Browse > Article

Angiogenic Effect of Cardiac Ankyrin Repeat Protein Overexpression in Vascular Endo-thelial Cell  

Kong, Hoon-Young (Department of Molecular Biology, BK21 Graduate Program for RNA Biology, Institute of Nanosensor and Biotechnology, Dankook University)
Byun, Jong-Hoe (Department of Molecular Biology, BK21 Graduate Program for RNA Biology, Institute of Nanosensor and Biotechnology, Dankook University)
Publication Information
Korean Journal of Microbiology / v.44, no.4, 2008 , pp. 282-288 More about this Journal
Abstract
Tissue ischemia resulting from the constriction or obstruction of blood vessels leads to an illness that may affect many organs including the heart, brain, and legs. In recent years, considerable progress has been made in the field of therapeutic angiogenesis and the new approaches are expected to cure those "no-option patients" who are unsuited to conventional therapies. Although single angiogenic growth factor may be successful in inducing angiogenesis, combination of multiple growth factors is increasingly sought these days to augment the therapeutic responses. This trend is proper in light of the fact that blood vessel formation is a complex and multi-step process that requires the actions of many different factors. To meet the growing need for functionally significant blood flow recovery in the ischemic tissues, a novel strategy that can provide concerted actions of multiple factors is required. One way to achieve such a goal is to use a transcription factor that can orchestrate the expression of multiple target genes in the ischemic region and thus induce significant level of angiogenesis. Here, a putative transcription factor, cardiac ankyrin repeat protein (CARP), was evaluated in adenoviral vector context for angiogenic activity in human umbilical vein endothelial cells. The results indicated significant increase in proliferation, capillary-like structure formation, and induction of vascular endothelial growth factor, a typical angiogenic gene. Taken together, these results suggest that CARP represents itself as a novel target for therapeutic angiogenesis and warrants further investigation.
Keywords
angiogenesis; blood vessel; cardiac ankyrin repeat protein; endothelial cell; gene therapy;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Banai, S., M.T. Jaklitsch, M. Shou, D.F. Lazarous, M. Scheinowitz, S. Biro, S.E. Epstein, and E.F. Unger. 1994. Angiogenic-induced enhancement of collateral blood flow to ischemic myocardium by vascular endothelial growth factor in dogs. Circulation 89, 2183-2189   DOI   PUBMED   ScienceOn
2 Chu, W., D.K. Burns, R.A. Swerlick, and D.H. Presky. 1995. Identification and characterization of a novel cytokine-inducible nuclear protein from human endothelial cells. J. Biol. Chem. 270, 10236-10245   DOI
3 Heilmann, C.A., T. Attmann, A. Thiem, E. Haffner, F. Beyersdorf, and G. Lutter. 2003. Gene therapy in cardiac surgery: intramyocardial injection of naked plasmid DNA for chronic myocardial ischemia. Eur. J. Cardiothorac. Surg. 24, 785-793   DOI   ScienceOn
4 Libby, P. 2003. Vascular biology of atherosclerosis: overview and state of the art. Am. J. Cardiol. 91, 3A-6A
5 Shi, Y., B. Reitmaier, J. Regenbogen, R.M. Slowey, S.R. Opalenik, E. Wolf, A. Goppelt, and J.M. Davidson. 2005. CARP, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue. Am. J. Pathol. 166, 303-312   DOI   PUBMED   ScienceOn
6 Bang, M.L., R.E. Mudry, A.S. McElhinny, K. Trombitas, A.J. Geach, R. Yamasaki, H. Sorimachi, H. Granzier, C.C. Grogorio, and S. Labeit. 2001. Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies. J. Cell Biol. 153, 413-427   DOI
7 Corpet, F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16, 10881-10890   DOI
8 Lee, Y.S., H.S. Jang, J.M. Kim, J.S. Lee, J.Y. Lee, K.L. Kim, I.S. Shin, W. Suh, J.H. Choi, E.S. Jeon, J. Byun, and D.K. Kim. 2005. Adenoviral-mediated delivery of early growth response factor-1 gene increases tissue perfusion in a murine model of hindlimb ischemia. Mol. Ther. 12, 328-336   DOI   ScienceOn
9 Ruel, M. and F.W. Sellke. 2003. Angiogenic protein therapy. Semin. Thorac. Cardiovasc. Surg. 15, 222-235   DOI   ScienceOn
10 De Waard, V., T.A. Van Achterberg, N.J. Beauchamp, H. Pannekoek, and C.J. De Vries. 2003. Cardiac Ankyrin Repeat Protein (CARP) expression in human and murine atherosclerotic leison: activin induces CARP in smooth muscle cells. Arterioscler Tromb. Vasc. Biol. 23, 64-68   DOI   ScienceOn
11 Zou, Y., S. Evans, J. Chen, H.C. Kuo, R.P. Harvey, and K.R. Chien. 1997. CARP, a cardiac ankyrin repeat protein, is downstream in the Nkx2-5 homeobox gene pathway. Development. 124, 793-804
12 Lazarous, D.F., M. Shou, M. Scheinowitz, E. Hodge, V. Thirumurti, A.N. Kitsiou, J.A. Stiber, A.D. Lobo, S. Hunsberger, E. Guetta, S.E. Epstein, and E.F. Unger. 1996. Comparative effects of basic fibroblast growth factor and vascular endothelial growth factor on coronary collateral development and the arterial response to injury. Circulation 94, 1074-1082   DOI   PUBMED   ScienceOn
13 Simeonidis, S., D. Stauber, G. Chen, W.A. Hendrickson, and D. Thanos. 1999. Mechanisms by which IkappaB proteins control NF-kappaB activity. Proc. Natl. Acad. Sci. USA 96, 49-54
14 Baumeister, A., S. Arber, and P. Caroni. 1997. Accumulation of muscle ankyrin repeat protein transcript reveals local activation of primary myotube endcompartments during muscle morphogenesis. J. Cell Biol. 139, 1231-1242   DOI
15 Engelse, M.A., J.H. Lardenoye, J.M. Neele, J.M. Grimbergen, M.R. De Vries, M.L. Lamfers, H. Pannekoek, P.H. Quax, and C.J. De Vries. 2002. Adenoviral activin A expression prevents intimal hyperplasia in human and murine blood vessels by maintaining the contractile smooth muscle cell phenotype. Circ. Res. 90, 1128-1134   DOI   ScienceOn
16 박정의. 2006. 죽상경화증의 병태생리. HANYANG MEDICAL REVIEWS 26, 4-10
17 Kanai, H., T. Tanaka, Y. Ailara, S. Takeda, M. Miyazono, R. Nagai, and M. Kurabayashi. 2001. Transforming growth factorbeta/ Smads signaling induces transcription of the cell type restricted ankyrin repeat protein CARP gene through CAGA motif in vascular smooth muscle cells. Circ. Res. 88, 30-36   DOI   PUBMED   ScienceOn
18 Kim, H.J., S.Y. Jang, J.I. Park, J. Byun, D.I. Kim, Y.S. Do, J.M. Kim, S. Kim, B.M. Kim, W.B. Kim, and D.K. Kim. 2004. Vascular endothelial growth factor-induced angiogenic gene therapy in patients with peripheral artery disease. Exp. Mol. Med. 36, 336-344   DOI   PUBMED   ScienceOn
19 Aihara, Y., M. Kurabayashi, Y. Saito, Y. Ohyama, T. Tanaka, S. Takeda, K. Tomaru, K. Sekiguchi, M. Arai, T. Nakamura, and R. Nagai. 2000. Cardiac ankyrin repeat protein is a novel marker of cardiac hypertrophy: role of M-CAT element within the promoter. Hypertension 36, 48-53   DOI   ScienceOn
20 Miller, M.K., M.L. Bang, C.C. Witt, D. Labeit, C. Trombitas, K. Watanabe, H. Granzier, A.S. McElhinny, C.C. Gregorio, and S. Labeit. 2003. The muscle ankyrin repeat proteins: CARP, ankrd2/ Arpp and DARP as a family of titin filament-based stress response molecules. J. Mol. Biol. 333, 951-964   DOI   ScienceOn
21 Zolk, O., M. Marx, E. Jckel, A. El-Armouche, and T. Eschenhagen. 2003. Beta adrenergic stimulation induces cardiac ankyrin repeat protein expression: involvement of protein kinase A and calmodulin-dependent kinase. Cardiovasc. Res. 59, 563-572   DOI   ScienceOn
22 Isner, J.M., P.R. Vale, J.F. Symes, and D.W. Losordo. 2001. Assessment of risks associated with cardiovascular gene therapy in human subjects. Circ. Res. 89, 389-400   DOI