K.S. Im, and J.H. Jung. 2002. Cytotoxic sphingosine 4-sulfates from the sponge Spirastrella abata. J. Nat. Prod. 65, 944-945.   DOI   ScienceOn
29 Alam, N., B.H. Bae, J. Hong, C.O. Lee, B.A. Shin, K.S. Im, and J.H. Jung. 2001. Additional bioactive Lyso-PAF congeners from the sponge Spirastrella abata. J. Nat. Prod. 64, 533-535.   DOI   ScienceOn