Browse > Article

Methane Production and T-RFLP Patterns of Methanogenic Bacteria Dependent on Agricultural Methods  

Kim, Hun-Soo (Research Center, Humas Co., Ltd.)
Cho, Ju-Sik (Department of Biological Environment, Sunchon National University)
Park, Kyeong-Ryang (Department of Biotechnology, Hannam University)
Publication Information
Korean Journal of Microbiology / v.45, no.1, 2009 , pp. 17-25 More about this Journal
Abstract
We studied soil components, methane production, the number of methanogens, and T-RFLP patterns dependent on agricultural methods with the change of seasons. There is no regular increase or decrease tendency of the most soil components followed by sampling period. And the water content in soil was higher in October than May. Also a lot of methanogens existed in soil, and acetotrophs were relatively of smaller number than hydogenotrophs and formate utilizing methanogens using MPN (most probable number) enumeration. In the experiment using the formate, it was used from the first week, and only a minute amount was detecte after four weeks. However in the acetate, it was increased until the third week, and after that was consumed. And there was higher methane production for all soil samples which administered with the hydrogen spike. The activity of methanogens was higher in the organic and low-agrichemical agricultural method samples, and the organic agricultural method had high methanogen activity among the other samples. A result of T-RFLP pattern of mcrA gene digested with Sau96I, methanogen community have a little relation with agricultural methods and seasons. This results also agreed to no critical difference the soil components dependent on agricultural methods, but some analytical data have a positive relationship with a agricultural methods. Therefor we could concluded that the comparison study of community for soil bacteria sufficiently could be useful for the microbiological indicator.
Keywords
agricultural methods; methane production; methanogens; MPN; soil components; T-RFLP;
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Chin, K.J., T. Lueders, M.W. Friedrich, M. Klose, and R. Conrad. 2004. Archaeal community structure and pathway of methane formation on rice roots. Microb. Ecol. 47, 59-67   DOI   ScienceOn
2 Li, J., M. Wang, H. Yao, and Y. Wang. 2002. New estimates of methane emissions from Chinese rice paddies. Nutr. Cycl. Agroecosyst. 64, 33-42   DOI   ScienceOn
3 Liesack, W., S. Schnell, and N.P. Revsbech. 2000. Microbiology of flooded rice paddies. FEMS Microbiol. Rev. 24, 625-645   DOI   ScienceOn
4 Lueders, T. and M.W. Friedrich. 2002. Effects of amendment with ferrihydrite and gypsum on the structure and activity of methanogenic populations in rice field soil. Appl. Environ. Microbiol. 68, 2484-2494   DOI   ScienceOn
5 Mitra, A.P., P.K. Gupta, and C. Sharma. 2002. Refinement in methodologies for Methane budget estimation from Rice paddies. Nutrient Cycling Agroecosystems 64, 147-155   DOI   ScienceOn
6 Mphande, A.C., R.K. Malik, and P. Tauro. 1995. Methane emission and methanogen status of Indian rice soil. Biores. Technol. 55, 155-158   DOI   ScienceOn
7 Nakagawa, F., N. Yoshida, A. Sugimoto, E. Wada, T. Yoshioka, S. Ueda, and P. Vijarnsorn. 2002. Stable isotope and radiocarbon compositions of methane emitted from tropical rice paddies and swamps in Southern Thailand. Biogeochemistry 61, 1-19   DOI   ScienceOn
8 Touzel, J.P. and G. Albagnac. 1983. Isolation and characterization of Methanococcus mazei strani MC$_3$. FEMS Microbiol. Lett. 16, 241-245   DOI   ScienceOn
9 Wang, Z.Y., Y.C. Xu, Z. Li, Y.X. Guo, R. Wassmann, H.U. Neue, R.S. Lantin, L.V. Buendia, Y.P. Ding, and Z.Z. Wang. 2000. A four-year record of methane emissions from irrigated rice fields in the Beijing region of China. Nutr. Cycl. Agroecosyst. 58, 55-63   DOI   ScienceOn
10 Ward, D.M. and M.R. Winfrey. 1985. Interactions between methanogenic and sulfate reducing bacteria in sediments, pp. 141-179. In H.W. Jannasch and P.J. Williams (eds.), Advances in Aquatic Microbiology. Academic Press, London, UK
11 Min, H., Y.H. Zhao, M.C. Chen, and Y. Zhao. 1997. Methanogens in paddy rice soil. Nutr. Cycl. Agroecosyst. 49, 163-169   DOI   ScienceOn
12 Wassmann, R. and M.S. Aulakh. 2000. The role of rice plants in regulating mechanisms of methane missions. Biol. Fertil. Soils 31, 20-29   DOI   ScienceOn
13 Wind, T. and R. Conrad. 1997. Localization of sulfate reduction in planted and unplanted rice field soil. Biogeochemistry 37, 253-278   DOI   ScienceOn
14 Yao, H., K. Yagi, and I. Nouchi. 2000. Importance of physical plant properties on methane transport through several rice cultivars. Plant Soil 222, 83-93   DOI
15 Castro, H.F. 2003. Microbial ecology of anaerobic terminal carbon mineralization in Everglades soils, with emphasis on sulfate reducing prokaryotic assemblages. Ph. D. thesis. University of Florida, Gainesville, Florida, USA
16 Lee, K.B. 1997. Influence of different rice varieties on emission of methane in soil and exudation of carbohydrates in rhizosphere. Korean J. Soil Sci. Fert. 30, 257-264
17 Lu, Y., T. Lueders, M.W. Friedrich, and R. Conrad. 2005. Detecting active methanogenic populations on rice roots using stable isotope probing. Environ. Microbiol. 7, 326-336   DOI   ScienceOn
18 Luton, P.E., J.M. Wayne, R.J. Sharp, and P.W. Riley. 2002. The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill. Microbiology 148, 3521-3530   DOI   PUBMED   ScienceOn
19 Chauhan, A., A. Ogram, and K.R. Reddy. 2004. Syntrophic-methanogenic associations along a nutrient gradient in the Florida Everglades. Appl. Environ. Microbiol. 70, 3475-3484   DOI   ScienceOn
20 Satoh, A., M. Watanabe, A. Ueki, and K. Ueki. 2002. Physiological properties and phylogenetic affiliations of anaerobic bacteria isolated from roots of rice plants cultivated on a paddy field. Anaerobe. 8, 233-246   DOI   ScienceOn
21 Lu, W.F., W. Chen, B.W. Duan, W.M. Guo, R.S. Lantin, R. Wassmann, and H.U. Neue. 2000. Methane emissions and mitigation options in irrigated rice fields in southeast China. Nutr. Cycl. Agroecosyst. 58, 65-73   DOI   ScienceOn
22 Weber, S., T. Lueders, M.W. Friedrich, and R. Conrad. 2001. Methanogenic populations involved in the degradation of rice straw in anoxic paddy soil. FEMS Microbiol. Microbiol. Ecol. 38, 11-20   DOI   ScienceOn
23 Harada, N., M. Nishiyama, and S. Matsumoto. 2001. Inhibition of methanogens increases photo-dependent nitrogenase activities in anoxic paddy soil amended with rice straw. FEMS Microbiol. Ecol. 35, 231-238   DOI   ScienceOn
24 Lee, K.B. 1999. Methane emission among rice ecotypes in Korean paddy soil. Korean J. Environ. Agricul. 18, 1-5   과학기술학회마을   ScienceOn
25 Aulakh, M.S., J. Bodenbender, R. Wassmann, and H. Rennenberg. 2000. Methane transport capacity of rice plants. II. Variations among different rice cultivars and relationship with morphological characteristics. Nutr. Cycl. Agroecosyst. 58, 367-375   DOI   ScienceOn
26 Escoffier, S., B. Ollivier, J. LeMer, J. Garcin, and P. Roger. 1998. Evidence and quantification of thiosulfate reducers unable to reduce sulfate in rice field soils. Eur. J. Soil Biol. 34, 69-74   DOI   ScienceOn