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Efficacy of Genetic Adjuvant (Plasmid-Expressed Chicken Interleukin-6) and Chemical Adjuvant (Levamisole) on the Protective Immunity of Genetic Vaccine against Infectious Bursal Disease Virus  

Park, Jeong-Ho (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Sung, Haan-Woo (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Yoon, Byung-Il (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Pak, Son-Il (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Kwon, Hyuk-Moo (School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 91-98 More about this Journal
Abstract
Infectious bursal disease (IBD) caused by the infectious bursal disease virus (IBDV) has an important economic impact on the poultry industry worldwide. This study examined the adjuvant effects of a plasmid encoding chicken interleukin-6 (pcDNA-ChIL-6) and levamisole (LMS) on in ovo prime-boost vaccination using a genetic vaccine (pcDNA-VP243) to prime in chicken followed by a killed-vaccine boost. A pcDNA-VP243 was injected into the amniotic sac alone or in combination with a pcDNA-ChIL-6 or LMS at embryonation day 18, followed by an intramuscular injection of killed IBD vaccine at 1 week of age. The chicken were orally challenged with very virulent IBDV (vvIBDV) strain at 3 weeks of age and observed for 10 days. No mortality was observed in the groups that received the pcDNA-VP243 alone and pcDNA-VP243 plus pcDNA-ChIL-6 or LMS compared to 100% mortality in unvaccinated challenge control group. However, as determined by bursal damage (the presence of IBDV RNA, B/B ratio, and lesion score), a pcDNA-VP243 alone group was superior to pcDNA-VP243 plus pcDNA-ChIL-6 or LMS groups in the protection against post-challenge. These findings suggest that in ovo priming with genetic vaccine and boosting with killed vaccine is an effective strategy for protecting chicken against vvIBDV and the addition of pcDNA-ChIL-6 or LMS did not enhance protective immunity.
Keywords
chicken interleukin 6; genetic vaccine; in ovo vaccination; infectious bursal disease; levamisole;
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