Browse > Article

Detection of plcR-papR Genes by PCR in Identifying Enterotoxin Genes-Harboring Bacillus cereus Strains  

Yun, Suk-Hyun (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
Kim, Yong-Sang (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
So, Soon-Ku (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
Jeong, Do-Yeon (Institute of Sunchang Fermented Soybean Products)
Hahn, Kum-Su (Institute of Sunchang Fermented Soybean Products)
Uhm, Tai-Boong (Division of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
Publication Information
Korean Journal of Microbiology / v.45, no.4, 2009 , pp. 425-429 More about this Journal
Abstract
Identification of virulent Bacillus cereus strains was examined by PCR using primers specific for the detection of plcR-papR, which encode regulatory proteins controlling the transcription of virulence factors in B. cereus. Total 96 strains of B. cereus that carried at least one of diarrheal toxin genes including hblACD, nheABC, and cytK showed all positive PCR products, while other 48 Bacillus strains that lacked the toxin genes were plcRpapR-negative. This PCR method targeting the plcR-papR genes appears to be simple and effective in identifying the enterotoxin genes-harboring B. cereus strains.
Keywords
B. cereus; enterotoxin genes; papR; PCR primer; plcR;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bouillaut, L., S. Perchat, S. Arold, S. Zorrilla, L. Slamti, C. Henry, M. Gohar, N. Declerck, and D. Lereclus. 2008. Molecular basis for group-specific activation of the virulence regulator PlcR by PapR heptapeptides. Nucleic Acid Res. 36, 3791-3801   DOI   ScienceOn
2 Dietrich, R., M. Moravek, C. Bürk, P.E. Granum, and E. Martlbauer. 2005. Production and characterization of antibodies against each of the three subunits of the Bacillus cereus nonhemolytic enterotoxin complex. Appl. Environ. Microbiol. 71, 8214-8220   DOI   ScienceOn
3 Hansen, B.M. and N.B. Hendriksen. 2001. Detection of enterotoxic Bacillus cereus and Bacillus thuringiensis strains by PCR analysis. Appl. Environ. Microbiol. 67, 185-189   DOI   ScienceOn
4 Ngamwongsatit, P., W. Buasri, P. Pianariyanon, C. Pulsrikarn, M. Ohba, A. Assavanig, and W. Panbangred. 2008. Broad distribution of enterotoxin genes among Bacillus thuringiensis and Bacillus cereus as shown by novel primers. Int. J. Food Microbiol. 121, 352-356   DOI   ScienceOn
5 Rosen, S. and H.J. Skaletsky. 2000. PRIMER3 on the WWW for general users and for biologist programmers, pp. 365-386. In S. Krawetz and S. Misener (eds.), Bioinformatics Methods and Protocols: Method in Molecular Biology, Humana Press, Totowa, NJ, USA
6 Zhang, Z., S. Schwartz, L. Wagner, and W. Miller. 2000. A greedy algorithm for aligning DNA sequences. J. Comput. Biol. 7, 203-214   DOI   ScienceOn
7 Moravek, M., M. Wegscheider, A. Schulz, R. Dietrich, C. Burk, and E. Martlbauer. 2004. Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus. FEMS Microbiol. Lett. 238, 107-113   DOI   PUBMED   ScienceOn
8 Schoeni, J.L. and A.C. Wong. 2005. Bacillus cereus food poisoning and its toxins. J. Food Prot. 68, 636-648   DOI   PUBMED   ScienceOn
9 From, C., R. Pukall, P. Schumann, V. Hormazabal, and P.E. Granum. 2005. Toxin-producing ability among Bacillus spp. outside the Bacillus cereus group. Appl. Environ. Microbiol. 71, 1178-1180   DOI   ScienceOn
10 Yang, I.C., D.Y. Shih, J.Y. Wang, and T.M. Pani. 2007. Development of rapid real-time PCR and most probable number real-time PCR assays to quantify enterotoxigenic strains of the species in the Bacillus cereus group. J. Food Prot. 70, 2774-2781   DOI   PUBMED   ScienceOn
11 Fricker, M., U. Messelhäußer, U. Busch, S. Scherer, and M. Ehling-Schulz. 2007. Diagnostic real-time PCR assays for the detection of emetic Bacillus cereus in foods and recent food-borne outbreaks. Appl. Environ. Microbiol. 73, 1892-1898   DOI   ScienceOn
12 Granum, P.E. and T. Lund. 1997. Bacillus cereus and its food poisoning toxins. FEMS Microbiol. Lett. 157, 223-228   DOI   ScienceOn
13 Ehling-Schulz, M., B. Svensson, M.H. Guinebretiere, T. Lindbäck, M. Andersson, A. Schulz, M. Fricker, A. Christiansson, P.E. Granum, E. Märtibauer, C. Nguyen-The, M. Salkinoja-Salonen, and S. Scherer. 2005. Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains. Microbiology 151, 183-197   DOI   ScienceOn
14 Gohar, M., K. Faegri, S. Perchat, S. Ravnum, O. Okstad, M. Gominet, A. Kolste, and D. Lereclus. 2008. The PlcR virulence regulon of Bacillus cereus. PLoS One 3, 1-9   DOI   ScienceOn
15 윤숙현, 김용상, 정도연, 한금수, 엄태붕. 2009. Mannitol-egg york-polymyxin B 선택배지에서 Bacillus cereus 계수 방법의 재평가. 미생물학회지 45, 208-214   과학기술학회마을