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Molecular Epidemiological Relationship of the Pathogenicity of Legionella spp. Isolated from Water Systems in Seoul  

Kim, Jin-Ah (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Jung, Ji-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Soo-Jin (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Jin, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Oh, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Han, Gi-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 126-132 More about this Journal
Abstract
Legionella spp. is the causative agent of Legionellosis, which induces a potentially fatal form of pneumonia. With a concentrated performance during the summer of 2008, we secured 73 isolates from the water systems of 25 wards in Seoul. We analysed serotypes, pathogenic genes (Dot/Icm), and patterns of pulsed-field gel electrophoresis (PFGE) in an attempt to confirm relationships among them. Different from the previous year's pattern (2007), among the isolates, 69 were Legionella pneumophila and 4 were Legionella spp. The serotype distribution of Legionella pneumophila was sg1 43, sg6 9, sg5 8, sg3 8, and sg2 1. The serotype for the 4 Legionella spp. was Legionella nautarum. Most of the Legionella pneumophila had several pathogenic genes. On the other hand, the 4 Legionella spp. were defective in pathogenicity in genomic terms. The PFGE patterns of the serotypes showed a tendency for diversity of Legionella pneumophila and a close correlation with genetic pathogenicity.
Keywords
dot/icm; Legionella; pathogenicity; PFGE;
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1 Amemura-Maekawa, J., F. Kura, B. Chang, and H. Watanabe. 2005. Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster. Microbiol. Immunol. 49, 1027-1033   DOI   PUBMED   ScienceOn
2 Denka Seiken Co. 1998. Bacteriology product information. Denka Seiken Co. Ltd.
3 Khler, R., J. Franghnel, B. Knig, E. Lneberg, and M. Frosch. 2003. Biochemical and functional analyses of the Mip protein: Influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of Legionella pneumophila. Infect. Immun. 71, 4389-4397   DOI   ScienceOn
4 Lee, H.K., H.S. Yang, S.R. Hong, M.S. Park, S.K. Sim, B.C. Lee, and M.Y. Park. 2001. Molecular typing of Legionella pneumophila Korean isolates from 1985 to 2001. The Report of National Institute of Health 38, 18-30
5 Molmeret, M., M. Santic, R. Asare, R.A. Carabeo, and Y. Abu-Kwaik. 2007. Rapid escape of the dot/icm Mutants of Legionella pneumophila into the cytosol of mammalian and protozoan cells. Infect. Immun. 75, 3290-3304   DOI   ScienceOn
6 Field, B.S., R.F. Benson, and R.E. Besser. 2002. Legionella and Legionnaires' disease: 25 Years of investigation. Clin. Microbiol. Rev. 15, 506-526   DOI   ScienceOn
7 Fliermans, C.B., W.B. Cherry, L.H. Orrison, S.J. Smith, D.L. Tison, and D.H. Pope. 1981. Ecological distribution of Legionella pneumophila. Appl. Environ. Microbiol. 41, 9-16   PUBMED   ScienceOn
8 Segal, G., M. Purcell, and H.A. Shuman. 1998. Host cell killing and bacterial conjugation required overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome. Proc. Natl. Acad. Sci. USA 95, 1669-1674   DOI   ScienceOn
9 Vogel, J.P., H.L. Andrews, S.K. Wong, and R.R. Isberg. 1998. Conjugative transfer by the virulence system of Legionella pneumophila. Science 279, 873-876   DOI   PUBMED   ScienceOn
10 Zink, S.D., L. Pedersen, N.P. Cianciotto, and Y. Abu-Kwaik. 2002. The Dot/Icm type IV secretion system of Legionella pneumophila is essential for the induction of apoptosis in human macrophages. Infect. Immun. 70, 1657-1663   DOI   ScienceOn
11 Nintasen, R., F. Utrarachkij, K. Siripanichgon, A. Bhumiratana, Y. Suzuki, and O. Suthienkul. 2007. Enhancement of Legionella pneumophila culture isolation from microenvironments by macrophage infectivity potentiator (mip) gene-specific nested polymerase chain reaction. Microbiol. Immunol. 51, 777-785   DOI   PUBMED   ScienceOn
12 Seung, H.J., J.H. Jung, S.J. Kim, Y.H. Jin, S.M. Lee, M.S. Kim, and J.G. Kim. 2007. Molecular epidemiological study of Legionella pneumophila isolated from water systems in Seoul. The Report of Seoul Metropolitan Government Research Institute of Public Health and Environment 43, 283-293
13 Gal-Mor, O., T. Zusman, and G. Segal. 2002. Analysis of DNA regulatory elements required for expression of the Legionella pneumophila icm and dot virulence genes. J. Bacteriol. 184, 3823-3833   DOI   ScienceOn
14 Berger, K.H., J.J. Merriam, and R.R. Isberg. 1994. Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA gene. Mol. Microbiol. 14, 809-822   DOI   ScienceOn
15 Marra, A., S.J. Blander, M.A. Horwitz, and H.A. Shuman. 1992. Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc. Natl. Acad. Sci. USA 89, 9607-9611   DOI   ScienceOn
16 Terry Alli, O.A., S. Zink, N.K. von Lackum, and Y. Abu-Kwaik. 2003. Comparative assessment of virulence traits in Legionella spp. Microbiology 149, 631-641   DOI   ScienceOn
17 Cianciotto, N.P., B.I. Eisenstein, C.H. Mody, and N.C. Engleberg. 1990. A mutation in the mip gene results in an attenuation of Legionella pneumophila virulence. J. Infect Dis. 162, 121-126   DOI   PUBMED   ScienceOn
18 Brand, B.C., A.B. Sadosky, and H.A. Shuman. 1994. The Legionella pneumophila icm locus: a set of genes required for intracellular mutiplication in human macrophages. Mol. Microbiol. 14, 797-808   DOI   ScienceOn
19 Vogel, J.P. and R.R. Isberg. 1999. Cell biology of Legionella pneumophila. Curr. Opin. Microbiol. 2, 30-34   DOI   ScienceOn
20 Cianciotto, N.P. 2001. Pathogenicity of Legionella pneumophila. Int. J. Med. Microbiol. 291, 331-343   DOI   ScienceOn