Browse > Article

Reevaluation of Enumeration of Bacillus cereus Grown on Mannitol-Egg York-Polymyxin B Agar  

Yun, Suk-Hyun (Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
Kim, Yong-Sang (Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
Jeong, Do-Yeon (Institute of Sunchang Fermented Soybean Products)
Hahn, Kum-Su (Institute of Sunchang Fermented Soybean Products)
Uhm, Tai-Boong (Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 208-214 More about this Journal
Abstract
To avoid ambiguity in counting the number of colony, about 1,500 of colonies grown on B. cereus selective agar plates were grouped into 12 types by morphological difference and then identified by biochemical and 16S rDNA nucleotide sequence. Among them, seven colony types with 11 to 15 mm diameters of halo were identified as B. cereus or B. cereus subsp. cytotoxis. Five mm sized colonies with no halo, which have not been considered as B. cereus according to the manufacturer's manual, were identified as B. cereus. A colony type with double halos of only 6 mm in diameter was also B. cereus. Other three types were proven to be Enterococcus sp., Brevibacillus sp., and B. subtilis, respectively. PCR results showed that only 9 types that are identified as B. cereus strains harbor at least one of B. cereus toxin genes.
Keywords
Bacillus cereus; Bacillus cereus selective media; microbial enumeration; microbial identification;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 식품공전. 2008. 식품의약품안전청. 고시 제 2007-38호
2 Apetroaie, C., M.A. Andersson, C. Sproer, I. Tsitko, R. Shaheen, E.L. Jaaskelainen, L.M. Wijnands, R. Heikkila, and M.S. Salkinoinoja-Salonen. 2005. Cereulide-producing strains of Bacillus show diversity. Arch. Microbiol. 184, 141-151   DOI   ScienceOn
3 Holt, J.G., N.R. Krieg, P.H.A. Sneath, and S.T. Williams. 1994. Group 18: Endospore-forming Gram-positive rods and cocci, pp. 559-564. In J.G. Holt, K.H. Schleifer, J.G. Tully, J. Ursing, M. Bryant, N.R. Krieg, J. Liston, J.W. Moulder, R.G.E. Murray, C.F. Niven, and N. Pfenning (eds.), Bergey's manual of systematic bacteriology, 9th ed. The Williams & Wilkins Co., Baltimore, MD, USA.
4 Ouhib, Q., T. Clavel, and P. Schmitt. 2006. The production of Bacillus cereus enterotoxins is influenced by carbohydrate and growth rate. Curr. Microbiol. 53, 222-226   DOI   ScienceOn
5 Guinebretiere, M.H., V. Broussolle, and T. Nguyen-The. 2002. Enterotoxigenic profiles of food-poisoning and food-borne Bacillus cereus strains. J. Clin. Microbiol. 40, 3053-3056   DOI   ScienceOn
6 Rosen, S. and H.J. Skaletsky. 2000. PRIMER3 on the WWW for general users and for biologist programmers, pp. 365-386. In S. Krawetz and S. Misener (eds.), Bioinformatics Methods and Protocols: Method in Molecular Biology, Humana Press, Totowa, NJ, USA.
7 Lindback, T., A. Fagerlund, M.S. Rodland, and P.E. Granum. 2004. Characterization of the Bacillus cereus Nhe enterotoxin. Microbiology 150, 3959-3967   DOI   ScienceOn
8 식품공전. 2008. 식품의약품안전청. 일반 시험법 10-8-40
9 Altayar, M. and A.D. Sutherland. 2005. Bacillus cereus is common in the environment but emetic toxin producing isolates are rare. J. Appl. Microbiol. 100, 7-14   DOI   ScienceOn
10 Birgit, M.P., R. Dietrich, B. Nibler, E. Martlbauer, and S. Scherer. 1999. The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. Appl. Environ. Microbiol. 65, 5436-5442   PUBMED   ScienceOn
11 Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680   DOI   ScienceOn
12 Ngamwongsatit, P., W. Buasri, P. Pianariyanon, C. Pulsrikarn, M. Ohba, A. Assavanig, and W. Panbangred. 2008. Broad distribution of enterotoxin genes among Bacillus thuringiensis and Bacillus cereus as shown by novel primers. Int. J. Food Microbiol. 121, 352-356   DOI   ScienceOn
13 Schoeni, J.L. and A.C. Wong. 2005. Bacillus cereus food poisoning and its toxins. J. Food Prot. 68, 636-648   DOI   PUBMED   ScienceOn
14 Granum, P.E. and T. Lund. 1997. Bacillus cereus and its food poisoning toxins. FEMS Microbiol. Lett. 157, 223-228   DOI   ScienceOn
15 Swofford, D.L. 1998. PAUP*. Phylogenetic Analysis using Parsimony. 4.0 ed. Sinauer Associates, Sunderland, MA, USA.
16 Zhang, Z., S. Schwartz, L. Wagner, and W. Miller. 2000. A greedy algorithm for aligning DNA sequences. J. Comput. Biol. 7, 203-214   DOI   ScienceOn