Browse > Article

Characterization and Composition of Ammonia-Oxidizing Bacterial Community in Full- Scale Wastewater Treatment Bioreactors  

Park, Hee-Deung (School of Civil, Environmental and Architectural Engineering, Korea University)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 112-118 More about this Journal
Abstract
Ammonia-oxidizing bacteria (AOB) are chemolithoautotrophs that play a key role in nitrogen removal from advanced wastewater treatment processes. Various AOB species inhabit and their community compositions vary over time in the wastewater treatment bioreactors. In this study, a hypothesis that operational and environmental conditions affect both the community compositions and the diversity of AOB in the bioreactors was proposed. To verify the hypothesis, the clone libraries based on ammonia monooxygenase subunit A were constructed using activated sludge samples from aerobic bioreactors at the Pohang, the Palo Alto, the Nine Springs, and the Marshall wastewater treatment plants (WWTPs). In those bioreactors, AOB within the Nitrosomonas europaea, N. oligotropha, N.-like, and Nitrosospira lineages were commonly found, while AOB within the N. communis, N. marina, and N. cryotolerans lineages were rarely detected in the samples. The AOB community structures were different in the bioreactors: AOB within the N. oligotropha lineage were the major microorganisms in the Pohang, the Palo Alto, and the Marshall WWTPs, while AOB within the N. europaea lineage were dominant in the Nine Springs WWTP. The correlations between the AOB community compositions of the wastewater treatment bioreactors and their operational (HRT, SRT, and MLSS) and environmental conditions (temperature, pH, COD, $NH_3$, and $NO_3{^-}$) were evaluated using a multivariate statistical analysis called the Redundancy Analysis (RDA). As a result, COD and $NO_3{^-}$ concentrations in the bioreactors were the statistically significant variables influencing the AOB community structures in the wastewater treatment bioreactors.
Keywords
ammonia-oxidizing bacteria; ammonia monooxygenase subunit A (amoA); nitrification; redundancy analysis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Park, H.D., L.M. Whang, S.R. Reusser, and D.R. Noguera. 2006. Taking advantage of aerated-anoxic operation in a full-scale University of Cape Town (UCT) process. Wat. Environ. Res. 78, 637-642   DOI   ScienceOn
2 Wagner, M., D.R. Noguera, S. Juretschko, G. Rath, H.P. Koops, and K.H. Schleifer. 1998. Combining fluorescent in situ hybridization (FISH) with cultivation and mathematical modeling to study population structure and function of ammonia-oxidizing bacteria in activated sludge. Wat. Sci. Technol. 37, 441-449   DOI   ScienceOn
3 Koops, H.P., B. Botcher, U.C. Moller, A. Pommerening-Roser, and G. Stehr. 1991. Classification of eight new species of ammoniaoxidizing bacteria: Nitrosomonas communis sp. nov., Nitrosomonas urea sp. nov., Nitrosomonas aestuarii sp. nov., Nitrosomonas marina sp. nov., Nitrosomonas nitrosa sp. nov., Nitrosomonas eutropha sp. nov., Nitrosomonas oligotropha sp. nov. and Nitrosomonas halophila sp. nov. J. Gen. Microbiol. 137, 1689-1699   DOI
4 Koops, H.P. and A. Pommerening-Roser. 2001. Distribution and ecophysiology of the nitrifying bacteria emphasizing cultured species. FEMS Microbiol. Ecol. 37, 1-9   DOI   ScienceOn
5 McCune, B. and J.B. Grace. 2002. Analysis of ecological communities. Gleneden Beach, OR, MJM Software Design
6 Park, H.D., S.Y. Lee, and S. Hwang. 2008. Redundancy analysis demonstrated the relevance of temperature to ammonia-oxidizing bacterial community compositions in a full-scale nitrifying bioreactor treating saline wastewater J. Microbiol. Biotechnol. in print   DOI   ScienceOn
7 이정수. 2005. 하.폐수처리: 최신 이론 및 응용. 서울, 도서출판 동화기술
8 Purkhold, U., A. Pommerening-Roser, S. Juretschko, M.C. Schmid, H.-P. Koops, and M. Wagner. 2000. Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys. Appl. Environ. Microbiol. 66, 5368-5382   DOI   ScienceOn
9 APHA, AWWA, and WPCF. 1989. Standard methods for the examination of water and wastewater. 17th ed. APHA, AWWA, WPCF. Washington, D.C., USA.
10 Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-4882   DOI   ScienceOn
11 Park, H.-D., J.M. Regan, and D.R. Noguera. 2002. Molecular analysis of ammonia-oxidizing bacterial populations in aerated-anoxic Orbal processes. Wat. Sci. Technol. 46, 273-280
12 Limpiyakorn, T., F. Kurisu, and O. Yagi. 2006. Quantification of ammonia-oxidizing bacteria populations in full-scale sewage activated sludge systems and assessment of system variables affecting their performance. Wat. Sci. Technol. 54, 91-99   DOI   ScienceOn
13 Limpiyakorn, T., Y. Shinohara, F. Kurisu, and O. Yagi. 2005. Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo. FEMS Microbiol. Ecol. 54, 205-217   DOI   ScienceOn
14 ter Braak, C.J.F. 1986. Canonical correspondence analysis: a new eigenvector technique for multivariate direct gradient analysis. Ecology 67, 1167-1179   DOI   ScienceOn
15 Dionisi, H.M., A.C. Layton, G. Harms, I.R. Gregory, K.G. Robinson, and G.S. Sayler. 2002. Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR. Appl. Environ. Microbiol. 68, 245-253   DOI   ScienceOn
16 Metcalf and Eddy. 2003. Wastewater engineering: treatment and reuse. 4th ed. New York, NY, McGraw-Hill
17 Kowalchuk, G.A. and J.R. Stephen. 2001. Ammonia-oxidizing bacteria: A model for molecular microbial ecology. Ann. Rev. Microbiol. 55, 485-529   DOI   ScienceOn
18 Saitou, N. and M. Nei. 1987. The neighbor-joining method - a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425   PUBMED   ScienceOn
19 Hiorns, W.D., R.C. Hastings, I.M. Head, A.J. McCarthy, J.R. Saunders, R.W. Pickup, and G.H. Hall. 1995. Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment. Microbiology 141, 2793-2800   DOI   ScienceOn
20 US-EPA. 1993. Manual: Nitrogen control. Cincinnati, OH, US-EPA.
21 Rotthauwe, J.H., K.P. Witzel, and W. Liesack. 1997. The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl. Environ. Microbiol. 63, 4704-4712   PUBMED   ScienceOn
22 Watson, S.W., E. Bock, H. Harms, H.-P. Koops, and A.B. Hooper. 1989. Nitrifying bacteria, pp. 1808-1834. In J.T. Staley, M.P. Bryant, N. Pfenning, and J.G. Holts (ed.), Bergey's Manual of Systematic Bacteriology, Williams & Wilkins, Baltimore, MD, USA.