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Isolation and Identification of Halotolerant Bacillus sp. SJ-10 and Characterization of Its Extracellular Protease  

Kim, Eun-Young (Department of Biotechnology, Pukyong National University)
Kim, Dong-Gyun (Department of Biotechnology, Pukyong National University)
Kim, Yu-Ri (Department of Biotechnology, Pukyong National University)
Choi, Sun-Young (Department of Biological Analysis, Chemical Defense Research Institute)
Kong, In-Soo (Department of Biotechnology, Pukyong National University)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 193-199 More about this Journal
Abstract
A bacterium producing the halotolerant extracellular protease was isolated from squid jeotgal, and was identified as Bacillus sp. SJ-10 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The strain grew at $20^{\circ}C\sim55^{\circ}C$, pH 5~8, and 0%~14% NaCl and optimal growth conditions were $35{\pm}5^{\circ}C$, pH 7, and 5% NaCl. The major cellular fatty acids were anteiso-$C_{15:0}$, anteiso-$C_{17:0}$, and $C_{16:0}$ DNA G+C content was 50.58 mol% and menaquinone consisted of MK-7 Phylogenic analysis based on the 16S rRNA gene sequence indicated that SJ-10T belongs to the genus Bacillus. About 40 kDa of the salt-tolerant protease was purified by 40% ammonium sulfate saturation and Mono Q column chromatography. The optimal activity of the protease was pH 8 and stable at pH 5~10. The optimum temperature and NaCl concentration were $35{\pm}5^{\circ}C$ and $5{\pm}1%$, respectively.
Keywords
Bacillus sp.; collagen degrading enzyme; halophilic bacteria;
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