Browse > Article

Antimicrobial Susceptibility of Staphylococci sp. Isolated from Bovine Milk  

Kim, Ji-Hoon (Division of Animal Science, Sahmyook University)
Ko, Mun-Joo (Division of Animal Science, Sahmyook University)
Kim, Ka-Hee (Division of Animal Science, Sahmyook University)
Lee, Seung-Hoon (Division of Animal Science, Sahmyook University)
Choi, Sung-Sook (College of Pharmacy, Sahmyook University)
Publication Information
Korean Journal of Microbiology / v.46, no.4, 2010 , pp. 341-345 More about this Journal
Abstract
The prevalence and antimicrobial susceptibilities of Staphylococcal isolates from bovine milk samples were assessed. From January 2009 to October 2009, a total 287 bovine milk samples were randomly collected from 15 stock raising farms located in northern area of Kyunggi province and cultured for the presence of Staphylococci spp. A total 79 staphylococcal isolates were recovered from the milk samples. The predominant isolates were S. aureus (43.03%) and S. chromogenes (24.05%). Antimicrobial resistance patterns of 79 Staphylococcal isolates against ampicillin, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, oxacillin, teicoplanin, tetracyclin, and vancomycin were tested. Staphylococcal isolates revealed the highest resistance to ampicillin (56.96%) and oxacillin (39.23%). Of 31 oxacillin resistance strains, 8 strains carry mecA gene which is responsible for methicillin resistance.
Keywords
antimicrobial susceptibilities; bovine milk sample; mecA; Staphylococcal isolates;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Rajala-Schultz, P.J., A.H. Torres, F.J. Degraves, W.A. Gebreyes, and P. Patchanee. 2009. Antimicrobial resistance and genotypic characterization of coagulase-negative staphylococci over the dry period. Vet. Micobiol. 134, 55-64.
2 Sampimon, O.C., J.C. Vernooij, D.J. Mevius, and J. Sol. 2007. Sensitivity to various antibiotics of coagulase-negative staphylococci isolated from samples of milk from Dutch dairy cattle. Tijdschr Diergeneeskd 132, 200-204.
3 Sawant A.A., B.E. Gillespie, and S.P. Oliver. 2009. Antimicrobial susceptibility of coagulase-negative Staphylococcus species isolated from bovine milk. Vet. Microbiol. 134, 73-81.   DOI   ScienceOn
4 Srinivasan, V., A.A. Sawant, B.E. Gillespie, S.J. Headrick, L. Ceasaris, and S.P. Oliver. 2006. Prevalence of enterotoxin and toxic shock syndrome toxin genes in Staphylococcus aureus isolated from milk of cows with mastitis. Foodborne Pathog. Dis. 3, 274-283.   DOI   ScienceOn
5 Stacey, L.K., L.M. Stanley, N. Mariani, and B. Tom. 2003. The Resistance Phenomenon in Microbes and Infectious Disease Vectors: Implications for Human Health and Strategies for Containment -- Workshop Summary. Appendix D: Executive Summary: WHO Report. pp. 256-273.
6 Woods, G.L. 1995. In vitro testing of antimicrobial agents. Infect. Dis. Clin. North. 9, 463-481.
7 Yoon, J.C., J.C. Lee, S.K. Kim, T.S. Park, J.T. Kim, C.G. Lee, and C.Y. Lee. 2004. Prevalence of isolated microorganisms and antimicrobial susceptibility from half milk in dairy goats. Korea J. Vet. Res. 44, 151-157.
8 Lee, Y.S., C.K. Kim, M.S. Kim, D.G. Yong, K.W. Lee, and Y.S. Chong. 2007. Detection of mecA in strains with oxacillin and cefoxitin disk tests for detection of methicillin-resistant Staphylococcus. Kor. J. Lab. Med. 27, 276-280.   DOI
9 Moon, J.S., A.R. Lee, H.M. Kang, E.S. Lee, Y.S. Joo, Y.H. Park, M.N. Kim, and H.C. Koo. 2007. Antibiogram and coagulase diversity in staphylococcal enterotoxin-producing Staphylococcus aureus from bovine mastitis. J. Dairy Sci. 90, 1716-1724.   DOI   ScienceOn
10 Mathews, A.A., M. Thomas, B. Appalaraju, and J. Jayalakshmi. 2010. Evaluation and comparison of tests to detect methicillin resistant S. aureus. Indian J. Pathol. Microbiol. 53, 79-82.   DOI   ScienceOn
11 NORM. 2003. NORM-VET: Usage of antimicrobial agents and occurrence of antimicrobial resistance in Norway, pp. 1-72.
12 Noskin, G.A., R.J. Rubin, J.J. Schentag, J. Kluytmans, E.C. Hedblom, M. Smulders, E. Lapetina, and E. Gemmen. 2005. Adverse clinical and economic outcomes attributable to methicillin resistance among patients with Staphylococcus aureus surgical site infection. Arch. Intern. Med. 165, 1756-1761.   DOI   ScienceOn
13 OIE. 2001. European Scientific Conference. The use of antibiotics in animal ensuring the protection of public health. pp. 8-142.
14 Oliver, S.P., B.M. Jayarao, and R.A. Almeida. 2005. Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathog. Dis. 2, 15-29.
15 Oliver, S.P., K.J. Boor, S.C. Murphy, and S.E. Murinda. 2009. Food safety hazards associated with consumption of raw milk. Foodborne Pathog. Dis. 26, 793-806.
16 Gentilini, E., G. Denamiel, A. Betancor, M. Rebuelto, M.R. Fermepin, and R.A. De Torrest. 2002. Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis in Argentina. J. Dairy Sci. 85, 1913-1917.   DOI   ScienceOn
17 Ito, T. and K. Hiramatsu. 1998. Acquisition of methicillin resistance and progression of multiantibiotic resistance in methicillin- resistant Staphylococcus aureus. Yunsei Medical Journal. 39, 526-533.   DOI
18 Gillespie, B.E., S.I. Headrick, S. Boonyayatra, and S.P. Oliver. 2009. Prevalence and persistence of coagulase-negative Staphylococcus species in three dairy research herds. Vet. Microbiol. 134, 65-72.   DOI   ScienceOn
19 Hwang, I.G., H.S. Kwak, and S.H. Yoon. 2010. Methicillinresistant Staphylococcus aureus (MRSA) as a foodborne biological hazard. Safe Food. 5, 26-35.
20 Hwang, S.Y., B.Y. Moon, Y.H. Park, M.J. Lee, H.A. Bang, K.H. Rhim, J.S. Kim, N.H. Che, and W.C. Lee. 2010. Comparative study on the epidemiology of food-borne disease outbreaks in Korea and Japan. J. Fd. Hyg. Safety. 25, 129-132.   과학기술학회마을
21 Kim, H.J., Y.H. Park, and M.S. Koo. 2010. Antimicrobial resistance and risk assessment. Safe Food. 5, 14-19.
22 Kuhl, S.A., P.A. Pattee, and N.J. Baldwin. 1978. Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus. J. Bacteriol. 135, 460-465.
23 Bal, E.B., S. Bayar, and M.A. Bal. 2010. Antimicrobial susceptibilities of coagulase-negative staphylococci (CNS) and streptococci from bovine subclinical mastitis cases. J. Microbiol. 48, 267-274.   DOI
24 Engemann, J.J., Y. Carmeli, S.E. Cosgrove, V.G. Fowler, M.Z. Bronstein, S.L. Trivette, J.P. Briggs, D.J. Sexton, and K.S. Kaye. 2003. The burden of Staphylococcus aureus infections on hospitals in the United States: an analysis of the 2000 and 2001 Nationwide Inpatient Sample Database. Clin. Infect. Dis. 36, 592-598.   DOI   ScienceOn
25 CLSI. 2007. Performance standards for antimicrobial susceptibility testing: seventeenth informational supplement, pp. 53-59. Clinical and Laboratory Standards Institute.
26 Codex Aimentarius Commission. 1999. Principles and guidelines for the conduct of microbiological risk assessment, pp. 1-45. CAC/GL30. Geneva.
27 DANMAP. 2003. Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animal, foods and humans in Denmark, pp. 1-85.
28 FAO/OIE/WHO. 2004. Joint FAO/OIE/WHO 2nd workshop on non-human antimicrobial usage and antimicrobial resistance; management options, executive summary, Olso, Norway.
29 Anyliffe, G.A.J. 1997. The progressive intercontinental spread of methicillin-resistant Staphylococcus aureus. Clin. Infect. Dis., 24 (Suppl. 1), 4-49.
30 Anon, K. 1995. Results of the Uruguay round of the multilateral trade negotiations 1993: agreement on application of sanitary and phytosanitary measures. pp. 26-47. World Trade Organisation, Genova.