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Isolation and Characterization of a Paenibacillus incheonensis YK5 with Antimicrobial Activity aginst MRSA  

Yoon, Young-Jun (Incheon Natural Environment Institute)
Kim, Hye-Yoong (Department of Biology, University of Incheon)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Kim, Jung-Wan (Department of Biology, University of Incheon)
Publication Information
Korean Journal of Microbiology / v.44, no.4, 2008 , pp. 326-332 More about this Journal
Abstract
Various bacteria were isolated from Korean soil samples based on their capability inhibiting the growth of MRSA strains. Among them, strain YK5 with the highest activity was a Gram positive sporulative bacillus with motility. It did not produce indole and no acid was formed from mannitol by the bacterium. The 16S rRNA sequence of the strain showed $95{\sim}98%$ homology with those of Paenibacillus spp.. The bacterial isolate shared the highest homology with that of P. elgii (98%), but was named as Paenibacillus incheonensis YK5 due to differences in physiological properties. Butanol extract of the P. incheonensis YK5 culture grown in SST medium at $37^{\circ}C$ for 96 hr showed a broad antimicrobial activity against Gram-positive (MRSA and Streptococcus pneumoniae) and negative (Pseudomonas aeruginosa, Salmonella spp., Shigella spp., Escherichia coli, Klebsiella pneumoniae) pathogenic bacteria and fungi (Cryptococcus neoformans and Trichophyton). The antimicrobial activity in the crude extract was stable in a broad range of temperature and pH, $20{\sim}100^{\circ}C$ and $3.0{\sim}6.0$, respectively. Therefore, the antimicrobial activity of P. incheonesis YK5 had potential as a novel antibiotics for pathogens including MRSA.
Keywords
16S rRNA; broad antimicrobial activity; MRSA; Paenibacillus incheonensis YK5;
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1 Falagas, M.E. and S.K. Kasiakou. 2005. Colistin: the revival of polymyxins for the management of multidrug-resistant gram negative bacterial infections. Clin. Infect. Dis. 40, 1333-1341   DOI   ScienceOn
2 Holt, J.G., N.R. Krieg, P.H.A. Sneaht, J.T. Staley, and S.T. Williams. 1994. Bergey's Manual of Determinative Bacteriology, 9th, Williams Wilkins, USA
3 Kim, D.S., C.Y. Bae, J.J. Jeon, S.J. Chun, H.W. Oh, S.G. Hong, K.S. Baek, E.Y. Moon, and K.S. Bae. 2004. Paenibacillus elgii sp. nov., with broad antimicrobial activity. Int. J. Syst. Evol. Microbiol. 54, 2031-2035   DOI   ScienceOn
4 Kim, K.M., J.H. Yoo, J.H. Choi, E.S. Park, K.S. Kim, K.S. Kim, S.R. Kim, S.M. Kim, H.J. Kim, J.S. Jung, K.H. Yoo, H.S. Oh, S.W. Yoon, M.R. Suh, Y.K. Yoon, J.Y. Lee, Y.K. Jang, H.Y. Jin, S.W. Kim, Y.R. Kim, Y.S. Kim, Y.S. Kim, J.U. Kim, J.M. Kim, K.R. Peck, H. Lee, M.D. Oh, S.H. Lee, W.K. Lee, S.H. Lee, M.H. Chung, S.I. Jung, H.J. Cheong, and W.S. Shin. 2006. Nationwide surveillance results of nosocomial infections along with antimicrobial resistance in intensive care units of sixteen university hospitals in Korea, 2004. Kor. J. Nosocomial Infect. Control. 11, 79-86
5 Nagai, K., K. Kanigiri, N. Arao, K. Suzumura, Y. Kawano, M. Yamaoka, H. Zhang, H. Zhang, M. Watanabe, and K. Suzuki. 2003. Novel thiopeptide antibiotics produced by Bacillus cereus isolated from a marine sponge. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological properties. J. Antibiot (Tokyo). 56, 123-128   DOI   PUBMED   ScienceOn
6 Nakamura, L.K. 1984. Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., nom. rev., Bacillus pabuli sp. nov., nom. rev., and Bacillus validus sp. nov., nom. rev. Int. J. Syst. Bacteriol. 34, 224-226   DOI
7 Nakamura, L.K. 1987. Bacillus alginolyticus sp. nov. and Bacillus chondroiinus sp. nov., two alginate-degrading species. Int. J. Syst. Bacteriol. 37, 284-286   DOI
8 Park, Y.J., J.S. Jeong, E.S. Park, E.S. Shin, S.H. Kim, and Y.S. Lee. 2007. Survey on the infection control of multidrug-resistant microorganisms in general hospitals in Korea. Kor. J. Nosocomial Infect Control. 12, 112-121
9 Slepecky, R.A. and H.E. Hemphill. 1991. The genus Bacillus-nonmedical. In The Prokaryotes, p. 1663-1696. In A. Balows, H.G. Truper, M. Dworkin, W. Harder, and K.H. Schleifer. Springer, New York, USA
10 Thomson, J.D., T.J. Gibso, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-4882   DOI
11 Centers for Disease Control and Prevention. 2002. Staphylococcus aureus resistant to vancomycin-United States, 2002 MMWR 51, 565-567
12 Rollema, H.S., O.P. Kuipers, P. Both, W.M. De Vos, and R.J. Siezen. 1995. Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering. Appl. Environ. Microbiol. 61, 2873-2878   PUBMED
13 Gedney, J. and R.W. Lacey. 1982. Properties of methicillin-resistant staphylococci now endemic in Australia. Med. J. Aust. 1, 448-450   PUBMED
14 Martin, N.I., H. Hu, M.M. Moake, J.J. Churey, R. Whittal, R.W. Worobo, and J.C. Vederas. 2003. Isolation, structural characterization, and properties of mattacin (polymyxin M), a cyclyc peptide antibiotic produced by Paenibacillus kobensis M. J. Biol. Chem. 278, 13124-13132   DOI   ScienceOn
15 Saravolatz, L.D., N. Markowitz, L. Arking, D. Pohlod, and E. Fisher. 1982 Methicillin-resistant Staphylococcus aureus Epidemicologic observations during a community-acquired outbreak. Ann. Intern. Med. 96, 11-16   DOI   PUBMED   ScienceOn
16 Song, J.H., J.W. Yang, J.H. Joung, S.J. Kang, and N.Y. Lee. 2000. Unique alterations in Penicillin-binding protein 2B of multidrugresistant Streptococcus pneumoniae from Korea. Kor. J. Infect. Dis. 32, 108-114
17 Wenzel, R.P. 1982. The emergence of methicillin-resistant Staphylococcus aureus. Ann. Intern. Med. 97, 440-442   DOI   PUBMED   ScienceOn
18 Chung, Y.R., C.H. Kim, I. Hwang, and J. Chun. 2000. Paenibacillus koreensis sp. nov., a new species that produces an iturin-like antifungal compound. Int. J. Syst. Evol. Microbiol. 50, 1495-1500   DOI   PUBMED   ScienceOn
19 Vijayakumar, E.K., J. Kenia, T. Mukhopadhyay, and S.R. Nadkarni. 1999. Methylsulfomycin I, a new cyclic peptide antibiotic from a Streptomyces sp. HIL Y-9420704. J. Nat. Prod. 62, 1562- 1564   DOI   ScienceOn
20 Michael, A.G. 2000. Infection prevention and control unit and the division of infectious disease, department of medicine, the University Health Networt, University of Toronto, Ontario. Is methicillinresistant Staphylococcus aureus an emerging community pathogen? A review of the literature. Can. J. Infect. Dis. 11, 202-211   DOI
21 Yoon, Y.J., K.H. Im, Y.H. Koh, S.K. Kim, and J.W. Kim. 2003. Genotyping of six pathogenic Vibrio species based on RFLP of 16S rDNAs for rapid identification. J. Microbiol. 41, 312-319
22 Hiramatsu, K., N. Aritaka, H. Hanaki, S. Kawasaki, Y. Hosoda, S. Hori, Y. Fukuchi, and I. Kobayashi. 1997. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet 350, 1670-1673   DOI   ScienceOn
23 Selim, S., J. Negrel, C. Govaerts, S. Gianinazzi, and D. Van Tuinen. 2005. Isolation and partial characterization of antagonistic peptides produced by Paenibacillus sp. strain B2 isolated from the sorghum mycorrhizosphere. Appl. Environ. Microbiol. 71, 6501-6507   DOI   ScienceOn
24 Govaerts, C., J. Orwa, A. Van Schepdael, E. Roets, and J. Hoogmartens. 2002. Characterization of polypeptide antibiotics of the polymyxin series by liquid chromatography electrospray ionization ion trap tandem mass spectrometry. J. Pept. Sci. 8, 45-55   DOI   ScienceOn
25 Aram, J. and A.E. Glatt. 1998. True community-acquired methicillin- resistant Staphylococcus aureus bacteremia. Infect. Control. Hosp. Epidemiol. 19, 106-107   DOI   ScienceOn
26 He, Z., D. Kisla, L. Zhang, C. Yuan, K.B. Green-Church, and A.E. Yousef. 2007. Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin. Appl. Environ. Microbiol. 73, 168-178
27 Heyndrickx, M., K. Vandemeulebroecke, B. Hoste, P. Janssen, K. Kersters, P. De Vos, N.A. Logan, N. Ali, and R.C.W. Berkeley. 1996. Reclassification of Paenibacillus (formerly Bacillus) pulvifaciens (Nakamura 1966) Ash et al. 1994, a later subjective synonym of Paenibacillus (formerly Bacillus) larvae (White 1984) Ash et al. 1994, as a subspecies of P. larvae, with emended description of P. larvae as P. larvae subsp. larvae and P. larvae subsp. pulvifaciens. Int. J. Syst. Bacteriol. 46, 270-279
28 Waksman, S.A. and A.T. Heinrich. 1943. The nomenclature and classification of the Actinomycetes. J. Bacteriol. 46, 337-341   PUBMED
29 Kim, M.N., C.H. Pai, J.H. Woo, J.S. Ryu, and K. Hiramatsu. 2000. Vancomycin resistant Staphylococcus aureus in Korea. J. Clin. Microbiol. 38, 3879-3881   PUBMED
30 Noble, W.C., Z. Virani, and R.G.A Cree. 1992. Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiol. Lett. 72, 195-198   PUBMED
31 Nielson, P. and J. Sorensen. 1997. Multi-target and mediumindependent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere. FEMS Microbiol. Ecol. 22, 183-192   DOI
32 Berdy, J. 1989. The discovery of new bioactive microbial metabolites : Screening and identification. In M.E. Bushell and U. Grafe (eds.), Bioactive metabolites from microorganisms, Elsevier, Amsterdam. 3, 25
33 Falagas, M.E. and S.K. Kasiakou. 2006. Toxicity of polymyxins: a systematic review of the evidence from old and recent studies. Crit. Care. 10, R27
34 Hiramatsu, K. 2001. Vancomycin resistant Staphylococcus aureus : a new model of antibiotic resistance. J. Infect. Dis. 1, 1-16
35 Jeffrey, W.W., S. Tallapragada, P. Farrel, and L.M. Dembry. 1996. Comparison of rectal and perirectal swabs for detection of colonization with vancomycin-resistant Enterococci. J. Clin. Microbiol. 34, 210-212   PUBMED
36 National Nosocomial Infections Surveillance System. 2004. National Nosocomial Infection Surveillance (NNIS) system report, data summary from January 1992 through June 2004, issued October 2004. Am. J. Infect. Control. 32, 470-485   DOI   ScienceOn
37 Sambrook, J., E.F. Fritsch, and T. Mamiatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., USA
38 Davis, S.L., M.B. Perri, S.M. Donabedian, C. Manierski, A. Singh, D. Vager, N.Z. Haque, K. Speirs, R.R. Muder, B. Robinson-Dunn, M.K. Hayden, and M.J. Zervos. 2007. Epidemiology and outcomes of community-associated methicillin-resistant Staphylococcus aureus. Infection. J. Clin. Microbiol. 45, 1705-1711
39 Ash, C., F.G. Priest, and M.D. Collins. 1993. Molecular identifica-tion of rRNA group 3 bacilli (Ash, Farrow, Wallbanks, and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 64, 253-260   DOI